LOCUS       Exported     4240 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4240
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2278..2325
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     4173..4189
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4190..4221
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2206
                     /label="MG_RS00575"
                     /note="3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs."
                     /translation="MKVLTELQKQIFTIVKKENGKPIPPGIVVRMMENSPNFPGKHLIYRAIDDLLDWAILRKAGGVTNQLLVNYEPAEPLLDKKLQGILTLGNKNSGFIRSLDDDKTVYYVHYSNLTGALDGDLVEFCKLDKPQFGDKFDAAVITILKRARILYAGNFLVDQNEFALEYKIVADNPRFYLTMIVNPDSIPNNLASNTKIAFQIDEYDPDNNLCKVSVQQVLGNNDDPLINIKAIMLDNSIVFETNDVVEQHANKLSFDTEEQHKAYRQDLTDLAFVTVDPTTSKDLDDAIYVKTIPTGFVLYVAIADVAHYVNRNSEIDIEAKHKTSSIYLPGHYVVPMLPEQLSNQLCSLNPAQKRYVVVCEISFDNQGRIKTNKLYPATIISKNRFSYDQVNKWLNNKSELNCDETVINSLKAAFTLSDLIQAQRQKRGTIDLSHKETEIVVDEHYFPIKINFLVHDKAETMIENLMVVANETVAWVLTNNKIALPYRVHPRPSKKKLQSLIETVGELNITKPQFNLDTVTSSQIASWLNENKDNPSYEIFVILLLRTLGKAFYSVNPLMHFSIGSNHYTHFTSPIRRYIDLTIHRLLWMHLFTPDQFTDNERDQLKQELEKIADTVNDTEIKIINCERNANDYLTTLLLSKQIGKTFSGFISAITSFGIFMRMDENNFDGLIKITTIPDDFFIFEKEKMVLKGRKTNKVYKIGDRLEAKLSEIDFIQKRAILTLI"
     primer_bind     complement(2330..2346)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(2413..3001)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3192..4052)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4053..4157)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GAAAGTGCTG ACGGAACTGC AAAAGCAGAT
       61 TTTTACCATT GTTAAAAAAG AGAATGGTAA ACCGATTCCA CCGGGCATCG TCGTTCGTAT
      121 GATGGAGAAT TCCCCGAACT TTCCGGGCAA GCACCTGATT TATCGTGCCA TTGATGATCT
      181 GCTGGACTGG GCCATTCTGC GCAAAGCGGG TGGTGTTACC AATCAGCTGC TGGTGAACTA
      241 CGAACCGGCC GAGCCGCTGC TGGACAAGAA ATTGCAAGGT ATTCTGACGC TGGGCAATAA
      301 GAACTCTGGC TTCATCCGCA GCCTGGATGA CGATAAAACG GTCTACTATG TTCACTACAG
      361 CAACCTGACT GGTGCATTGG ACGGTGACCT CGTGGAGTTT TGCAAGCTGG ATAAGCCACA
      421 ATTCGGCGAT AAATTTGACG CAGCCGTCAT TACCATCCTG AAGCGTGCGC GCATCCTGTA
      481 CGCGGGCAAC TTTCTGGTAG ACCAAAACGA ATTCGCCCTG GAATACAAGA TTGTGGCAGA
      541 CAACCCGCGC TTTTACCTGA CCATGATCGT CAACCCTGAT AGCATTCCGA ACAATCTGGC
      601 CAGCAATACC AAAATTGCCT TCCAGATTGA CGAGTACGAC CCGGATAACA ATCTGTGTAA
      661 GGTGAGCGTT CAACAAGTTC TGGGTAATAA TGACGACCCG CTGATCAATA TTAAGGCTAT
      721 TATGCTGGAT AACAGCATCG TTTTCGAAAC CAACGATGTT GTGGAGCAAC ACGCGAACAA
      781 GCTGAGCTTC GATACGGAGG AGCAACACAA GGCGTACCGT CAGGACCTGA CCGACCTGGC
      841 GTTCGTCACC GTGGATCCGA CTACGAGCAA AGACCTGGAT GACGCGATTT ACGTCAAAAC
      901 CATCCCGACC GGTTTCGTTC TGTACGTTGC AATCGCGGAT GTGGCGCACT ACGTAAACCG
      961 TAACAGCGAG ATCGATATTG AAGCAAAACA CAAAACCTCC TCGATCTATC TGCCGGGCCA
     1021 CTACGTCGTG CCGATGCTGC CGGAGCAGCT GAGCAATCAG CTGTGTAGCC TGAACCCGGC
     1081 TCAGAAGCGC TACGTTGTTG TTTGTGAGAT CAGCTTTGAC AATCAGGGTC GTATTAAAAC
     1141 CAACAAACTG TACCCGGCTA CCATTATTAG CAAGAACCGT TTCAGCTACG ATCAAGTGAA
     1201 CAAATGGTTG AACAACAAGA GCGAACTGAA TTGTGACGAA ACGGTTATCA ACTCTCTCAA
     1261 AGCAGCCTTC ACGCTGAGCG ATCTGATCCA AGCTCAGCGT CAAAAACGTG GCACTATTGA
     1321 CCTGAGCCAC AAGGAAACCG AAATTGTCGT GGATGAGCAT TATTTCCCGA TTAAGATTAA
     1381 CTTCCTGGTT CACGATAAGG CGGAAACCAT GATCGAAAAT CTGATGGTCG TTGCGAATGA
     1441 AACGGTTGCG TGGGTTCTGA CCAATAATAA AATTGCATTA CCGTACCGTG TGCACCCACG
     1501 TCCGAGCAAA AAGAAGCTGC AGTCCCTGAT CGAAACGGTT GGCGAGCTGA ACATTACCAA
     1561 GCCGCAGTTC AACCTGGATA CTGTGACCAG CAGCCAGATC GCGTCCTGGC TGAATGAAAA
     1621 CAAGGATAAT CCGAGCTACG AGATTTTTGT TATTCTGCTG CTGCGTACCC TGGGCAAAGC
     1681 CTTCTACTCC GTGAACCCGC TGATGCACTT CAGCATTGGT TCCAATCATT ACACCCATTT
     1741 CACCAGCCCT ATCCGTCGTT ACATCGATCT GACTATTCAT CGTTTGCTGT GGATGCACCT
     1801 CTTTACCCCT GACCAGTTCA CCGATAACGA ACGCGACCAG TTGAAACAGG AACTGGAGAA
     1861 AATTGCAGAT ACGGTCAATG ACACGGAGAT TAAGATTATT AACTGTGAGC GTAACGCGAA
     1921 TGATTATCTG ACGACCCTGC TGCTCTCTAA GCAAATTGGT AAAACCTTCA GCGGTTTTAT
     1981 TTCGGCGATT ACCAGCTTTG GTATCTTCAT GCGCATGGAC GAGAATAACT TTGATGGTTT
     2041 GATTAAGATC ACTACCATCC CGGACGACTT TTTTATTTTT GAGAAGGAGA AGATGGTGCT
     2101 GAAGGGTCGC AAAACCAACA AAGTATACAA GATCGGTGAC CGCCTGGAAG CTAAGCTGAG
     2161 CGAAATTGAT TTTATCCAGA AGCGCGCTAT TCTGACCCTG ATTTGAAGAG CTTAGAGACC
     2221 CGCTGATGGT AATTAGCCCT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     2281 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     2341 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     2401 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     2461 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     2521 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     2581 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     2641 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     2701 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     2761 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     2821 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     2881 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2941 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     3001 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     3061 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     3121 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     3181 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     3241 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     3301 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     3361 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     3421 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     3481 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     3541 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     3601 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     3661 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     3721 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     3781 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     3841 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     3901 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     3961 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     4021 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     4081 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     4141 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     4201 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//