LOCUS       Exported     2869 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2869
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      907..954
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2802..2818
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2819..2850
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..835
                     /label="MG_RS01210"
                     /note="Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position."
                     /translation="MDKLFKTSFRFIIRFLQILSLPVVFPYFLLSFLACLITSKNYESLPYNYPPEIRFKKVYRLVSMWLYIKGIKVVTVNDKIIPKKPVLVVANHKSNLDPLVLIKAFGRLKNSPPLTFVAKIELKDTVLFKLMKLIDCVFIDRKNIRQIANALETQQQLIRQGTAIAVFAEGTRILSNDIGEFKPGALKVAYNAFVPILPVSIVGSLGKMESNKRLKEHGVKKSSNYEVKVIFNKLINPISFNQIDSNNLANNIRSIISDAYTSEKPSND"
     primer_bind     complement(959..975)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1042..1630)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1821..2681)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2682..2786)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GGACAAGCTG TTCAAGACCA GCTTTCGCTT
       61 CATTATCCGT TTTCTGCAAA TTCTGTCCCT TCCGGTTGTT TTCCCATATT TTCTGCTCAG
      121 CTTTCTGGCG TGTCTGATTA CCAGCAAAAA TTATGAAAGC CTGCCGTACA ACTACCCTCC
      181 GGAAATTCGT TTTAAGAAAG TCTACCGTCT GGTATCCATG TGGCTGTATA TTAAAGGCAT
      241 TAAAGTCGTT ACCGTTAACG ACAAGATCAT TCCGAAGAAA CCAGTGCTGG TTGTGGCGAA
      301 TCACAAGTCT AATCTGGACC CGCTGGTTCT GATTAAGGCG TTTGGTCGTC TGAAGAACTC
      361 GCCGCCTCTG ACCTTTGTGG CGAAAATTGA ACTGAAGGAT ACCGTTCTGT TCAAGCTGAT
      421 GAAGCTGATT GACTGCGTCT TTATCGATCG TAAGAACATT CGCCAGATTG CGAACGCCCT
      481 GGAAACGCAA CAGCAGCTGA TCCGCCAAGG TACTGCTATC GCTGTCTTTG CCGAGGGTAC
      541 CCGCATCCTT TCTAATGACA TTGGCGAGTT CAAACCAGGT GCACTGAAGG TGGCCTATAA
      601 CGCGTTCGTT CCGATCCTGC CGGTTAGCAT CGTGGGTAGC CTGGGTAAAA TGGAAAGCAA
      661 TAAACGTTTG AAGGAGCATG GTGTTAAAAA GTCGAGCAAC TATGAGGTGA AAGTCATTTT
      721 TAACAAGCTG ATCAACCCGA TTAGCTTCAA CCAAATCGAC AGCAACAACC TGGCAAACAA
      781 CATTCGCAGC ATCATTTCGG ATGCGTACAC GTCTGAGAAG CCGAGCAATG ATTGAAGAGC
      841 TTAGAGACCG GGTGCCTTGC TAATCTAGTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
      901 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
      961 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1021 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1081 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1141 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1201 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1261 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1321 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1381 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1441 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1501 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1561 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1621 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1681 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     1741 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     1801 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     1861 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     1921 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     1981 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2041 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2101 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2161 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2221 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2281 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2341 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2401 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2461 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     2521 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2581 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2641 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     2701 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     2761 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     2821 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//