LOCUS       Exported     3358 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3358
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1396..1443
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3291..3307
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3308..3339
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1324
                     /label="MG_RS00610"
                     /note=""
                     /translation="MSDKLLTIDLSHVYGFDKEIIFKKYQKKVDQIHQDFLAHKLADGHMTGWYDQPDQNHQFLLKTINQIDKKFKSLKVTDIVYVGIGGSFTGIKTVLDFLKPKQRTGLKIHFVPDLSAFQAASVIKEIKNKSWALITTSKSGRTLEPALNFRIFRNLLNKRYGNKHYQRVVVITDEKKGLLTKMASNHGYQKLVIDSNIGGRFSTLSPAGLLLAKLFGHDPKAILKGTLQAKKDLQTTSLENNSAYLYAVVRHWLYTTKKFKIEVCIAYHSLYEYLLLQHRQLFGESEGKNDKSLFPTFSIFTVDLHSMGQLYQEGEKVFFETVIDVKNPLVNINLPPSDFDNDDELDFLLDKSLNEISDVAIDSVIKAHYQANVSIIKLTLKEQSAFMFGYFYFWLSVATVMSGSLLGHNVFNQPGVEVYKKLMFEKLRSGH"
     primer_bind     complement(1448..1464)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1531..2119)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2310..3170)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3171..3275)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GTCAGATAAA CTGCTGACCA TTGATCTGTC
       61 TCACGTATAC GGTTTCGACA AGGAAATTAT TTTCAAAAAG TACCAAAAGA AGGTTGATCA
      121 GATTCACCAA GACTTCCTGG CCCATAAACT GGCGGACGGT CATATGACCG GTTGGTACGA
      181 TCAACCTGAC CAAAATCACC AGTTTCTGTT GAAAACCATT AACCAAATTG ACAAGAAGTT
      241 CAAGAGCTTG AAGGTGACCG ACATTGTCTA CGTTGGTATT GGCGGCAGCT TCACGGGTAT
      301 TAAGACCGTT CTGGATTTTT TGAAACCGAA ACAGCGCACG GGCCTGAAGA TCCACTTTGT
      361 CCCGGACCTG AGCGCTTTTC AGGCTGCGTC TGTCATCAAA GAAATTAAGA ACAAATCGTG
      421 GGCGTTGATC ACCACCAGCA AGTCCGGTCG CACGCTGGAA CCGGCGCTGA ATTTTCGTAT
      481 TTTCCGTAAT CTGCTGAACA AGCGCTATGG CAACAAACAC TATCAGCGTG TTGTGGTCAT
      541 TACCGACGAG AAAAAGGGCC TGCTGACGAA AATGGCCAGC AACCATGGCT ACCAGAAACT
      601 GGTCATCGAC TCTAACATTG GTGGTCGTTT TTCTACGCTG AGCCCTGCGG GCCTCCTGTT
      661 GGCTAAGTTG TTTGGTCATG ATCCGAAAGC GATTCTGAAA GGCACCCTGC AAGCGAAGAA
      721 AGATCTGCAA ACGACCAGCC TGGAGAATAA TAGCGCGTAT CTGTACGCAG TCGTTCGCCA
      781 CTGGCTGTAC ACCACGAAAA AATTCAAAAT CGAAGTGTGT ATCGCGTATC ACAGCCTGTA
      841 TGAGTATCTG CTGCTGCAGC ACCGTCAGTT ATTCGGTGAG TCCGAGGGTA AGAATGACAA
      901 ATCCCTGTTC CCGACGTTCT CTATCTTCAC GGTTGATTTA CACAGCATGG GTCAACTGTA
      961 CCAGGAAGGC GAAAAAGTGT TCTTCGAAAC GGTGATTGAC GTTAAAAATC CGCTGGTTAA
     1021 CATTAATCTG CCGCCGAGCG ACTTTGATAA TGACGACGAG CTTGATTTCC TGCTGGACAA
     1081 GAGCTTGAAT GAAATTTCGG ATGTGGCGAT TGACAGCGTG ATCAAGGCAC ACTACCAGGC
     1141 TAACGTTAGC ATTATTAAAT TAACCCTGAA GGAGCAAAGC GCTTTCATGT TTGGCTATTT
     1201 TTACTTCTGG CTGTCGGTCG CGACCGTCAT GAGCGGTAGC CTGCTGGGCC ACAACGTGTT
     1261 TAACCAACCG GGCGTCGAAG TGTATAAGAA GCTGATGTTC GAAAAGCTGC GCAGCGGCCA
     1321 TTGAAGAGCT TAGAGACCCT ATTCGGTCTG CTCGTTGTCG CTGAGGTGTC AATCGTCGGA
     1381 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1441 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1501 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1561 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1621 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1681 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1741 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1801 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1861 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1921 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1981 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     2041 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2101 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     2161 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2221 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2281 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2341 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2401 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2461 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2521 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2581 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2641 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2701 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2761 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2821 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2881 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2941 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     3001 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     3061 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     3121 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3181 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3241 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3301 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//