LOCUS Exported 2653 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2653 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 691..738 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2586..2602 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2603..2634 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..619 /label="MG_RS01175" /note="" /translation="MFFLSKYKLFLDCAYKTLNIIILEMKTNAVVDELSIGVEQNLTELAVYYLETMLTKNKLKKSSIKQFYVTIGPGSFTGQRIATIIAKSWCLLYPSCELYALNSLRFQIPYEHGISKISCGNDQNYCGLYSQTTSEIKLISKADFVKLCKANNELPMYENFENIESYSKLLLSNIDHFERIEDPLTLQPIYLKDPVN" primer_bind complement(743..759) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(826..1414) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1605..2465) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2466..2570) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GTTTTTTCTG AGCAAATATA AATTGTTTCT 61 TGACTGTGCT TATAAGACCC TGAACATTAT TATTCTGGAG ATGAAAACCA ACGCAGTTGT 121 TGACGAATTA TCTATCGGCG TTGAGCAAAA CCTGACTGAG CTGGCGGTTT ACTATTTGGA 181 AACGATGCTG ACTAAGAACA AGCTGAAAAA GTCTAGCATC AAACAGTTTT ATGTTACCAT 241 TGGCCCGGGT AGCTTCACGG GTCAGCGCAT TGCCACCATT ATTGCTAAAT CCTGGTGCCT 301 GCTGTATCCG TCTTGCGAGC TGTACGCGTT GAATAGCCTG CGCTTCCAGA TCCCGTACGA 361 ACATGGTATT AGCAAAATCT CGTGTGGCAA TGACCAGAAC TACTGCGGTC TGTACTCCCA 421 AACCACCTCC GAAATTAAAT TGATCAGCAA AGCCGACTTC GTCAAACTGT GTAAGGCTAA 481 TAATGAGTTG CCAATGTATG AGAACTTTGA AAACATTGAG TCCTATAGCA AACTGCTGTT 541 ATCTAACATC GATCACTTCG AACGTATCGA GGATCCGCTG ACCCTGCAGC CGATTTACCT 601 GAAGGACCCG GTGAACTGAA GAGCTTAGAG ACCGACCTTG GGATCGCTTA TGTCGCTGAG 661 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG 721 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA 781 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG 841 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 901 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 961 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 1021 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 1081 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 1141 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 1201 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 1261 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 1321 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 1381 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT 1441 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 1501 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 1561 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 1621 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 1681 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 1741 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 1801 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 1861 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 1921 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 1981 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 2041 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 2101 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 2161 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 2221 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 2281 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 2341 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 2401 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 2461 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 2521 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC 2581 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA 2641 GGTGGAGTGG GAG //