LOCUS       Exported     3106 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3106
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1144..1191
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3039..3055
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3056..3087
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1072
                     /label="MG_RS00685"
                     /note="Catalyzes the attachment of tryptophan to tRNA(Trp)."
                     /translation="MIKRAITGIQASGRQHLGNFLGVMQGLKQLQSQYQLFLFVADLHAITVDFEPTMLKDNNLQLVKTLLALGLDYGKVNLFLQSDLMEHTMLGYLMLTQSNLGELQRMTQFKTKKLAQKRNSNNTITIPTGLLTYPVLMAADILLYQPDIVPVGNDQKQHLELTNDLAKRVAKKFKLKLKLPVFIENKDTNRIMDLSNPLKKMSKSNPDQNGVIYLDDSKETIIKKVRKATTDSFNKIRFAKKTQPGVTNLLVILTALLKEEVNHNLSKKIGSDLVKYYQNKSYLDLKNDLSSAVINVIESLKFKKAQITDEMVLKVLNDGKNQAKKVADETLKMFYKAFGLTSNQLFD"
     primer_bind     complement(1196..1212)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1279..1867)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2058..2918)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2919..3023)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GATTAAACGC GCGATTACCG GTATTCAGGC
       61 GTCCGGTCGC CAACACCTCG GTAATTTTCT GGGTGTGATG CAGGGTCTGA AGCAACTGCA
      121 AAGCCAATAT CAATTATTTC TGTTCGTCGC GGATCTGCAC GCCATCACGG TGGACTTCGA
      181 GCCGACGATG TTGAAAGATA ACAATCTGCA GCTGGTGAAA ACCCTGCTGG CATTGGGTCT
      241 GGACTATGGC AAGGTTAACC TGTTCCTTCA ATCCGATCTG ATGGAGCATA CGATGCTTGG
      301 CTACCTGATG CTGACGCAAT CCAATTTGGG TGAGCTGCAG CGCATGACCC AGTTTAAAAC
      361 CAAAAAACTG GCACAGAAGC GTAATTCCAA TAATACCATT ACCATCCCGA CCGGTCTGCT
      421 GACGTATCCA GTGCTGATGG CGGCGGATAT TCTGCTGTAT CAGCCGGACA TTGTACCTGT
      481 GGGCAACGAT CAGAAGCAGC ATCTGGAGCT GACGAATGAC CTGGCAAAGC GCGTTGCAAA
      541 GAAATTTAAG CTGAAATTGA AACTGCCGGT TTTCATCGAG AATAAGGATA CCAATCGTAT
      601 CATGGATCTG AGCAACCCGC TGAAAAAGAT GTCAAAAAGC AATCCGGATC AGAATGGTGT
      661 GATCTACCTG GATGATAGCA AAGAAACGAT TATCAAAAAA GTACGTAAGG CGACCACCGA
      721 TAGCTTCAAC AAAATCCGTT TTGCCAAGAA AACCCAACCG GGTGTCACGA ACTTACTGGT
      781 CATCCTGACG GCGCTGCTGA AGGAAGAGGT TAATCATAAC CTGAGCAAAA AGATCGGCTC
      841 TGACCTGGTT AAATACTATC AAAACAAATC GTATCTGGAT CTGAAGAATG ATTTATCTTC
      901 CGCGGTGATC AACGTTATTG AGAGCTTGAA GTTTAAGAAG GCACAAATTA CCGACGAGAT
      961 GGTGCTGAAA GTGCTGAACG ACGGCAAAAA CCAAGCCAAG AAAGTTGCGG ATGAAACCCT
     1021 GAAAATGTTC TACAAAGCAT TCGGCCTGAC TTCGAATCAA CTGTTCGATT GAAGAGCTTA
     1081 GAGACCCGGC TGGCTGTAAG AATACTCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     1141 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1201 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1261 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1321 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1381 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1441 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1501 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1561 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1621 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1681 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1741 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1801 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1861 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     1921 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     1981 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     2041 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     2101 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     2161 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     2221 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     2281 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     2341 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     2401 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     2461 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     2521 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     2581 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     2641 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     2701 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     2761 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     2821 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     2881 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     2941 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     3001 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     3061 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGGGAG
//