LOCUS       Exported     2938 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2938
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      976..1023
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2871..2887
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2888..2919
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..904
                     /note="Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate."
                     /translation="MPKLLGSFISFKAPNYFVQSAQDAIAIDATALMVFLGPPHSAYRVPFNKMQFSLGYELLKTKNINSNGLVVHAPYIINCASKDPLKQQNAISVLTNEIQLCNLAGAHYLVLHPGSAVAQTTNEALDNLVKVLNQVINKTKTTVICLETMAGKGNEIGRDLTELKYVIDRIVDKDRIGVCLDTCHFHDSGIDFSDLTGVFNTITTKLGFEFLKVIHLNESKNNCGSKKDRHANINAGMIGFENLMKFISHPQIKDLPIILETPSTSLNYPTIYREEISQIRSWFKTYQPDAN"
                     /label="MG_RS01380"
     primer_bind     complement(1028..1044)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1111..1699)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1890..2750)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2751..2855)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GCCGAAATTG CTGGGTAGCT TTATCAGCTT
       61 TAAGGCGCCG AACTATTTTG TTCAGTCCGC CCAGGATGCA ATCGCAATCG ATGCAACGGC
      121 ACTGATGGTG TTCCTGGGTC CGCCTCATAG CGCGTATCGT GTGCCGTTCA ATAAAATGCA
      181 ATTCAGCTTG GGTTATGAGC TGCTCAAGAC CAAAAATATT AACTCGAATG GTCTGGTCGT
      241 GCACGCACCG TACATCATCA ACTGTGCCAG CAAGGATCCG CTGAAGCAGC AGAACGCAAT
      301 CTCCGTTCTG ACGAACGAAA TTCAACTGTG TAATCTGGCG GGTGCGCATT ATCTGGTCCT
      361 GCATCCGGGC AGCGCAGTCG CGCAGACGAC CAACGAGGCG TTGGACAACC TGGTTAAGGT
      421 TCTGAATCAA GTGATCAACA AGACCAAGAC CACCGTGATT TGCTTGGAAA CCATGGCGGG
      481 TAAGGGTAAC GAGATTGGTC GTGATCTGAC GGAGCTGAAG TACGTTATCG ATCGCATTGT
      541 CGACAAGGAC CGTATTGGCG TTTGCCTGGA CACCTGTCAC TTTCACGACA GCGGCATTGA
      601 TTTTAGCGAT CTGACGGGTG TCTTTAATAC CATCACCACC AAGCTTGGTT TTGAATTTCT
      661 GAAAGTCATT CACCTGAATG AAAGCAAAAA CAATTGCGGT AGCAAAAAGG ATCGTCACGC
      721 AAACATCAAC GCCGGTATGA TTGGTTTTGA GAACCTGATG AAATTTATCA GCCACCCGCA
      781 AATCAAGGAT CTGCCGATCA TCCTGGAAAC CCCGAGCACG TCCCTGAATT ATCCGACCAT
      841 TTATCGTGAG GAGATCAGCC AAATCCGTTC TTGGTTCAAA ACTTACCAAC CGGATGCGAA
      901 CTGAAGAGCT TAGAGACCGG CGGTGTCTAC ATATCCTTCG CTGAGGTGTC AATCGTCGGA
      961 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1021 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1081 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1141 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1201 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1261 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1321 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1381 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1441 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1501 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1561 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1621 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1681 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1741 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1801 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1861 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1921 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     1981 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2041 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2101 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2161 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2221 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2281 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2341 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2401 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2461 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2521 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2581 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2641 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2701 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2761 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2821 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2881 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//