LOCUS       Exported     2827 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2827
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      865..912
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2760..2776
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2777..2808
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..793
                     /translation="MLSKKKNSGGGWINKISDKLSEINVAEYQVVSKHSNYLFYSRFGLLDTFVYFLFFLCFFLNNVLFLAGVFHTKQFTFNEVNGFNQFYLFWTVEKPTDIIQASITYQISQYGIANLVFGLISLALLVFLSFRWTVSLFFKSQTTKWERIGYTTGFFISIVVYLLWILLMVLFLVLLDQQFFERDTQKIQSATRFSLFFNVENNNGVYLSKLNSFGVFATAWAISLVFYSFFFIAIFAFNYNKTKLKQLFKKSKAK"
                     /label="MG_RS01975"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
     primer_bind     complement(917..933)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1000..1588)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1779..2639)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2640..2744)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GTTAAGCAAG AAGAAGAACA GCGGTGGTGG
       61 TTGGATCAAC AAGATCTCCG ACAAGCTGAG CGAAATTAAC GTCGCGGAGT ACCAGGTTGT
      121 CAGCAAACAC TCCAATTACC TGTTTTACTC GCGTTTCGGC CTGCTGGATA CGTTTGTCTA
      181 CTTCCTGTTT TTCCTGTGTT TTTTCCTGAA CAACGTTCTG TTCCTGGCGG GCGTTTTTCA
      241 CACCAAGCAG TTCACGTTTA ATGAAGTGAA CGGTTTCAAT CAGTTTTATC TGTTTTGGAC
      301 CGTAGAGAAG CCGACTGACA TCATCCAGGC TAGCATCACC TATCAGATCA GCCAGTATGG
      361 TATCGCGAAT TTGGTGTTCG GTCTGATCTC TCTGGCCCTG CTGGTCTTTC TGTCCTTCCG
      421 TTGGACTGTC TCGTTGTTCT TCAAGAGCCA AACGACCAAG TGGGAACGTA TTGGCTACAC
      481 CACCGGCTTT TTCATTTCGA TTGTGGTGTA TCTGCTGTGG ATTTTACTGA TGGTGCTGTT
      541 TCTGGTTCTG CTGGACCAGC AGTTTTTCGA GCGTGATACG CAGAAGATTC AGAGCGCGAC
      601 GCGTTTTAGC TTGTTCTTCA ATGTTGAGAA CAACAATGGT GTCTACCTGA GCAAACTGAA
      661 CAGCTTCGGT GTGTTCGCTA CGGCGTGGGC GATTAGCCTG GTGTTTTACT CTTTCTTCTT
      721 TATTGCGATT TTCGCTTTCA ACTACAACAA AACGAAATTG AAGCAACTGT TTAAGAAAAG
      781 CAAAGCAAAG TGAAGAGCTT AGAGACCGAG GATTCGGTCA GTTCACTCGC TGAGGTGTCA
      841 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
      901 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
      961 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1021 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1081 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1141 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1201 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1261 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1321 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1381 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1441 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1501 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1561 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     1621 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     1681 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     1741 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     1801 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     1861 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     1921 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     1981 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     2041 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     2101 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2161 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     2221 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     2281 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     2341 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     2401 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     2461 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     2521 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     2581 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     2641 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     2701 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     2761 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA
     2821 GTGGGAG
//