LOCUS       Exported     2659 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2659
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      697..744
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2592..2608
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2609..2640
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..625
                     /note="Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A."
                     /translation="MLIAIVGKPGVGKTSLLQYLKDNYHFSVFYADSFIHEQYQKNNPGYQLIMDHFGKEFVNQTEVDRKKLANYVFSDDKLIEKLSLVTKPLLIAWIKSLKTQFQKKLALIEIAVMLNYWNEYRSLFDYVIKLERDDQLVNLALQQRNSHKKVKDLIKEPNCKIDTIFNNDSIATAALKLIKLLETFLERNKCRCDCCHIQ"
                     /label="MG_RS01570"
     primer_bind     complement(749..765)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(832..1420)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1611..2471)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2472..2576)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GCTGATCGCG ATCGTCGGTA AGCCGGGTGT
       61 AGGCAAAACG AGCCTGCTGC AATACCTGAA GGACAATTAC CACTTCTCTG TGTTCTATGC
      121 AGACAGCTTT ATCCACGAAC AATACCAGAA AAATAACCCA GGTTACCAAC TGATTATGGA
      181 CCACTTTGGT AAAGAATTCG TAAATCAAAC GGAGGTTGAT CGTAAGAAAC TGGCGAACTA
      241 CGTGTTTTCT GATGACAAGC TGATCGAGAA ACTGAGCCTG GTTACGAAAC CGCTGCTGAT
      301 TGCTTGGATT AAGAGCCTTA AAACCCAGTT CCAGAAAAAG TTAGCGCTGA TCGAGATTGC
      361 CGTTATGCTG AATTACTGGA ACGAGTACCG TAGCCTCTTT GATTATGTTA TCAAACTGGA
      421 GCGTGACGAT CAACTGGTGA ATCTGGCGCT GCAGCAGCGT AATTCTCACA AGAAAGTTAA
      481 AGACCTGATC AAAGAGCCGA ATTGCAAGAT CGACACCATC TTCAATAATG ACTCAATCGC
      541 GACCGCCGCA TTAAAACTGA TTAAGCTGCT GGAAACGTTC CTGGAGCGTA ATAAGTGCCG
      601 TTGTGATTGC TGTCATATCC AGTGAAGAGC TTAGAGACCG CCTTCGCCTT TTCTAGGATC
      661 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
      721 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
      781 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
      841 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
      901 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
      961 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1021 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1081 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1141 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1201 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1261 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1321 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1381 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     1441 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     1501 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     1561 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     1621 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     1681 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     1741 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     1801 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     1861 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     1921 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     1981 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2041 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2101 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2161 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     2221 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     2281 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     2341 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     2401 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     2461 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     2521 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     2581 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     2641 TGGTCAGGTG GAGTGGGAG
//