LOCUS       Exported     3328 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3328
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1366..1413
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3261..3277
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3278..3309
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1294
                     /note="The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis."
                     /translation="MNIINLINKKQRGKALNLAEINWFVNAVLNKTIADYQITAFLMAIWFKGMNPNELFLLTKAMVDTGEIIKFNHHGKISVDKHSTGGIGDKVSLALVPILTSLGFSVAKLSGRGLGYTGGTIDKLEAVGVKTELTDQQAQACLDKNDCFIIGQSKDIAPVDKVLYGLRDITGTVDSLPLIASSIMSKKLAVMNEYIFIDLKYGKGAFCKTKKIANELAKLMQSIAKSFKRKLSVKLSDMNQVLGKAVGNVIEVNEAVNFLKQDLDQVGQDFIDLMQTIVINILLETKQAKTKQKAIELYQDVLTSKKAWNRFLSFIESQGGNVELFTQKEGFFKPKYKASIKAEKSGILHFTDPIDLAKIGINLGAGRMKKTDQIDPMAGLFLMKKDNESVAVGDTVLNLYSSSPISNEYISAAQKTIIINK"
                     /label="MG_RS00260"
     primer_bind     complement(1418..1434)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1501..2089)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2280..3140)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3141..3245)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GAATATTATC AACCTGATTA ACAAGAAGCA
       61 ACGTGGCAAA GCACTGAACC TGGCAGAGAT CAACTGGTTT GTGAATGCGG TCCTGAACAA
      121 GACGATCGCT GACTACCAGA TTACCGCGTT TTTAATGGCA ATTTGGTTCA AGGGTATGAA
      181 TCCAAATGAA CTGTTTCTGC TGACGAAGGC AATGGTGGAT ACGGGCGAGA TTATTAAGTT
      241 TAACCACCAC GGTAAAATTT CTGTCGATAA GCACAGCACC GGTGGTATTG GTGACAAAGT
      301 CAGCCTGGCG CTGGTCCCGA TCTTGACCTC GCTGGGTTTC TCAGTCGCCA AGCTGAGCGG
      361 TCGTGGCCTG GGCTACACGG GTGGTACGAT CGACAAGTTG GAAGCTGTTG GTGTGAAAAC
      421 CGAGCTGACG GACCAGCAAG CACAAGCTTG CCTGGATAAG AATGATTGCT TTATCATTGG
      481 TCAAAGCAAA GATATCGCCC CGGTCGATAA GGTTCTGTAC GGTTTGCGCG ACATCACCGG
      541 TACGGTGGAT AGCCTGCCGC TGATCGCGAG CTCCATTATG AGCAAAAAAC TGGCGGTTAT
      601 GAATGAGTAC ATCTTCATCG ATTTGAAATA CGGCAAAGGT GCATTTTGCA AGACGAAGAA
      661 AATCGCCAAT GAGCTGGCTA AACTGATGCA ATCTATCGCC AAGAGCTTTA AACGTAAATT
      721 GTCAGTCAAA CTGAGCGATA TGAACCAAGT GCTGGGCAAG GCGGTTGGCA ATGTTATCGA
      781 AGTGAATGAA GCAGTCAATT TCCTGAAGCA GGACCTGGAC CAAGTCGGTC AGGATTTCAT
      841 TGATTTGATG CAGACCATTG TCATTAACAT TCTGCTGGAA ACCAAGCAAG CAAAGACGAA
      901 ACAGAAGGCC ATCGAGCTGT ATCAAGATGT TCTGACCTCC AAGAAGGCAT GGAATCGCTT
      961 TCTGTCGTTC ATTGAGAGCC AAGGCGGCAA TGTTGAGTTG TTCACGCAGA AGGAAGGTTT
     1021 CTTTAAACCG AAATATAAAG CAAGCATTAA GGCAGAAAAG AGCGGCATCC TGCACTTCAC
     1081 CGACCCGATC GATCTGGCTA AAATCGGCAT CAATCTGGGC GCAGGCCGCA TGAAGAAAAC
     1141 CGATCAGATC GATCCGATGG CCGGTCTGTT CCTGATGAAG AAGGATAATG AGAGCGTCGC
     1201 GGTGGGCGAC ACGGTGTTGA ACCTGTACAG CAGCTCTCCG ATTAGCAACG AATACATTAG
     1261 CGCCGCGCAA AAGACGATCA TTATCAACAA GTGAAGAGCT TAGAGACCGT GTTCTATGTC
     1321 CCCATCGTCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1381 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1441 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     1501 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1561 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1621 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1681 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1741 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1801 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1861 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1921 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1981 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2041 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     2101 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2161 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     2221 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2281 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2341 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     2401 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     2461 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     2521 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     2581 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     2641 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     2701 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     2761 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2821 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2881 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     2941 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     3001 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3061 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3121 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3181 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3241 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3301 CTTTTGACTT GGTCAGGTGG AGTGGGAG
//