LOCUS       Exported     3169 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3169
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1207..1254
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3102..3118
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3119..3150
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1135
                     /label="MG_RS01745"
                     /note="P37 is part of a high-affinity transport system."
                     /translation="MLFKKFTWVIPSLFLTIISTSLLISCATKSDNTLIFNISLDHNADTSIEKFFTVFSKKLSGKLNKKINVNFNIVDDSFTKINNIQANKADFAFVNSQAIASNNWFGYTPLIQTLTTAFKEDLELDYYEDGNLQKKAEKTNLLFLSPPYKEWDDIKQKWTGNRYDFLYEPSKLVSFYRSMILITGSASEITAIKKAWNEKNWNQFMKFGIGHGQTNSASRFELPDLLFRKHFAKNYPGLQNAINSDPDKFAVVRGREIGINKNIKIVFDDANSFSWTQNIKGSKRPFYTPIDPNDRLEILTYSDPLLYDIGIVSNNLSRIYQKAIGEIFIELAQSSEDLYGPSIGYNGYKMINDFEKEVVEIIEKTYGK"
     primer_bind     complement(1259..1275)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1342..1930)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2121..2981)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2982..3086)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GCTGTTCAAA AAGTTTACCT GGGTGATTCC
       61 TAGCCTGTTC CTGACCATTA TCTCCACCAG CCTGCTGATC AGCTGCGCGA CCAAGAGCGA
      121 TAATACCCTG ATTTTCAATA TTTCCCTGGA CCATAACGCA GATACCAGCA TCGAGAAGTT
      181 TTTTACTGTG TTCAGCAAGA AACTGTCCGG CAAGCTGAAT AAGAAGATCA ACGTTAATTT
      241 CAACATCGTC GATGACAGCT TTACCAAAAT TAACAATATT CAGGCAAACA AGGCCGATTT
      301 TGCGTTCGTT AACTCCCAAG CGATCGCATC TAACAACTGG TTCGGCTACA CCCCGCTGAT
      361 TCAGACCTTG ACCACCGCCT TTAAAGAGGA CCTGGAGCTG GACTACTACG AGGACGGTAA
      421 TCTGCAGAAG AAAGCGGAAA AGACCAACTT GTTGTTCCTC AGCCCGCCGT ACAAGGAGTG
      481 GGACGATATT AAGCAAAAGT GGACGGGTAA TCGCTATGAC TTCCTGTATG AACCGAGCAA
      541 ACTGGTCAGC TTTTACCGTA GCATGATCCT GATCACGGGT AGCGCTAGCG AGATTACGGC
      601 GATTAAGAAA GCATGGAACG AGAAAAACTG GAATCAGTTC ATGAAGTTCG GCATTGGTCA
      661 CGGTCAGACC AACAGCGCGA GCCGCTTCGA GCTGCCGGAT CTGCTGTTCC GTAAACATTT
      721 TGCGAAAAAT TACCCGGGTC TGCAAAATGC GATTAACTCT GATCCGGATA AATTTGCGGT
      781 GGTGCGTGGC CGTGAGATCG GCATTAATAA GAACATCAAA ATCGTGTTTG ACGATGCGAA
      841 TAGCTTCTCC TGGACTCAAA ACATCAAGGG CAGCAAGCGC CCATTCTATA CGCCGATTGA
      901 CCCGAACGAT CGCCTGGAAA TCCTGACGTA CAGCGACCCT TTACTGTACG ACATCGGTAT
      961 CGTCAGCAAC AACCTGAGCC GTATTTATCA GAAAGCAATT GGCGAGATTT TTATCGAGCT
     1021 TGCACAGTCA AGCGAGGACC TGTACGGTCC GAGCATCGGC TATAACGGTT ACAAGATGAT
     1081 CAACGATTTT GAGAAAGAGG TGGTCGAGAT TATCGAAAAG ACCTATGGTA AGTGAAGAGC
     1141 TTAGAGACCC CAGGAAAGTC GGCTAATCTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1201 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1261 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1321 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1381 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1441 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1501 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1561 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1621 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1681 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1741 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1801 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1861 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1921 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1981 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     2041 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     2101 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2161 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2221 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2281 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2341 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2401 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2461 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2521 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2581 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2641 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2701 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2761 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     2821 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2881 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2941 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     3001 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3061 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3121 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//