LOCUS       Exported     3298 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3298
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1336..1383
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3231..3247
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3248..3279
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1264
                     /label="MG_RS01805"
                     /note=""
                     /translation="MLNFKTLQAIDFQNKTVVLRSDFNVPMINGVISDSERILAGLDTIKFLVKKNCKIVLLSHLSRIKSLEDKLNNKKSLKPVAELLQQLLPTVKVQFSCKNTGAEVKQKVQALAFGEILLLENTRYCDVNDKGEIVKLESKNDPELAKFWASLGEIFVNDAFGTAHRKHASNAGIAKYVAKSCIGFLMEKELKNLSYLIQSPQKPFVVVLGGAKVSDKLKVVENLLKLADNILIGGGMVNTFLKAKGKATANSLVEKELIDVAKQILDKDTHNKIVLAIDQVMGSEFKDQTGITLDVSDKIQEQYQSYMSLDVGSKTIALFESYLKTAKTIFWNGPLGVFEFTNFAKGTSKIGEIIAKNKTAFSVIGGGDSAAAVKQMQLSDQFSFISTGGGASLALIGGEELVGISDIQKNS"
     primer_bind     complement(1388..1404)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1471..2059)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2250..3110)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3111..3215)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GCTGAACTTT AAAACCCTGC AAGCTATCGA
       61 CTTTCAGAAC AAAACGGTGG TTCTGCGTAG CGATTTCAAC GTGCCAATGA TCAATGGTGT
      121 TATCAGCGAT AGCGAGCGCA TCCTGGCGGG TTTGGATACT ATTAAATTTC TGGTTAAGAA
      181 GAACTGTAAG ATCGTCCTGC TGAGCCACCT GAGCCGTATT AAATCCCTGG AGGACAAGCT
      241 GAACAACAAA AAGAGCCTGA AGCCGGTCGC GGAACTGCTG CAACAACTGC TGCCGACGGT
      301 GAAGGTCCAA TTTAGCTGTA AAAACACCGG CGCCGAGGTG AAGCAAAAAG TTCAAGCCCT
      361 GGCGTTCGGT GAAATCTTGT TGCTGGAGAA CACGCGTTAC TGTGATGTAA ATGACAAAGG
      421 TGAGATCGTT AAACTGGAAT CCAAGAATGA TCCGGAACTT GCCAAATTTT GGGCGAGCCT
      481 GGGTGAAATT TTCGTTAATG ACGCTTTTGG TACCGCACAC CGTAAACATG CGTCCAACGC
      541 GGGTATCGCC AAATATGTCG CGAAATCTTG CATTGGTTTT CTGATGGAAA AGGAACTGAA
      601 GAATCTGTCC TACCTGATCC AATCCCCTCA GAAGCCGTTC GTCGTGGTTC TGGGCGGCGC
      661 GAAAGTCTCG GATAAACTGA AGGTCGTCGA AAATCTGCTG AAGCTGGCGG ATAACATCCT
      721 GATCGGTGGC GGCATGGTCA ACACCTTTCT GAAAGCAAAG GGCAAAGCAA CGGCGAACTC
      781 TCTGGTAGAA AAGGAATTGA TCGACGTGGC TAAGCAGATC CTGGACAAGG ACACCCACAA
      841 CAAGATCGTG TTGGCAATTG ATCAGGTTAT GGGCAGCGAA TTTAAGGATC AAACCGGTAT
      901 TACTCTGGAC GTGAGCGATA AAATCCAGGA GCAGTACCAG AGCTACATGT CCCTGGATGT
      961 GGGCAGCAAG ACGATCGCCC TGTTTGAGAG CTATCTGAAA ACCGCAAAGA CGATCTTTTG
     1021 GAACGGCCCG CTGGGTGTGT TCGAGTTCAC GAACTTTGCA AAAGGTACCA GCAAAATTGG
     1081 TGAGATTATT GCTAAGAATA AGACCGCGTT TAGCGTTATT GGTGGTGGTG ATAGCGCAGC
     1141 CGCCGTTAAG CAGATGCAAC TGTCGGACCA GTTCAGCTTC ATTAGCACCG GTGGCGGTGC
     1201 GAGCCTGGCG CTGATCGGCG GTGAGGAGCT GGTGGGCATT TCCGATATCC AAAAGAATTC
     1261 CTGAAGAGCT TAGAGACCCT GGAATCAGCC TTGTGTCTCG CTGAGGTGTC AATCGTCGGA
     1321 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1381 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1441 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1501 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1561 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1621 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1681 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1741 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1801 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1861 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1921 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1981 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2041 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     2101 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2161 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2221 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2281 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2341 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2401 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2461 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2521 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2581 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2641 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2701 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2761 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2821 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2881 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2941 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     3001 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     3061 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3121 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3181 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3241 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//