LOCUS       Exported     3481 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3481
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1519..1566
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3414..3430
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3431..3462
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1447
                     /translation="MTTKKGFKKRLKDFSKQQILALIILGAIDVFVIAAPYYVKNVVPNLHLYLGITEDEVATVTSIIGYVTLATQLPGGFLTNRFSSRKLLFLSAITTGAITFWLAANILTKNQQSHDALFIQYCVIWGLWGITSTLIFWTPLWKLASQQATKENQALGFGIQGAANGIWGLIFIFLIALIITSIFYPSGGSENADSKPFAAYAFIIAIMLVITGFTVLFFVKEKPIEKQSQTTLVSFKRNLNQILVTLKNWKLWLLSFFLMGMYVFQSTFAYYLLQMLQNAFLAPVVLVTVIGGIRTYALRSAVSVYLCRLADKCKSYILFLMICTVLGIVFVLAFILLGFVQTNSANITLITFSSILYIFTGILSWGMVTVRYNQIGEIDIGKNNYASSVGLLSFIGFSTDGWLYTVTAEVGKKYTVAGQSNTSNTGYQIIAAICLSIALFGLICGSIVFISNSMEIKRLNKASYRWRDLDNA"
                     /label="MG_RS01775"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
     primer_bind     complement(1571..1587)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1654..2242)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2433..3293)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3294..3398)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGTCCTTA GGCAGTCGTC GGTCTCAAAT GACTACTAAG AAAGGCTTCA AGAAAAGACT
       61 GAAGGATTTT AGCAAGCAGC AGATTCTGGC GCTGATTATC CTGGGTGCAA TCGACGTTTT
      121 CGTTATCGCG GCGCCGTATT ACGTCAAAAA TGTCGTCCCG AATCTGCACC TGTATCTGGG
      181 CATTACCGAG GACGAAGTGG CTACCGTGAC GAGCATCATC GGTTATGTCA CGCTGGCAAC
      241 GCAGCTGCCG GGCGGCTTTT TAACGAACCG TTTTAGCAGC CGTAAACTGC TGTTCCTGAG
      301 CGCAATCACC ACTGGTGCGA TCACTTTCTG GCTGGCGGCA AATATCCTGA CGAAGAATCA
      361 GCAGAGCCAT GATGCCTTGT TTATCCAATA TTGCGTGATT TGGGGTCTGT GGGGTATCAC
      421 GAGCACCCTG ATCTTTTGGA CGCCGCTGTG GAAGCTGGCG TCACAGCAAG CTACCAAGGA
      481 AAATCAGGCG CTGGGCTTCG GTATTCAGGG TGCCGCAAAT GGCATTTGGG GTTTGATTTT
      541 TATTTTTCTG ATCGCATTGA TTATTACCAG CATTTTTTAT CCGTCGGGTG GCTCAGAGAA
      601 CGCCGATAGC AAGCCGTTCG CTGCTTACGC GTTCATTATC GCCATTATGC TGGTTATCAC
      661 CGGTTTCACC GTGCTGTTCT TCGTTAAGGA GAAACCGATT GAGAAACAAT CGCAGACGAC
      721 TCTGGTGAGC TTTAAGCGTA ACCTGAACCA GATTCTGGTG ACCCTGAAGA ACTGGAAATT
      781 GTGGCTGCTG TCGTTTTTCC TGATGGGCAT GTATGTGTTC CAAAGCACCT TCGCCTATTA
      841 TCTGCTGCAA ATGCTCCAAA ATGCCTTTCT GGCCCCGGTT GTTCTGGTGA CCGTCATTGG
      901 TGGTATCCGC ACCTACGCCC TCCGTTCCGC GGTGAGCGTT TATCTGTGCA GACTGGCGGA
      961 CAAATGTAAA TCTTACATTC TGTTTCTGAT GATCTGCACG GTTCTGGGTA TCGTGTTTGT
     1021 TCTGGCGTTT ATCCTGCTGG GTTTCGTGCA AACCAACAGC GCGAATATCA CCCTGATCAC
     1081 CTTTAGCAGC ATCCTGTACA TCTTTACCGG TATTCTGAGC TGGGGTATGG TGACGGTTCG
     1141 CTACAATCAG ATTGGCGAAA TTGATATCGG TAAAAACAAC TACGCGTCCA GCGTTGGTCT
     1201 GTTGAGCTTC ATCGGCTTTA GCACCGACGG TTGGCTGTAC ACCGTCACTG CAGAAGTAGG
     1261 TAAGAAGTAC ACGGTTGCGG GCCAGAGCAA TACGTCGAAT ACCGGTTACC AGATCATTGC
     1321 TGCTATTTGC CTGTCTATCG CGCTGTTTGG TCTGATTTGT GGTAGCATTG TTTTTATCAG
     1381 CAATAGCATG GAGATTAAAC GCCTGAATAA AGCGAGCTAT CGTTGGCGTG ATCTGGATAA
     1441 TGCCTGAAGA GCTTAGAGAC CCACTCGATA GGTACAACCG GCGCTGAGGT GTCAATCGTC
     1501 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1561 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1621 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1681 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1741 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1801 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1861 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1921 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1981 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2041 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2101 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2161 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2221 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2281 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2341 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2401 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2461 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2521 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2581 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2641 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     2701 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2761 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2821 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2881 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2941 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     3001 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3061 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3121 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3181 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3241 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3301 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3361 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3421 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     3481 G
//