LOCUS       Exported     3292 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3292
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1330..1377
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3225..3241
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3242..3273
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1258
                     /translation="MKYVKVQIINKSTIELLEDAKKGEKINLDLINQVDQTNILNTITTNQKLAWEKELSAQFINQQNELIKNFEIEIIKLKTMLNDKEQALLLKTKLELQNQFQKQIENYINEINKLKLTNKELEITNQKQLESSLKLQRNEFEEKINQQNLTIEKLKIQQARSSIWAVAKKGNELEKWCENQYESYADSFENCQFTRYKTEINLLDENDFPNEKADYIFSFFGEKTNKIPFLSICCEMKSEFNDSKHKSKNKDHISKLVRDAKRANCKYAFLISELELETENDIQVRLMPTLESGVEVYLVRPMFFILMLKLFYKLAKKLFALNRFQSVELIDKNKLNEQFKQLKDNFLTKTFLEIEKVCKSNLVDIETLEKAVVKLRVRNERVLDQLLNKWTKKIDSFDLQLTKKITNNY"
                     /label="MG_RS00845"
                     /note=""
     primer_bind     complement(1382..1398)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1465..2053)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2244..3104)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3105..3209)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GAAATATGTT AAAGTTCAGA TCATTAACAA
       61 GAGCACCATT GAACTGCTGG AGGACGCGAA AAAGGGCGAG AAGATTAACC TGGATCTGAT
      121 CAATCAGGTG GACCAGACGA ATATCCTGAA TACCATCACG ACCAATCAGA AGCTGGCCTG
      181 GGAGAAGGAA TTGTCAGCCC AATTTATCAA TCAGCAGAAT GAACTGATTA AAAATTTTGA
      241 AATCGAAATT ATTAAGCTGA AAACCATGCT GAATGACAAA GAACAGGCAC TCCTCCTGAA
      301 AACCAAACTG GAGCTGCAAA ACCAATTCCA AAAGCAGATC GAGAATTACA TCAATGAGAT
      361 CAACAAGCTG AAACTGACCA ACAAAGAACT GGAAATTACG AACCAAAAGC AGCTGGAAAG
      421 CAGCTTGAAA CTGCAGCGCA ACGAATTCGA GGAGAAAATC AATCAGCAAA ACCTGACCAT
      481 TGAGAAACTG AAGATCCAGC AGGCGCGTAG CAGCATCTGG GCGGTTGCCA AGAAGGGCAA
      541 TGAACTGGAG AAATGGTGCG AGAATCAATA CGAAAGCTAC GCGGACAGCT TCGAGAACTG
      601 CCAGTTCACC CGCTACAAGA CGGAGATCAA TCTGCTGGAT GAGAATGACT TCCCGAACGA
      661 AAAGGCGGAC TATATTTTTA GCTTCTTTGG CGAGAAAACG AATAAAATTC CGTTCCTGAG
      721 CATCTGCTGC GAAATGAAAT CTGAGTTTAA CGACAGCAAG CATAAGAGCA AGAATAAAGA
      781 CCACATTAGC AAACTGGTTC GTGATGCGAA GCGTGCAAAT TGTAAATACG CTTTTCTGAT
      841 CAGCGAACTG GAATTAGAAA CCGAAAACGA TATTCAGGTT CGCCTTATGC CGACGCTGGA
      901 GAGCGGTGTT GAGGTCTATC TGGTCCGTCC GATGTTTTTC ATTCTGATGC TGAAACTCTT
      961 TTACAAGCTG GCTAAGAAGC TGTTTGCGCT GAACCGTTTC CAGAGCGTCG AGTTAATTGA
     1021 CAAAAATAAA CTGAACGAAC AGTTTAAGCA GCTGAAAGAC AACTTCTTGA CGAAAACGTT
     1081 TCTGGAGATT GAGAAAGTTT GCAAAAGCAA CTTGGTCGAT ATTGAAACGC TGGAAAAAGC
     1141 CGTCGTGAAA CTGCGTGTCC GCAATGAGCG CGTTCTGGAC CAACTGCTGA ACAAGTGGAC
     1201 CAAGAAAATC GACAGCTTTG ATCTGCAGTT GACTAAGAAA ATCACCAACA ACTACTGAAG
     1261 AGCTTAGAGA CCCGCTAAGA TCCGTAGGTC TGCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1321 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1381 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1441 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1501 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1561 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1621 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1681 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1741 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1801 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1861 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1921 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1981 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2041 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2101 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2161 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2221 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2281 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2341 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2401 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2461 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2521 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2581 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2641 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2701 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2761 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     2821 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     2881 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     2941 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3001 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3061 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3121 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3181 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3241 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//