LOCUS       Exported     3373 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3373
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1411..1458
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3306..3322
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3323..3354
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1339
                     /label="MG_RS01470"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
                     /translation="MKQFEHRFMIVDSVSQKPTTLVQKTINIYLCGPTVYNDLHLGNTRPLIVFDVLNRVLKKAKYTVNFVQNITDIDDKIIKIAQQQEVSESVVTKQQITAYKSLLKKLNILPIKHIQITEKIDKIPDYIDQLVNQNHAYVSTQNNVYFAVNSLKQYGYLANRMVHLEETDTDKKNKLDFVLWKITTAGIKWNSKWGLGRPGWHVECAFLIDYCFKNELTIHGGGVDLKFPHHENENALHMALYNQPITKHWMHIGHLMIENQKMSKSLQNFLLAVDFLNFHDFRVLRWIFYQKHYLHPIDLNQSLIEKANNDIQRIAKTLNVARTWLVYSEQSELISPKQYDPVFSALLDNLNFANAVAAIWKLIKKINTSIKTKDFSVLREQLSFLEWSIDLLGISFKSIHTKLNVRLIKEWSILHKQKAMDKADQIRKKLIKKMLL"
     primer_bind     complement(1463..1479)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1546..2134)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2325..3185)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3186..3290)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GAAGCAGTTT GAACATCGTT TCATGATCGT
       61 TGACTCTGTC TCCCAAAAGC CGACCACCTT GGTTCAAAAG ACCATTAATA TCTATTTGTG
      121 CGGCCCGACC GTCTACAACG ATCTGCATCT GGGTAACACC CGCCCGCTGA TCGTGTTTGA
      181 CGTCCTGAAC CGTGTTCTGA AGAAGGCGAA GTACACCGTT AACTTTGTCC AAAACATCAC
      241 CGACATCGAT GACAAAATCA TTAAAATTGC GCAACAGCAA GAAGTTAGCG AAAGCGTTGT
      301 GACTAAACAG CAAATCACGG CGTACAAGAG CCTGCTGAAA AAACTGAACA TTCTGCCGAT
      361 TAAACACATC CAGATTACCG AAAAGATTGA TAAGATCCCG GATTATATTG ACCAACTGGT
      421 CAATCAGAAT CACGCGTATG TCTCTACCCA GAACAATGTG TACTTTGCCG TCAATAGCCT
      481 GAAACAATAC GGCTATCTGG CAAATCGTAT GGTGCACCTG GAAGAAACCG ACACCGACAA
      541 GAAAAATAAG CTCGATTTCG TGCTGTGGAA GATTACCACG GCTGGTATCA AATGGAACAG
      601 CAAGTGGGGT TTAGGTCGTC CGGGTTGGCA CGTGGAATGT GCGTTTCTGA TCGATTATTG
      661 CTTTAAAAAT GAACTGACCA TTCATGGTGG TGGCGTGGAC CTGAAGTTTC CTCACCACGA
      721 GAACGAGAAT GCCCTCCACA TGGCGCTGTA CAACCAGCCG ATTACCAAGC ACTGGATGCA
      781 CATTGGCCAC TTGATGATCG AGAACCAGAA AATGAGCAAA AGCCTGCAGA ATTTTCTGCT
      841 GGCGGTTGAC TTCCTGAACT TCCATGATTT TCGTGTGCTG CGTTGGATTT TCTACCAAAA
      901 ACACTATCTG CATCCGATCG ACCTGAACCA GAGCTTGATC GAGAAGGCGA ACAACGACAT
      961 CCAACGCATT GCTAAAACCT TGAATGTGGC CCGTACCTGG CTTGTTTATA GCGAACAAAG
     1021 CGAACTGATC AGCCCTAAAC AATACGACCC GGTTTTCTCT GCACTGCTGG ATAATCTGAA
     1081 CTTTGCGAAT GCTGTAGCAG CCATTTGGAA GCTGATTAAG AAAATTAATA CCAGCATCAA
     1141 AACGAAAGAT TTCAGCGTGT TACGCGAGCA GCTGAGCTTC CTGGAGTGGT CTATCGATCT
     1201 GCTGGGTATT AGCTTCAAGA GCATTCACAC CAAACTGAAC GTGAGACTGA TCAAGGAATG
     1261 GTCAATCTTG CATAAGCAAA AGGCTATGGA CAAGGCTGAC CAAATTCGTA AAAAACTGAT
     1321 CAAAAAGATG CTGCTGTGAA GAGCTTAGAG ACCCCAGCCT CAGATTGTTG TCTCGCTGAG
     1381 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1441 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1501 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1561 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1621 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1681 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1741 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1801 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1861 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1921 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1981 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2041 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2101 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2161 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2221 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2281 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2341 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2401 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2461 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2521 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2581 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2641 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2701 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2761 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2821 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2881 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2941 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     3001 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     3061 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3121 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3181 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3241 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3301 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     3361 GGTGGAGTGG GAG
//