LOCUS Exported 2989 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2989 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1027..1074 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2922..2938 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2939..2970 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..955 /note="" /translation="MKQCFVVTTTKRLDSLLASLLNLSRVKVVKLIMNGQIKVNEKLTFKNSLIVAKDDVIKVEIHDETTSDFITSVEPYNLKLEVLFEDKDLMVINKPSGLLTHPTTFNEKASLLAACIFHNNKNPVYLVHRLDRDTSGAIVVCKNQASLLNLQNQLQNRTLKRYYVALVHFPFNALTGSINAPLARVNNNKVMFKIAQTAKAKQAITKFKVINQNEKAALISLELLTGRTHQIRVHLKFIQHPVYNDPLYGIKSEKKDSYGQFLHANRICFIHPTLNKPMDFHAPLEPKFSTKLKSLNLSLTDPLHVLFK" /label="MG_RS01180" primer_bind complement(1079..1095) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1162..1750) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1941..2801) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2802..2906) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GAAGCAATGT TTCGTTGTCA CCACGACCAA 61 GCGTTTGGAT AGCTTACTGG CCAGCCTGCT GAACCTGAGC CGTGTGAAAG TGGTTAAGCT 121 GATCATGAAC GGTCAGATTA AGGTGAATGA AAAGCTGACC TTTAAAAACA GCCTGATTGT 181 CGCTAAGGAC GACGTTATCA AAGTTGAGAT CCACGACGAA ACGACGAGCG ATTTCATTAC 241 CAGCGTTGAA CCGTATAACC TGAAACTGGA AGTTCTGTTC GAGGACAAAG ATTTGATGGT 301 CATTAATAAA CCGAGCGGTC TGCTGACCCA TCCGACCACG TTTAACGAGA AGGCAAGCCT 361 GCTGGCGGCA TGCATCTTCC ACAACAATAA AAACCCTGTG TATTTGGTCC ATCGTCTGGA 421 CCGCGACACC TCTGGCGCCA TTGTTGTTTG CAAGAATCAG GCTAGCCTGC TGAATCTGCA 481 GAACCAACTG CAAAACCGCA CCCTGAAACG TTACTACGTT GCGCTCGTCC ACTTTCCGTT 541 TAATGCCCTG ACCGGCAGCA TTAATGCTCC GCTGGCACGC GTTAACAATA ACAAAGTTAT 601 GTTCAAAATT GCACAGACGG CGAAGGCGAA GCAAGCTATC ACGAAATTTA AAGTCATTAA 661 TCAGAATGAG AAGGCTGCTT TGATCAGCCT GGAGCTGCTG ACCGGTCGTA CTCACCAAAT 721 TCGTGTTCAC CTGAAATTCA TTCAGCACCC GGTCTATAAT GATCCGCTGT ACGGTATTAA 781 GAGCGAAAAG AAGGACAGCT ATGGTCAGTT TCTGCACGCA AATCGTATCT GTTTCATCCA 841 CCCGACCCTG AATAAGCCGA TGGACTTTCA CGCACCACTG GAGCCGAAGT TTAGCACCAA 901 ACTGAAAAGC CTGAATCTGT CTCTGACCGA TCCGCTGCAC GTGCTGTTTA AGTGAAGAGC 961 TTAGAGACCC GGATTCTAGG TGCAACTCTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG 1021 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT 1081 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG 1141 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 1201 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 1261 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 1321 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 1381 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 1441 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 1501 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 1561 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 1621 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 1681 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 1741 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT 1801 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 1861 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 1921 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 1981 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 2041 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT 2101 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 2161 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 2221 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 2281 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 2341 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 2401 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 2461 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 2521 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 2581 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 2641 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 2701 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 2761 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT 2821 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 2881 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG 2941 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG //