LOCUS Exported 2575 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2575 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 613..660 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2508..2524 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2525..2556 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..541 /label="MG_RS01530" /note="Derived by automated computational analysis usinggene prediction method: Protein Homology." /translation="MITQHLNQQNTRWVFTPGSTPDIWTGAGYRKANNNNNGIPFEQVKPSSSSNTFNPNSDDNKVTPSGGSSKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGTGDQFNKDSEQKWNETDKLGGNLPGFGEVNGASYKIFTYLIIKKSVRFF" primer_bind complement(665..681) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(748..1336) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1527..2387) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2388..2492) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GATCACGCAA CACTTGAATC AGCAGAATAC 61 GCGTTGGGTT TTCACCCCGG GCTCCACGCC GGATATCTGG ACGGGTGCGG GCTACCGTAA 121 GGCCAATAAC AACAACAATG GTATCCCGTT TGAGCAAGTT AAGCCGAGCA GCTCGAGCAA 181 CACGTTTAAT CCGAACAGCG ACGACAATAA GGTCACCCCG AGCGGCGGTA GCAGCAAGCC 241 GACCACCTAC ACGCATCTTC CGAATAGCAT CTCCCCGACC AGCGACTGGA TTAATGCACT 301 GACCTTCACC AACAAAAACA ATCCGCAGCG TAACCAGCTG TTGCTGCGTG CGTTGCTTGG 361 TACTATTCCG GTCCTGATTA ACAAAAGCGG CACGGGTGAT CAATTCAACA AGGACAGCGA 421 GCAGAAATGG AATGAAACCG ACAAGCTGGG CGGTAATCTG CCAGGCTTTG GTGAAGTTAA 481 TGGCGCGTCA TATAAGATCT TTACGTATCT GATCATCAAG AAGTCTGTGC GCTTCTTTTG 541 AAGAGCTTAG AGACCCCGCG TAAGAGGTTC CTATTCGCTG AGGTGTCAAT CGTCGGAGCC 601 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG 661 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT 721 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 781 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 841 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 901 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 961 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 1021 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 1081 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 1141 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 1201 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 1261 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 1321 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG 1381 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 1441 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 1501 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 1561 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 1621 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA 1681 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 1741 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 1801 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 1861 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 1921 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 1981 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 2041 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 2101 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 2161 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 2221 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 2281 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 2341 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 2401 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 2461 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC 2521 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG //