LOCUS       Exported     4225 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4225
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2263..2310
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4158..4174
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4175..4206
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2191
                     /label="MG_RS01645"
                     /note="In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity)."
                     /translation="MATIQEIECDFLAKIAQKFTNAEIELINKAFYHAKTWHENQKRLSGEPFFIHPLRTALSLVEWNMDPITICAGLLHDIIEDTDQTEANIAMIFSKEIAELVTKVTKITNESKKQRHLKNKKENLNLKSFVNIAINSQQEINVMVLKLADRLDNIASIEFLPIEKQKVIAKETLELYAKIAGRIGMYPVKTKLADLSFKVLDLKNYDNTLSKINKQKVFYDNEWDNFKQQLKKILAQNQIEYQLESRIKGIYSTYKKLTVHEQNISKIHDLFAIRLITKSELDCYHILGLIHLNFLIDSKYFKDYIASPKQNLYQSIHTTVRLKGLNVEIQIRTQQMDNVSKFGLASHWIYKEQKEGLLAPALQLNYLVTKQKHSHDFLKRIFGTDIIKINVSASHEPNVIKQINVDSNNKLLDIAFENYPKQFAKLTKIEIDGVEINSFDTSVENEMLIEFYFGKNNNLKSKWIRYMNNPIYREKVKKSLAKLAKSGRYSELAFYEKELGEKQLKLASETEIQKRLNTLRIKKMSDYLALIECTNFTNDEHLLFLAKNNDKWNKLTKPLKFAFSKVVFHNSYFEQIEGIFITKIVIEPCCSKIPDMPEQVTGILTKNILSVHRYGCKNLQNKKQLKIIPLYWNIQQLKLKPRKFRSYININGVWSEKTINKICQTIINGDGYIEKIIPKINKQKDEFDLNITLFVNNYQQLLTLMDQITTKNISFSWKYL"
     primer_bind     complement(2315..2331)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2398..2986)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3177..4037)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4038..4142)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GGCGACCATC CAAGAGATTG AGTGCGATTT
       61 CCTGGCCAAA ATTGCGCAGA AATTTACGAA CGCAGAGATC GAACTGATTA ATAAAGCCTT
      121 CTATCACGCC AAAACCTGGC ATGAGAACCA AAAACGTCTG AGCGGTGAAC CGTTCTTTAT
      181 CCATCCGCTG CGCACCGCCC TGAGCTTAGT GGAGTGGAAT ATGGACCCGA TTACGATTTG
      241 CGCGGGTCTG TTGCACGACA TCATTGAGGA CACCGATCAG ACCGAGGCCA ATATCGCCAT
      301 GATCTTCAGC AAGGAAATTG CCGAGCTGGT CACCAAGGTT ACCAAAATTA CGAACGAGAG
      361 CAAGAAACAA CGCCACTTGA AAAATAAGAA GGAGAATCTG AACCTGAAAA GCTTCGTGAA
      421 CATTGCCATT AACAGCCAGC AGGAGATCAA TGTTATGGTC CTGAAGTTGG CGGATCGTCT
      481 GGATAATATT GCTAGCATCG AGTTCCTGCC GATCGAAAAA CAAAAAGTGA TCGCAAAAGA
      541 AACGCTGGAA CTGTATGCAA AGATCGCGGG TCGCATTGGC ATGTACCCGG TTAAAACTAA
      601 GCTGGCCGAT CTGAGCTTCA AAGTCCTGGA CCTGAAGAAT TATGACAATA CCCTGTCTAA
      661 GATTAATAAG CAAAAAGTGT TTTATGATAA CGAATGGGAT AATTTCAAAC AACAACTGAA
      721 GAAGATCCTG GCTCAGAATC AAATCGAGTA CCAACTGGAG AGCCGTATCA AAGGCATTTA
      781 TTCCACCTAC AAAAAGCTGA CCGTTCACGA GCAGAACATC TCTAAGATCC ACGATCTGTT
      841 CGCGATCCGC TTGATTACCA AGAGCGAGCT GGATTGCTAC CATATCCTGG GCTTGATCCA
      901 CCTGAACTTC TTGATTGACA GCAAGTATTT CAAGGACTAT ATCGCGAGCC CGAAACAGAA
      961 CCTGTACCAA AGCATTCATA CTACTGTCCG TCTGAAAGGT CTGAACGTTG AGATCCAAAT
     1021 TCGTACGCAG CAAATGGACA ATGTCAGCAA ATTCGGCCTG GCTAGCCATT GGATCTACAA
     1081 GGAACAAAAA GAGGGTTTGC TGGCACCGGC CCTGCAGCTG AATTATCTGG TGACCAAGCA
     1141 GAAGCACAGC CATGACTTCC TGAAACGCAT TTTCGGCACG GATATCATTA AGATTAATGT
     1201 CTCTGCGAGC CACGAACCGA ACGTGATCAA GCAGATCAAT GTGGACAGCA ATAACAAACT
     1261 GCTGGACATT GCCTTCGAAA ACTACCCGAA GCAATTTGCG AAGTTGACCA AAATTGAAAT
     1321 CGATGGCGTT GAGATCAACA GCTTCGATAC GAGCGTCGAA AATGAGATGT TAATCGAATT
     1381 TTACTTCGGC AAAAATAATA ATCTGAAGTC GAAGTGGATT CGTTACATGA ACAACCCGAT
     1441 CTATCGCGAG AAGGTTAAGA AAAGCCTGGC GAAACTGGCC AAGAGCGGTC GTTACTCGGA
     1501 ACTGGCGTTT TATGAAAAGG AACTGGGTGA GAAGCAACTG AAGCTGGCAA GCGAAACTGA
     1561 AATTCAAAAA CGCTTGAATA CCCTGCGTAT CAAGAAAATG AGCGACTACC TGGCGCTGAT
     1621 CGAATGTACT AACTTTACCA ATGACGAGCA TCTGCTGTTT TTGGCCAAAA ATAACGACAA
     1681 GTGGAACAAG CTGACCAAGC CGTTGAAGTT CGCATTTAGC AAGGTGGTGT TCCACAACTC
     1741 TTATTTTGAG CAGATCGAGG GCATTTTCAT CACGAAGATT GTCATTGAGC CGTGCTGCAG
     1801 CAAGATCCCG GACATGCCGG AACAGGTTAC GGGTATTCTG ACCAAGAACA TCCTTAGCGT
     1861 CCACCGCTAC GGTTGCAAAA ATCTGCAAAA CAAGAAACAA CTGAAAATCA TCCCGCTGTA
     1921 CTGGAATATC CAGCAACTGA AGCTGAAGCC GCGTAAATTT CGTAGCTACA TCAACATCAA
     1981 CGGTGTTTGG AGCGAAAAGA CCATTAACAA AATCTGCCAG ACGATCATCA ACGGTGATGG
     2041 CTACATCGAA AAAATTATTC CGAAAATTAA CAAGCAAAAA GACGAATTTG ACTTGAATAT
     2101 CACCCTGTTC GTCAATAATT ATCAGCAGCT GCTGACCCTG ATGGACCAGA TTACCACGAA
     2161 AAACATCTCT TTCTCGTGGA AATATCTGTG AAGAGCTTAG AGACCGGACC TTGACGCCTA
     2221 ATACTCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     2281 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     2341 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     2401 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     2461 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     2521 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     2581 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     2641 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     2701 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     2761 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     2821 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     2881 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     2941 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     3001 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     3061 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     3121 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     3181 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     3241 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     3301 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     3361 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     3421 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     3481 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     3541 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     3601 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     3661 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     3721 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     3781 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     3841 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     3901 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     3961 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     4021 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     4081 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     4141 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     4201 TTGACTTGGT CAGGTGGAGT GGGAG
//