LOCUS       Exported     4975 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4975
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      3013..3060
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4908..4924
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4925..4956
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2941
                     /label="MG_RS01640"
                     /note=""
                     /translation="MRFKKRFSLDWILKIGTILGLVCLGLFGVIFGSYKLLNDSRLGAVFNGSTTTTVYFLNHKSTNNTSLDPQQTKPTNGIENITNIDSFLDGVEKSYANSLFIQGFSSVNITKNTNDKTASELDNIDKSWLVNGGLPSVTLTFEQNREQAKTRQRKRQVDAQVRRNAISSVEHNYQLSLETTDGVVLFDSLDNNFINASIRAVVPQNTSVNSALTFEYKLNKNVVTKESLHTDFLDFINSKNLSSSDYNTGNGQASVEGNGKFFKQNANGTSSSGNKTLVLWKDKQGALNYVRNIFNVVEGSSDYLTFNEREKSLWEFLHAKGSFASGDNLFLNTDANGASPIKKASDITLKNLYYIYAAPNHFSAVASNSNDNNNQNNNNNNNSSDVITVSNSADTRKLRSADSSGFSGLFHNYIISEIRTEDPVSKTGDPVKINPTLQSFLDTNNQRIEYGGNIKFQVGNFLSSDGTYTPPNFVTAATVKELLTNPFPTAATIATAKTSLVNAPLANTITDVSNFASSFIAFGIIVLIAAVLLTLRYKLLGLYKALALGLSVVSSLVIFSAVGGVVDVFSFVGIFFVIAINLINLLNLGELFLRNIRNNASIIESWKLCLKRSFFANLEFHITWLISALVVIYLSNYQVQQLANLMAISAITSYFFSYGISIVLISLFVSSESGANWKLFLYQKDAKALTKTSSNYSLLSSTSDLNTDFFITKNQHDFFLKNNWKFLFVWLILLAIGVVMLVLYLVQPNLLGEFLAADVESSNGIIAGIGIVSLLYLAYCLIRYGVAYCLSYLVSFILLCSGLFAVMYLTNYLFSIDQSTIQLITFVYLFWLFFAAKVSQTTTWTYFYWFKRSLKDKVFIKNLFNDNFNSQWKIDLIESSSLVFIFIIYSGFNFGGIDGNFNLVIFYLIAIVGLFDVATAFLPMFCFGLINGWLSPFNYVHSRLSLKHKKHPFKEIDQIEEQLIAGINSF"
     primer_bind     complement(3065..3081)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(3148..3736)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3927..4787)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4788..4892)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GCGCTTTAAG AAGCGTTTTA GCTTGGACTG
       61 GATTCTGAAA ATTGGCACGA TCTTGGGTCT GGTTTGCCTG GGTCTGTTTG GCGTGATCTT
      121 CGGCAGCTAT AAGTTGTTGA ACGATTCTAG ACTGGGTGCC GTATTTAACG GCAGCACGAC
      181 CACGACGGTA TATTTCCTCA ATCATAAGTC TACGAACAAT ACCAGCTTAG ATCCGCAGCA
      241 GACCAAACCT ACGAACGGTA TTGAGAATAT TACCAATATT GATAGCTTCC TGGACGGTGT
      301 TGAGAAGTCC TACGCAAACA GCCTCTTCAT TCAGGGTTTT AGCAGCGTGA ACATCACGAA
      361 AAATACCAAT GACAAGACCG CGTCGGAGCT GGATAACATC GATAAGAGCT GGCTGGTGAA
      421 CGGTGGCCTG CCGAGCGTTA CCCTGACCTT CGAACAAAAC CGTGAGCAGG CTAAGACCCG
      481 TCAACGTAAG CGTCAGGTTG ACGCCCAGGT TCGCCGTAAT GCGATTAGCT CGGTCGAACA
      541 TAACTACCAA CTGAGCCTGG AAACCACGGA TGGTGTTGTG CTGTTTGACA GCCTGGACAA
      601 CAACTTCATC AATGCGTCCA TTCGCGCGGT TGTGCCACAG AATACCTCCG TGAACTCCGC
      661 TCTGACGTTC GAGTATAAAC TGAACAAAAA CGTCGTTACG AAAGAAAGCC TGCACACCGA
      721 CTTCCTGGAT TTTATTAACT CCAAGAATCT GAGCAGCTCC GACTATAATA CGGGTAATGG
      781 CCAAGCGTCG GTGGAGGGCA ACGGTAAGTT CTTTAAACAG AATGCGAACG GTACCAGCTC
      841 TAGCGGTAAC AAGACGCTGG TACTGTGGAA GGACAAACAG GGTGCGCTGA ACTACGTCCG
      901 TAATATCTTC AACGTAGTGG AGGGCAGCAG CGACTATCTG ACGTTTAACG AACGCGAAAA
      961 GAGCCTGTGG GAATTTCTGC ATGCAAAAGG TAGCTTTGCC AGCGGCGACA ACCTGTTTCT
     1021 GAACACCGAC GCCAATGGTG CATCTCCGAT CAAAAAAGCC AGCGACATCA CCTTGAAGAA
     1081 TTTGTATTAT ATTTACGCCG CCCCGAACCA TTTTAGCGCG GTTGCGAGCA ACAGCAATGA
     1141 CAACAATAAC CAAAACAATA ATAATAACAA TAACAGCTCC GATGTGATCA CCGTTAGCAA
     1201 CTCTGCTGAT ACCAGAAAGC TGCGTTCTGC GGACTCTAGC GGTTTTAGCG GCCTGTTCCA
     1261 CAACTATATT ATCAGCGAGA TTCGCACGGA AGATCCGGTT AGCAAGACCG GCGATCCGGT
     1321 CAAAATTAAT CCGACGCTGC AAAGCTTCTT GGACACCAAT AATCAACGCA TTGAATATGG
     1381 TGGTAACATC AAGTTTCAAG TTGGTAACTT TCTTAGCAGC GACGGCACCT ACACCCCGCC
     1441 AAATTTTGTG ACCGCGGCCA CCGTCAAAGA GCTCCTGACG AACCCGTTCC CGACGGCGGC
     1501 CACGATCGCT ACGGCTAAAA CCAGCCTGGT CAATGCCCCG CTGGCTAACA CCATTACCGA
     1561 TGTTTCTAAC TTCGCGTCGA GCTTTATCGC GTTCGGTATT ATCGTTCTGA TTGCAGCTGT
     1621 TCTGCTGACT CTGCGCTACA AGCTGCTGGG CTTGTACAAG GCACTGGCTC TGGGCCTGAG
     1681 CGTGGTTAGC AGCCTGGTCA TCTTCAGCGC TGTGGGTGGT GTCGTGGACG TGTTCAGCTT
     1741 CGTCGGCATC TTTTTTGTCA TTGCGATTAA TCTGATCAAT CTGCTGAATC TGGGTGAGCT
     1801 GTTTTTGCGT AATATCCGCA ATAACGCTAG CATCATTGAG AGCTGGAAGC TGTGCCTGAA
     1861 ACGCAGCTTC TTCGCGAACC TGGAATTCCA TATTACGTGG TTAATCTCCG CGCTGGTGGT
     1921 GATTTACCTG AGCAACTATC AGGTCCAACA GCTGGCAAAT CTGATGGCCA TCTCCGCAAT
     1981 CACGAGCTAC TTTTTTAGCT ATGGTATTAG CATTGTACTG ATTTCCCTGT TTGTCTCGAG
     2041 CGAGTCAGGC GCGAACTGGA AGCTGTTCCT GTACCAGAAA GATGCAAAAG CGCTGACCAA
     2101 GACGAGCAGC AACTACAGCC TGCTGAGCAG CACCTCCGAC CTGAACACCG ACTTTTTTAT
     2161 TACCAAGAAC CAGCACGATT TCTTCCTGAA AAATAACTGG AAATTCCTGT TCGTCTGGCT
     2221 GATCTTGCTG GCCATTGGTG TGGTCATGCT CGTCCTGTAT TTGGTGCAAC CGAATCTGCT
     2281 GGGTGAATTC CTGGCAGCTG ACGTTGAGAG CAGCAACGGT ATCATTGCCG GCATTGGCAT
     2341 CGTGTCGCTG CTGTACTTGG CCTACTGCCT GATCCGCTAT GGCGTGGCAT ACTGTCTGAG
     2401 CTACCTGGTG TCATTCATTC TGCTGTGTAG CGGCCTGTTC GCGGTGATGT ATTTAACCAA
     2461 TTATCTGTTC AGCATCGACC AGAGCACTAT CCAACTGATT ACGTTCGTGT ACCTGTTCTG
     2521 GTTGTTTTTC GCCGCAAAGG TCAGCCAGAC CACCACCTGG ACCTACTTTT ACTGGTTCAA
     2581 GCGCTCGTTG AAAGATAAGG TTTTCATCAA AAATCTGTTC AACGATAACT TTAACAGCCA
     2641 GTGGAAAATC GACTTGATCG AGTCTAGCAG CCTGGTTTTC ATCTTCATTA TCTACAGCGG
     2701 TTTTAACTTT GGTGGCATTG ACGGCAATTT TAACCTGGTC ATCTTCTATC TGATCGCGAT
     2761 CGTCGGCCTG TTTGATGTGG CAACGGCGTT CTTGCCGATG TTCTGTTTCG GTCTGATTAA
     2821 CGGTTGGCTG TCTCCGTTCA ATTATGTCCA TTCTCGTCTG TCCCTGAAGC ATAAAAAACA
     2881 CCCATTTAAA GAGATCGACC AGATTGAAGA ACAGCTGATC GCCGGCATTA ATAGCTTTTG
     2941 AAGAGCTTAG AGACCGGCTG CCAAAGAAAC TGATTCGCTG AGGTGTCAAT CGTCGGAGCC
     3001 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     3061 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     3121 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     3181 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     3241 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     3301 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     3361 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     3421 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     3481 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     3541 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     3601 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     3661 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     3721 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     3781 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     3841 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     3901 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     3961 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     4021 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     4081 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     4141 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     4201 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     4261 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     4321 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     4381 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     4441 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     4501 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     4561 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     4621 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     4681 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     4741 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     4801 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     4861 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     4921 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG
//