LOCUS       Exported     3862 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3862
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1900..1947
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3795..3811
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3812..3843
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1828
                     /label="MG_RS01890"
                     /note="Component of the cytoskeleton-like structure which stabilizes the shape of the wall-less mycoplasma. This cytoskeleton-like network of accessory proteins containing HMW proteins 1 to 5 allows the proper anchoring of cytadhesin proteins in the mycoplasmal membrane at the attachment organelle. Essential for successful surface parasitism (By similarity)."
                     /translation="MNDKQKAKINKAYNKLLKKINKRYPDVSVVYARDHKNKVHALYQDPESGNIFSLEKRKQLASNYPLFELTSDNPISFTNNIVSLNAYDDKNNLVTVQYDQDNNTFYDQNGNVLDVSSYTDEKKVPLINYLSSTQTSQEQPTQQDYPSIDAGLPKIEVDDQPKAAQHTTLETESEPDVFELNDSLNQPQQPTENLGDDQFVEKEVPPTQQLHQDLVHQQPVQVDSGSQNHSFNNSPSLKPPLVNKPAKLVQPEVKHIPQVEVQPKPQIVEPKIEPKPEVKHVSHVEIQPKPEVKPVVDSVPEVKQPEVKHVPHVEVQPKPVVDLKPQRIEPRIESKPEVIKHIPQVEVQPKAQMVEPRIEPKPETKYIPQVESTPQVEVHHWKPEVKTEYQPQQPLPTSGLQIKVVPRSAALLQSKLDTGFQPRQVERTTDSDITVSVSSHASLLEKINALNHQRIMSDIALKSDNTIKSSNFSRFYPENEYVATKYSDPLYSDTNQSLTSDRFSLDFDYTPKSRVNNYTPLRSTNFQNNAISNYRFSRTPSSYYPLTRRPWRLTNISSYRSSFHSPTRLSSFRRTSLPFSSSYDGLRRYPSRSYWSKDF"
     primer_bind     complement(1952..1968)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2035..2623)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2814..3674)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3675..3779)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATCAGTTTC TTTGGCAGCC GGTCTCAAAT GAACGACAAA CAAAAGGCGA AAATTAACAA
       61 GGCATATAAT AAGTTGCTGA AAAAGATCAA CAAACGTTAC CCTGACGTGT CTGTTGTTTA
      121 TGCGCGTGAC CATAAAAATA AGGTGCACGC ACTGTATCAG GATCCGGAAA GCGGTAATAT
      181 CTTTTCTCTC GAAAAACGTA AACAGCTGGC GAGCAATTAC CCGCTGTTCG AACTGACGAG
      241 CGACAACCCG ATCAGCTTTA CCAACAACAT TGTTAGCCTG AATGCATATG ATGATAAAAA
      301 TAATTTGGTT ACCGTTCAAT ACGACCAAGA TAATAACACC TTCTACGACC AAAACGGTAA
      361 CGTGCTTGAC GTTAGCTCCT ACACGGATGA AAAGAAAGTT CCGCTGATCA ACTACCTGAG
      421 CAGCACCCAG ACGTCCCAGG AGCAGCCAAC GCAGCAGGAC TACCCGAGCA TCGACGCGGG
      481 TCTGCCGAAA ATCGAGGTCG ACGACCAACC GAAGGCGGCC CAGCACACGA CCCTGGAAAC
      541 CGAGTCTGAA CCAGATGTGT TTGAGCTGAA TGACTCCTTG AATCAACCGC AACAACCAAC
      601 CGAGAATCTG GGCGACGACC AATTCGTTGA GAAGGAAGTC CCGCCAACCC AACAATTGCA
      661 CCAAGACTTA GTTCACCAGC AACCGGTCCA GGTGGACTCC GGCTCTCAGA ATCATTCCTT
      721 TAATAATAGC CCGAGCTTGA AACCGCCGCT GGTCAATAAA CCGGCAAAGC TGGTGCAGCC
      781 GGAAGTTAAG CACATCCCGC AAGTTGAGGT CCAGCCGAAA CCGCAGATTG TGGAGCCGAA
      841 AATCGAACCT AAACCGGAGG TTAAGCATGT GAGCCACGTC GAGATCCAAC CGAAACCGGA
      901 GGTTAAACCT GTTGTTGATT CTGTTCCGGA GGTGAAGCAG CCGGAAGTTA AACACGTTCC
      961 GCACGTGGAG GTTCAGCCGA AGCCGGTGGT TGACCTGAAG CCGCAACGTA TCGAGCCGCG
     1021 TATTGAGAGC AAACCGGAAG TTATTAAACA CATCCCACAG GTTGAAGTGC AACCGAAGGC
     1081 ACAGATGGTC GAACCGCGTA TTGAGCCGAA GCCGGAAACG AAGTACATCC CGCAAGTGGA
     1141 AAGCACCCCG CAAGTCGAGG TGCACCACTG GAAACCTGAG GTGAAAACCG AATACCAACC
     1201 TCAGCAACCT CTGCCGACCT CTGGTCTGCA GATCAAGGTT GTCCCGCGCA GCGCGGCTCT
     1261 GCTGCAAAGC AAATTAGATA CGGGTTTCCA ACCGCGCCAG GTGGAGCGTA CGACCGATTC
     1321 TGATATCACG GTCAGCGTTA GCAGCCACGC TAGCCTGCTC GAGAAAATCA ATGCGCTGAA
     1381 CCATCAGCGT ATCATGAGCG ATATCGCCCT GAAATCAGAT AACACTATCA AGAGCAGCAA
     1441 CTTTAGCCGT TTTTATCCGG AAAACGAGTA TGTTGCAACC AAGTATAGCG ATCCGCTGTA
     1501 TAGCGATACC AACCAGTCCC TGACGAGCGA CCGTTTCTCC TTAGATTTCG ACTATACGCC
     1561 AAAGAGCCGT GTCAATAACT ATACGCCGCT GCGTAGCACT AACTTTCAGA ATAATGCGAT
     1621 TAGCAACTAT CGTTTTAGCC GTACCCCGAG CTCGTACTAT CCTCTGACTC GTCGTCCGTG
     1681 GCGTTTGACC AATATTAGCA GCTACCGCAG CTCTTTTCAT TCGCCGACCC GTCTGAGCAG
     1741 CTTCCGCCGT ACCAGCCTGC CGTTTTCCAG CAGCTACGAT GGTTTACGCC GTTACCCGTC
     1801 AAGAAGCTAT TGGAGCAAGG ACTTTTGAAG AGCTTAGAGA CCGGCTCGAT ACAGTTTCAC
     1861 GTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1921 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1981 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     2041 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     2101 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     2161 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     2221 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     2281 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     2341 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     2401 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     2461 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     2521 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     2581 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     2641 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     2701 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     2761 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2821 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2881 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2941 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     3001 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     3061 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     3121 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     3181 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     3241 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     3301 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     3361 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     3421 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     3481 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     3541 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     3601 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     3661 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     3721 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3781 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     3841 ACTTGGTCAG GTGGAGTGGG AG
//