LOCUS       Exported     3469 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3469
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1507..1554
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3402..3418
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3419..3450
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1435
                     /label="MG_RS00520"
                     /note="Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity."
                     /translation="MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSGWKPGELIVIAARPGRGKTALLINFMASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS"
     primer_bind     complement(1559..1575)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1642..2230)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2421..3281)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3282..3386)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GGGTCAACAG ACGTCCTTTA AGTATGCGAA
       61 TGATAGCAAT ATCGAGAGAG CGGAACGCCG TCTGATGCAG GCGGTGGCGC AAAATTCAGA
      121 GGGTATCGAT CTGATCTTCA ATAAACTGGA ACCGATTGAC TTTTTTGCTA CCCCGTTCAA
      181 GCTGATCTTC CAAACCGCAA AGGAGAATTA CCAATTGAAC AATCCTATTA TTGGCTCTGG
      241 TTTGCTGGAG GCGGTGAAGT TCAAGTTGGA TGCCAATGAT CAAAGCACGA AAAGCGAGCT
      301 GGAAATTCTG TTTACCAAAA TCCTGCTGAT TCGTCTGCCG CCGAATCAAA CTGAAATCAA
      361 AACTCTGGTT GACGTCGTTA AAAAAGCAAG CATCTTTCGT CGTCTGCAAC AATTCGCTAA
      421 ACGTGTTTAC AACGAAGAGT TCAAGCTGAA GGAGGATCGT TTTGAAGGTT ACTTGCAAGC
      481 GATCCAAGAT GATTTTGTGA AAATCATTCA CTCTGCATTT AGCAACATTT TTGCCTTCAG
      541 CTACGATGAG ATTGCCAACC AAGAGGAAGC CCTGATCAAG AAAGTTCACC GTGGTGAACT
      601 GATTATCAGC GGTCTGAGCA GCGGCTTCTT AAAATTGGAC CAATTGACCT CGGGTTGGAA
      661 GCCGGGTGAG CTCATCGTTA TCGCGGCGCG CCCGGGCCGC GGTAAGACCG CGCTGCTGAT
      721 TAATTTTATG GCGAGCGCCG CGAAGCAAAT TGACCCAAAG ACCGATGTTG TGCTGTTTTT
      781 CAGCCTGGAG ATGCGTAACC GCGAGATCTA CCAACGTCAT CTCATGCATG AGTCCCAAAC
      841 CAGCTACACT CTGACGAATA GACAACGTAT CAACAATGTG TTCGAAGAAT TAATGGAGGC
      901 GAGCTCCCGT ATCAAGAATC TGCCGATCAA GCTGTTCGAC TATAGCAGCC TGACCTTGCA
      961 GGAGATCCGT AACCAAATCA CGGAAGTGTC CAAGACGTCC AACGTGCGTC TGGTCATCAT
     1021 CGATTATCTG CAACTGGTTA ATGCATTGAA AAACAACTAC GGTCTGACCC GTCAGCAGGA
     1081 AGTTACGATG ATCAGCCAGT CCCTGAAAGC GTTCGCGAAA GAATTTAACA CGCCGATCAT
     1141 TGCGGCCGCT CAGCTGAGCC GCCGCATCGA GGAGCGTAAG GATTCCCGTC CGATCCTGTC
     1201 CGATCTCCGT GAGTCGGGCA GCATCGAGCA GGACGCTGAT ATGGTTTTGT TTATCCACCG
     1261 TACCAATGAT GATAAGAAAG AGCAAGAAGA GGAGAATACC AATCTGTTCG AAGTGGAACT
     1321 GATTCTGGAG AAGAACCGCA ATGGCCCGAA CGGCAAGGTG AAACTCAATT TCCGTAGCGA
     1381 TACTAGCAGC TTTATTTCTC AGTACAGCCC GAGCTTTGAC GACCAGTATT CCTGAAGAGC
     1441 TTAGAGACCG ACCCGTCCTT TGTTGTGATC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1501 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1561 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1621 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1681 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1741 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1801 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1861 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1921 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1981 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     2041 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     2101 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     2161 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     2221 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     2281 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     2341 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     2401 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2461 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2521 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2581 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2641 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2701 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2761 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2821 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2881 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2941 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     3001 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     3061 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     3121 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     3181 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     3241 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     3301 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3361 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3421 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//