LOCUS       Exported     3556 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3556
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1594..1641
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3489..3505
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3506..3537
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1522
                     /label="MG_RS01450"
                     /note="Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth."
                     /translation="MSTDKKTLGEKPNSTKPELSEELIAELKKQRILEKNRPYKKMIYVDNKVQRKHRHENIAFLKTLHENKESDVPKKRRGRKPKHAPLKEKNNLKLFDILEGSLKSHIENDDTNTVINLLTEAWEKKSKKKQKNITLSNKEIISVLAKFELPEDEIIYVLDELRDKGIQLQHDVEEHIHEFRANQDLSIIDEDIEELTSKNISNRDKVDDNVRFFLGSLDFSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFNWSLGNKFSTYATWWIKQAITRAIADQARTVRIPVHMVETINRLAKAERALYQELGREPTDEELAEKMGGQAEGFNVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVKDTDAQTPDEFTESRSNSEKIDELLNNNLSEQEELIVRMRIGMPPYNEPKTLDEVGQKILIPREKIRQIENKAIRKLRHAVRNNPISMSFLRINEKKD"
     primer_bind     complement(1646..1662)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1729..2317)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2508..3368)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3369..3473)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GAGCACCGAC AAAAAGACTT TGGGCGAAAA
       61 GCCGAATAGC ACCAAGCCGG AGCTGAGCGA AGAACTGATT GCGGAGCTGA AAAAACAGCG
      121 TATTCTGGAG AAAAACCGCC CGTATAAGAA AATGATTTAT GTTGACAACA AGGTTCAACG
      181 TAAGCACCGC CATGAGAATA TTGCGTTCCT CAAGACGCTG CACGAAAACA AAGAAAGCGA
      241 CGTTCCGAAA AAACGTCGTG GTCGCAAGCC GAAGCATGCG CCGCTGAAAG AGAAAAATAA
      301 TCTGAAACTG TTTGATATCC TGGAGGGTTC CCTGAAAAGC CACATCGAAA ATGATGATAC
      361 CAACACGGTT ATTAACTTAC TGACCGAAGC ATGGGAGAAG AAAAGCAAGA AAAAGCAAAA
      421 GAATATTACC CTGTCTAACA AAGAAATTAT CAGCGTTCTG GCGAAGTTTG AACTGCCGGA
      481 GGACGAAATT ATTTACGTTC TGGATGAGCT GCGTGATAAA GGCATTCAGC TGCAACACGA
      541 CGTCGAGGAG CATATCCATG AGTTCCGTGC AAATCAAGAC CTGAGCATTA TCGATGAAGA
      601 TATTGAGGAA CTGACCAGCA AGAATATTAG CAATCGCGAT AAGGTCGATG ACAACGTACG
      661 CTTTTTTCTG GGCTCCCTGG ATTTCAGCAA AATGTTGGAT TTTGAATCCG AGCAGCGTAT
      721 TGCGAAGGTT CTGAATTCCA CGGATGAAGA AAGCCGTAAG TACGCGATCA ATCAACTGGT
      781 GACGTCGAAC TTACGTCTGG TCGTAAGCAT TGCCAAGAAG CATCTGGAGC GTGGTCTGGA
      841 CTTCAACGAT TTGATCCAGG AGGGCAATCT GGGTTTGTTA AAGGCGATTA GCAAGTTTAA
      901 TTGGAGCCTG GGTAACAAAT TCTCGACCTA CGCAACTTGG TGGATCAAGC AAGCCATTAC
      961 GCGCGCAATC GCCGACCAGG CGCGCACCGT TCGTATTCCG GTGCACATGG TGGAAACCAT
     1021 CAATCGCCTC GCGAAAGCGG AGCGTGCGCT GTATCAGGAA TTGGGTCGTG AGCCGACCGA
     1081 TGAAGAACTG GCCGAGAAGA TGGGTGGCCA AGCAGAAGGT TTTAACGTCA AGAAAATTGC
     1141 GGAGATTAAG CGTCTGAGCT TGGATCCGGT TTCTCTGGAC AAAACCGTCG GCCACGATGA
     1201 AGAAAGCCAA TTCGGTGACT TCGTAAAAGA TACGGATGCT CAAACGCCGG ACGAGTTTAC
     1261 TGAGAGCCGC TCTAATTCCG AGAAGATCGA TGAACTGCTG AACAACAACC TGAGCGAGCA
     1321 GGAGGAGCTG ATCGTCCGTA TGCGTATTGG CATGCCGCCG TACAACGAAC CGAAAACTCT
     1381 GGATGAGGTT GGTCAAAAAA TCCTGATTCC GCGTGAAAAA ATTCGCCAAA TCGAGAATAA
     1441 AGCGATTCGC AAACTGCGCC ACGCAGTTCG TAACAATCCG ATCAGCATGT CCTTTCTGCG
     1501 TATTAACGAG AAAAAAGATT GAAGAGCTTA GAGACCCGCG GAAAATGGAA AACACTCGCT
     1561 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1621 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1681 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1741 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1801 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1861 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1921 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1981 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     2041 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     2101 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2161 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2221 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2281 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2341 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2401 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2461 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2521 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2581 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2641 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2701 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2761 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2821 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2881 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2941 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     3001 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     3061 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3121 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3181 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3241 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3301 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3361 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3421 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3481 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3541 TCAGGTGGAG TGGGAG
//