LOCUS       Exported     3139 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3139
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1177..1224
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3072..3088
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3089..3120
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1105
                     /label="MG_RS01625"
                     /note="The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity)."
                     /translation="MAILIKNKVPTTLYQVYDNEGKLIDPNHKITLTDEQLKHAYYLMNLSRMMDKKMLVWQRAGKMLNFAPNLGEEALQVGMGLGLNENDWVCPTFRSGALMLYRGVKPEQLLLYWNGNEKGSQIDAKYKTLPINITIGAQYSHAAGLGYMLHYKKQPNVAVTMIGDGGTAEGEFYEAMNIASIHKWNTVFCINNNQFAISTRTKLESAVSDLSVKAIACGIPRVRVDGNDLIASYEAMQDAANYARGGNGPVLIEFFSYRQGPHTTSDDPSIYRTKQEEEEGMKSDPVKRLRNFLFDRSILNQAQEEEMFSKIEQEIQAAYEKMVLDTPVSVDEVFDYNYQELTPELVEQKQIAKKYFKD"
     primer_bind     complement(1229..1245)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1312..1900)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2091..2951)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2952..3056)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GGCGATCTTG ATTAAGAACA AGGTCCCGAC
       61 CACGCTGTAC CAGGTCTACG ATAATGAAGG TAAGCTGATC GACCCGAACC ACAAGATCAC
      121 GCTGACGGAC GAACAACTGA AACACGCGTA TTACCTGATG AATCTGAGCC GCATGATGGA
      181 TAAGAAAATG CTGGTCTGGC AACGTGCTGG TAAGATGCTG AATTTTGCGC CGAATCTGGG
      241 TGAGGAGGCG TTGCAAGTCG GTATGGGTCT GGGCCTGAAC GAGAATGACT GGGTGTGCCC
      301 GACGTTTCGT AGCGGTGCGC TGATGCTGTA CCGTGGCGTT AAGCCGGAGC AGCTGCTGCT
      361 GTATTGGAAC GGTAATGAGA AGGGTAGCCA GATTGATGCG AAATACAAAA CCCTCCCGAT
      421 CAATATCACG ATCGGCGCAC AGTATAGCCA TGCAGCTGGT CTGGGCTACA TGCTGCACTA
      481 TAAGAAGCAA CCTAATGTAG CTGTTACCAT GATTGGTGAC GGTGGTACCG CGGAAGGCGA
      541 GTTCTATGAG GCAATGAACA TTGCCAGCAT TCATAAATGG AATACTGTTT TCTGCATTAA
      601 TAACAACCAA TTCGCGATTA GCACCCGCAC GAAACTGGAA TCTGCGGTTA GCGACTTGTC
      661 GGTTAAAGCG ATCGCCTGCG GCATCCCGAG AGTTCGTGTC GATGGCAACG ACCTGATTGC
      721 GTCCTACGAG GCTATGCAAG ATGCAGCGAA CTATGCCAGA GGTGGCAACG GCCCTGTTCT
      781 GATCGAGTTT TTCAGCTACC GTCAGGGTCC GCACACTACC TCTGATGATC CGAGCATCTA
      841 TCGTACTAAA CAGGAAGAGG AGGAAGGTAT GAAAAGCGAT CCGGTTAAAC GTTTGCGTAA
      901 CTTTTTGTTC GACCGTTCGA TCCTGAATCA GGCCCAGGAA GAAGAAATGT TTTCCAAAAT
      961 TGAGCAAGAG ATCCAAGCAG CCTACGAAAA GATGGTGCTG GATACCCCGG TTTCCGTGGA
     1021 CGAAGTGTTC GATTACAATT ACCAAGAGCT GACCCCAGAA CTTGTTGAAC AAAAACAGAT
     1081 TGCTAAGAAA TACTTCAAGG ATTGAAGAGC TTAGAGACCG CCATTGGAAA ACTGAACGTC
     1141 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1201 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1261 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1321 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1381 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1441 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1501 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1561 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1621 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1681 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1741 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1801 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1861 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     1921 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     1981 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     2041 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     2101 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2161 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2221 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2281 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2341 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2401 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2461 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2521 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2581 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2641 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     2701 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     2761 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     2821 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     2881 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     2941 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     3001 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     3061 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     3121 TGGTCAGGTG GAGTGGGAG
//