LOCUS       Exported     2836 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2836
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      874..921
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2769..2785
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2786..2817
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..802
                     /label="MG_RS00865"
                     /note="One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit."
                     /translation="MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTAKKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK"
     primer_bind     complement(926..942)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1009..1597)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1788..2648)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2649..2753)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GGATGTCCGC GGTATCTTTG GTGTGAAGGT
       61 GGGTATGTCC CAAATTTTCA CCGAACAGAA CGAATGCCTG CCGATTACGA TCGTTTATTG
      121 CGAGGCAAAT CAGGTGGCGG GTATTAAAAC GATTGCGAAG GATAACTACA ACGCAACCCT
      181 GCTGAGCTTC CAGACCGTTG ATGAAAAACA GCTGAACAAA CCGAAACAAG GTTTTTTCAG
      241 CAAGCTGAAA CTGGAACCGC ACAAATATCT GCGTGAAATC CGTAAGATGC AAGGTTTCGA
      301 ACTGGGTAAG AAGATTACCC CGCAAGAGCT GTTCAAGATT GGTGAGTATG TGGACGTGAC
      361 GAGCCTGACC AAGGGTCGTG GCTTTACGGG TGCAATCAAG CGTTGGAACT TTAAGATCGG
      421 TCCGCTGGGC CATGGCGCGG GCTATCCGCA CCGCTTCCAG GGCTCTGTCC AGGCAGGCCG
      481 TGGTGGTAGC AGCGCCCAGC GCGTGTTTAA AGGTAAAAAG ATGAGCGGCC ACTATGGTCA
      541 TGAACAAGTC ACCATCCAGA ATCTGTTTAT CGTCGGCTTT GACGAGATCA ATAAGCTGGT
      601 CTTGGTTAGC GGCGCGATCG CTGGTCCGGA AGGTGGCATC GTTCTGATCA AGACCGCCAA
      661 GAAAAAGACC GGCAAAATCA AGGACATTAA GCTGGCCGTT CAGACCGTGA AAGCACCTCA
      721 GCTGAAAGCA CCGAAGAAGC AGAAAACCAA GGTCGAAACG AACCAGGTCA ACCCGAAGAT
      781 CGAGGAGGAA AAGACCAAGT GAAGAGCTTA GAGACCCGCA TGTAGGCACC ATCTATCGCT
      841 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      901 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
      961 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1021 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1081 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1141 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1201 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1261 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1321 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1381 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1441 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1501 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1561 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1621 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1681 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1741 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1801 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     1861 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     1921 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     1981 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2041 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2101 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2161 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2221 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2281 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2341 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2401 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2461 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2521 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2581 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2641 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2701 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2761 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     2821 TCAGGTGGAG TGGGAG
//