LOCUS       Exported     2857 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2857
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      895..942
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2790..2806
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2807..2838
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..823
                     /label="MG_RS00725"
                     /note=""
                     /translation="MLKKSIGIFYRCFYLNNKCDYYLIFLAPFSLFTQIFMVITALISVANSGQMSLIWFTNFDTFTYQSNSLAIFLVWYYFLNHKSRWFENSSLVLSVTGYLVFTVIFFNFYALSRFTGIVNIEPDVQGWFSTITTQLPYSFNGSFINDWNAFSELLLHVIHPLFYFIYVGLLFKTYKFIKPPRNLQSFLLKAGIYPSIYAFYLQTIPFLNVWDNGENSYSVYGFFTQTKYNSYVWIWSIPIFASMFLILWMLFVINNHYYGKKHHK"
     primer_bind     complement(947..963)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1030..1618)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1809..2669)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2670..2774)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GCTGAAAAAG AGCATCGGCA TCTTCTATCG
       61 TTGCTTCTAC CTGAACAATA AGTGCGATTA CTATCTGATC TTCCTCGCAC CGTTTTCGCT
      121 GTTTACGCAA ATCTTCATGG TCATCACCGC ACTGATTAGC GTCGCAAACA GCGGTCAGAT
      181 GAGCTTGATT TGGTTCACGA ACTTTGATAC GTTCACCTAT CAAAGCAATT CCTTGGCGAT
      241 TTTTCTGGTA TGGTACTATT TTCTGAATCA TAAATCTCGC TGGTTTGAAA ACTCCAGCTT
      301 GGTCCTGTCC GTTACCGGCT ATTTGGTTTT CACGGTAATT TTCTTCAATT TCTACGCGTT
      361 GAGCCGTTTC ACGGGTATCG TTAACATCGA GCCGGATGTT CAAGGCTGGT TTAGCACCAT
      421 CACCACCCAA CTGCCGTATA GCTTTAATGG CAGCTTCATT AACGATTGGA ACGCATTCAG
      481 CGAGCTGCTG CTGCACGTGA TTCACCCGCT GTTTTACTTC ATTTATGTTG GTCTGCTGTT
      541 CAAGACCTAC AAATTCATCA AACCGCCGCG TAATCTGCAG TCTTTCCTGC TCAAGGCGGG
      601 CATTTATCCG TCCATTTACG CATTCTATCT GCAAACTATT CCGTTCCTGA ATGTTTGGGA
      661 CAACGGTGAG AATAGCTACT CTGTGTATGG CTTTTTTACT CAAACCAAAT ACAACAGCTA
      721 TGTGTGGATC TGGAGCATCC CGATCTTCGC GTCCATGTTT CTGATCCTGT GGATGTTGTT
      781 TGTGATCAAT AACCACTATT ATGGCAAAAA GCATCACAAG TGAAGAGCTT AGAGACCGGC
      841 GGTGTCTACA TATCCTTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
      901 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
      961 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1021 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1081 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1141 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1201 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1261 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1321 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1381 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1441 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1501 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1561 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1621 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1681 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1741 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     1801 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     1861 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     1921 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     1981 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2041 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2101 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2161 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2221 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2281 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2341 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2401 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2461 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2521 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2581 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2641 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2701 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     2761 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     2821 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//