LOCUS Exported 3169 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3169 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1207..1254 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3102..3118 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3119..3150 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1135 /label="MG_RS01745" /note="P37 is part of a high-affinity transport system." /translation="MLFKKFTWVIPSLFLTIISTSLLISCATKSDNTLIFNISLDHNADTSIEKFFTVFSKKLSGKLNKKINVNFNIVDDSFTKINNIQANKADFAFVNSQAIASNNWFGYTPLIQTLTTAFKEDLELDYYEDGNLQKKAEKTNLLFLSPPYKEWDDIKQKWTGNRYDFLYEPSKLVSFYRSMILITGSASEITAIKKAWNEKNWNQFMKFGIGHGQTNSASRFELPDLLFRKHFAKNYPGLQNAINSDPDKFAVVRGREIGINKNIKIVFDDANSFSWTQNIKGSKRPFYTPIDPNDRLEILTYSDPLLYDIGIVSNNLSRIYQKAIGEIFIELAQSSEDLYGPSIGYNGYKMINDFEKEVVEIIEKTYGK" primer_bind complement(1259..1275) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1342..1930) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2121..2981) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2982..3086) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GCTGTTCAAA AAGTTTACCT GGGTGATTCC 61 TAGCCTGTTC CTGACCATTA TCTCCACCAG CCTGCTGATC AGCTGCGCGA CCAAGAGCGA 121 TAATACCCTG ATTTTCAATA TTTCCCTGGA CCATAACGCA GATACCAGCA TCGAGAAGTT 181 TTTTACTGTG TTCAGCAAGA AACTGTCCGG CAAGCTGAAT AAGAAGATCA ACGTTAATTT 241 CAACATCGTC GATGACAGCT TTACCAAAAT TAACAATATT CAGGCAAACA AGGCCGATTT 301 TGCGTTCGTT AACTCCCAAG CGATCGCATC TAACAACTGG TTCGGCTACA CCCCGCTGAT 361 TCAGACCTTG ACCACCGCCT TTAAAGAGGA CCTGGAGCTG GACTACTACG AGGACGGTAA 421 TCTGCAGAAG AAAGCGGAAA AGACCAACTT GTTGTTCCTC AGCCCGCCGT ACAAGGAGTG 481 GGACGATATT AAGCAAAAGT GGACGGGTAA TCGCTATGAC TTCCTGTATG AACCGAGCAA 541 ACTGGTCAGC TTTTACCGTA GCATGATCCT GATCACGGGT AGCGCTAGCG AGATTACGGC 601 GATTAAGAAA GCATGGAACG AGAAAAACTG GAATCAGTTC ATGAAGTTCG GCATTGGTCA 661 CGGTCAGACC AACAGCGCGA GCCGCTTCGA GCTGCCGGAT CTGCTGTTCC GTAAACATTT 721 TGCGAAAAAT TACCCGGGTC TGCAAAATGC GATTAACTCT GATCCGGATA AATTTGCGGT 781 GGTGCGTGGC CGTGAGATCG GCATTAATAA GAACATCAAA ATCGTGTTTG ACGATGCGAA 841 TAGCTTCTCC TGGACTCAAA ACATCAAGGG CAGCAAGCGC CCATTCTATA CGCCGATTGA 901 CCCGAACGAT CGCCTGGAAA TCCTGACGTA CAGCGACCCT TTACTGTACG ACATCGGTAT 961 CGTCAGCAAC AACCTGAGCC GTATTTATCA GAAAGCAATT GGCGAGATTT TTATCGAGCT 1021 TGCACAGTCA AGCGAGGACC TGTACGGTCC GAGCATCGGC TATAACGGTT ACAAGATGAT 1081 CAACGATTTT GAGAAAGAGG TGGTCGAGAT TATCGAAAAG ACCTATGGTA AGTGAAGAGC 1141 TTAGAGACCC CAGGAAAGTC GGCTAATCTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG 1201 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT 1261 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG 1321 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 1381 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 1441 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 1501 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 1561 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 1621 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 1681 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 1741 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 1801 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 1861 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 1921 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT 1981 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 2041 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 2101 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 2161 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 2221 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT 2281 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 2341 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 2401 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 2461 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 2521 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 2581 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 2641 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 2701 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 2761 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 2821 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 2881 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 2941 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT 3001 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 3061 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG 3121 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG //