LOCUS Exported 3481 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3481 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1519..1566 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3414..3430 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3431..3462 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1447 /translation="MTTKKGFKKRLKDFSKQQILALIILGAIDVFVIAAPYYVKNVVPNLHLYLGITEDEVATVTSIIGYVTLATQLPGGFLTNRFSSRKLLFLSAITTGAITFWLAANILTKNQQSHDALFIQYCVIWGLWGITSTLIFWTPLWKLASQQATKENQALGFGIQGAANGIWGLIFIFLIALIITSIFYPSGGSENADSKPFAAYAFIIAIMLVITGFTVLFFVKEKPIEKQSQTTLVSFKRNLNQILVTLKNWKLWLLSFFLMGMYVFQSTFAYYLLQMLQNAFLAPVVLVTVIGGIRTYALRSAVSVYLCRLADKCKSYILFLMICTVLGIVFVLAFILLGFVQTNSANITLITFSSILYIFTGILSWGMVTVRYNQIGEIDIGKNNYASSVGLLSFIGFSTDGWLYTVTAEVGKKYTVAGQSNTSNTGYQIIAAICLSIALFGLICGSIVFISNSMEIKRLNKASYRWRDLDNA" /label="MG_RS01775" /note="Derived by automated computational analysis usinggene prediction method: Protein Homology." primer_bind complement(1571..1587) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1654..2242) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2433..3293) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3294..3398) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AATGTCCTTA GGCAGTCGTC GGTCTCAAAT GACTACTAAG AAAGGCTTCA AGAAAAGACT 61 GAAGGATTTT AGCAAGCAGC AGATTCTGGC GCTGATTATC CTGGGTGCAA TCGACGTTTT 121 CGTTATCGCG GCGCCGTATT ACGTCAAAAA TGTCGTCCCG AATCTGCACC TGTATCTGGG 181 CATTACCGAG GACGAAGTGG CTACCGTGAC GAGCATCATC GGTTATGTCA CGCTGGCAAC 241 GCAGCTGCCG GGCGGCTTTT TAACGAACCG TTTTAGCAGC CGTAAACTGC TGTTCCTGAG 301 CGCAATCACC ACTGGTGCGA TCACTTTCTG GCTGGCGGCA AATATCCTGA CGAAGAATCA 361 GCAGAGCCAT GATGCCTTGT TTATCCAATA TTGCGTGATT TGGGGTCTGT GGGGTATCAC 421 GAGCACCCTG ATCTTTTGGA CGCCGCTGTG GAAGCTGGCG TCACAGCAAG CTACCAAGGA 481 AAATCAGGCG CTGGGCTTCG GTATTCAGGG TGCCGCAAAT GGCATTTGGG GTTTGATTTT 541 TATTTTTCTG ATCGCATTGA TTATTACCAG CATTTTTTAT CCGTCGGGTG GCTCAGAGAA 601 CGCCGATAGC AAGCCGTTCG CTGCTTACGC GTTCATTATC GCCATTATGC TGGTTATCAC 661 CGGTTTCACC GTGCTGTTCT TCGTTAAGGA GAAACCGATT GAGAAACAAT CGCAGACGAC 721 TCTGGTGAGC TTTAAGCGTA ACCTGAACCA GATTCTGGTG ACCCTGAAGA ACTGGAAATT 781 GTGGCTGCTG TCGTTTTTCC TGATGGGCAT GTATGTGTTC CAAAGCACCT TCGCCTATTA 841 TCTGCTGCAA ATGCTCCAAA ATGCCTTTCT GGCCCCGGTT GTTCTGGTGA CCGTCATTGG 901 TGGTATCCGC ACCTACGCCC TCCGTTCCGC GGTGAGCGTT TATCTGTGCA GACTGGCGGA 961 CAAATGTAAA TCTTACATTC TGTTTCTGAT GATCTGCACG GTTCTGGGTA TCGTGTTTGT 1021 TCTGGCGTTT ATCCTGCTGG GTTTCGTGCA AACCAACAGC GCGAATATCA CCCTGATCAC 1081 CTTTAGCAGC ATCCTGTACA TCTTTACCGG TATTCTGAGC TGGGGTATGG TGACGGTTCG 1141 CTACAATCAG ATTGGCGAAA TTGATATCGG TAAAAACAAC TACGCGTCCA GCGTTGGTCT 1201 GTTGAGCTTC ATCGGCTTTA GCACCGACGG TTGGCTGTAC ACCGTCACTG CAGAAGTAGG 1261 TAAGAAGTAC ACGGTTGCGG GCCAGAGCAA TACGTCGAAT ACCGGTTACC AGATCATTGC 1321 TGCTATTTGC CTGTCTATCG CGCTGTTTGG TCTGATTTGT GGTAGCATTG TTTTTATCAG 1381 CAATAGCATG GAGATTAAAC GCCTGAATAA AGCGAGCTAT CGTTGGCGTG ATCTGGATAA 1441 TGCCTGAAGA GCTTAGAGAC CCACTCGATA GGTACAACCG GCGCTGAGGT GTCAATCGTC 1501 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT 1561 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA 1621 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA 1681 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 1741 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1801 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 1861 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 1921 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1981 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 2041 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 2101 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 2161 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 2221 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT 2281 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 2341 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 2401 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 2461 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 2521 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA 2581 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 2641 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 2701 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 2761 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 2821 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 2881 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 2941 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 3001 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 3061 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 3121 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 3181 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 3241 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 3301 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 3361 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC 3421 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA 3481 G //