LOCUS       Exported     2920 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2920
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      958..1005
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2853..2869
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2870..2901
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..886
                     /label="MG_RS00680"
                     /note=""
                     /translation="MIDLLGLDLDGTLLSKTKKINNPSKLALTNLIAKKPSLKVMILTGRSVFSTLKHVEKLNSLFKKPIVDYFCCYGGAKLYQIEANKPQERYKFCLENSVVETTFSIIKKHRGLCLAYLDSYVSPYLCLAGNKLLGWFTKYFWYRKRCVFFNQNHLKQGILKISVYFLSAKRCKKVYEILKNTFQEKVNVLSFSNNLIEITHHDANKGYAIEYMAKREQLSLNRIAVIGDSWNDYAMFKKAKYSFAMSKSPSQLKLIATNTSNKTNRYRFSTLLNLISETIINQKAD"
     primer_bind     complement(1010..1026)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1093..1681)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1872..2732)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2733..2837)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GATTGACTTG CTGGGTTTGG ATCTGGACGG
       61 CACCCTGCTG AGCAAGACTA AAAAGATCAA CAACCCTAGC AAACTCGCGT TGACCAACCT
      121 GATCGCGAAA AAGCCGTCGC TTAAGGTTAT GATCCTGACC GGCCGTAGCG TGTTTTCGAC
      181 CCTGAAGCAT GTCGAAAAAC TCAACAGCCT GTTCAAGAAA CCGATCGTCG ATTACTTCTG
      241 CTGCTATGGC GGCGCGAAAT TGTACCAGAT CGAAGCGAAT AAACCGCAGG AGCGCTATAA
      301 GTTCTGTCTG GAGAATTCTG TGGTGGAGAC TACGTTCAGC ATCATCAAAA AGCACCGTGG
      361 TCTGTGTCTG GCTTACTTGG ATTCCTATGT TAGCCCTTAC CTGTGTTTGG CGGGTAACAA
      421 GTTGCTGGGT TGGTTTACCA AATATTTCTG GTACCGTAAA CGTTGTGTGT TCTTCAATCA
      481 GAATCACCTG AAGCAGGGCA TCCTGAAGAT TAGCGTTTAC TTCCTGTCCG CGAAGCGTTG
      541 TAAAAAGGTC TACGAGATTC TGAAGAACAC CTTTCAAGAA AAAGTTAATG TCCTGTCCTT
      601 CAGCAACAAC CTGATTGAGA TTACCCACCA TGACGCTAAT AAGGGTTACG CGATTGAGTA
      661 CATGGCGAAA CGTGAGCAAC TGAGCTTGAA TCGTATTGCA GTCATTGGTG ACTCCTGGAA
      721 CGACTATGCA ATGTTTAAGA AGGCAAAATA CAGCTTTGCA ATGAGCAAAT CTCCAAGCCA
      781 ACTGAAACTG ATTGCGACGA ATACGAGCAA CAAAACGAAT CGCTATCGCT TTAGCACTCT
      841 GTTGAACCTG ATCTCCGAAA CCATCATTAA CCAGAAGGCG GATTGAAGAG CTTAGAGACC
      901 GTTGTATCCT TCTGGGCCTT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
      961 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1021 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1081 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1141 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1201 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1261 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1321 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1381 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1441 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1501 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1561 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1621 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1681 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1741 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     1801 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     1861 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     1921 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     1981 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2041 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2101 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2161 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2221 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2281 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2341 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2401 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2461 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     2521 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     2581 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     2641 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     2701 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     2761 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     2821 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     2881 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//