LOCUS       Exported     4333 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4333
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2371..2418
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4266..4282
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4283..4314
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2299
                     /label="MG_RS02000"
                     /note=""
                     /translation="MAVDKELEISDFDNELDEKTLLKELVQRTNNILFSPSKITAIPFERNLLEKTFFGTVDEAEKEKSIVSFFNWMIDLKVLDKKWDKNVLNHYANQLKTREEEQQTVDQTMAFQEVDDQSVLTKEIKTGFQELKPSVITAEDDKDEIKPEATKQVSFEELFNQPSEEINETKKPEVQIFSTDKVKEPEQFDDFYSIENLTKAINPVHKTIQYDQNDDQPFVVKRILKEQHPTKKVDELDDYNNKELLLENADLKKQIDDLKENNNDQIFDLEQEIDDLKRRLSEEKSKHLHTKKLQDDLLQENRDLYEQLQNKPVAINPLSDEVNEELENLKQEKALLSDQLDALKNKSSNVQQQLALLPVLNNQINELQNQLLTAREANQRLDLVEQENDFLKNELKKLHDNTSNDENEKYDDLLNQYELLFDENETKFDKLQVKQQALNLDYQKTISALKHENDVLLDEIEWTRSKDNDFNNTKNSFEEQKKALDEKLNGLTIQNQQLQDKIAELEEWNEEKSNLNTNQLVNLQQQLKDSQNLFNVAQDKLATLEEVNLALNEKINDLEDELSGSENSNNLLAKLQADHEILQESYGKLKTDFEKLKKNKLNDANEQYQDLLSAFEETNSELEKAKQSLSASDSENNQLKQQINSLENAKKELQFTPVTSDEHLDELETLKIEKEQLFLENQALQNQLQYFNDISANQTEEIKEASDEDKPVEIKKPRIKKRDFVIQNKDDKLAKLSKKERIQAYAERLAKINANE"
     primer_bind     complement(2423..2439)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2506..3094)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3285..4145)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4146..4250)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GGCTGTTGAC AAGGAACTGG AAATCTCTGA
       61 CTTCGATAAC GAACTGGACG AGAAAACGTT ACTGAAGGAA CTGGTGCAAC GTACGAACAA
      121 TATTCTGTTT AGCCCAAGCA AGATTACGGC CATTCCGTTC GAGCGTAATC TGTTGGAAAA
      181 AACCTTCTTT GGTACCGTGG ACGAGGCGGA GAAAGAAAAG AGCATTGTGA GCTTCTTCAA
      241 CTGGATGATC GACCTGAAGG TGCTGGACAA AAAGTGGGAC AAGAATGTGC TGAATCACTA
      301 CGCAAATCAG CTGAAAACCC GTGAAGAGGA ACAGCAAACG GTCGACCAAA CGATGGCTTT
      361 CCAGGAGGTC GACGATCAGA GCGTTTTGAC GAAAGAGATT AAGACCGGCT TTCAGGAACT
      421 GAAACCGAGC GTGATTACCG CCGAGGACGA CAAGGACGAA ATTAAGCCAG AGGCGACCAA
      481 GCAAGTTAGC TTTGAAGAAC TGTTCAACCA GCCTTCGGAA GAAATCAATG AAACCAAAAA
      541 GCCGGAAGTC CAAATCTTTA GCACTGACAA AGTCAAGGAA CCTGAGCAAT TCGACGACTT
      601 TTATAGCATC GAGAATCTGA CTAAAGCGAT TAATCCGGTC CATAAAACCA TCCAGTACGA
      661 TCAGAATGAT GATCAGCCGT TCGTTGTTAA ACGTATCCTG AAGGAACAGC ATCCGACGAA
      721 AAAAGTGGAT GAGCTGGATG ACTATAACAA CAAAGAACTG CTGCTGGAGA ATGCCGACCT
      781 GAAAAAACAG ATTGATGACT TAAAAGAGAA CAACAATGAC CAGATTTTCG ATCTGGAGCA
      841 AGAGATCGAT GATCTGAAGC GCCGTCTGAG CGAGGAAAAA AGCAAACACT TACATACCAA
      901 AAAACTGCAG GACGATCTGC TGCAAGAAAA CCGTGACCTG TACGAGCAGC TGCAAAATAA
      961 GCCGGTCGCA ATCAATCCGC TGTCCGATGA AGTTAACGAG GAGCTGGAAA ATCTGAAACA
     1021 GGAGAAGGCA CTGCTGAGCG ACCAGCTGGA TGCTCTGAAA AACAAAAGCA GCAACGTGCA
     1081 ACAACAACTG GCTCTGCTGC CGGTCCTGAA TAATCAAATC AACGAACTGC AGAATCAGTT
     1141 GCTGACCGCG CGCGAGGCGA ACCAACGTCT GGATCTGGTT GAACAGGAAA ATGACTTCTT
     1201 AAAAAATGAG CTGAAAAAAT TGCACGATAA CACGAGCAAC GACGAGAACG AAAAATACGA
     1261 TGATCTGCTG AATCAATACG AGCTGCTGTT CGACGAGAAC GAAACGAAAT TCGACAAATT
     1321 GCAAGTGAAG CAGCAAGCGC TGAATCTTGA TTACCAGAAA ACCATTAGCG CCCTGAAGCA
     1381 TGAGAACGAT GTTCTGCTGG ACGAGATCGA GTGGACCCGC AGCAAGGACA ATGACTTCAA
     1441 TAATACCAAG AATAGCTTCG AGGAGCAGAA AAAGGCATTG GATGAGAAAC TGAATGGTCT
     1501 GACTATTCAG AACCAGCAAC TGCAGGATAA AATCGCGGAG CTGGAGGAGT GGAATGAGGA
     1561 AAAGAGCAAT CTGAACACCA ACCAACTGGT TAATCTGCAA CAGCAGCTGA AGGACAGCCA
     1621 AAACTTATTT AACGTGGCAC AGGATAAGCT GGCTACCCTG GAGGAGGTTA ACCTGGCGCT
     1681 GAACGAGAAG ATTAATGACC TGGAGGATGA ACTGAGCGGT AGCGAGAATA GCAATAATCT
     1741 GCTGGCAAAG CTGCAAGCAG ATCATGAGAT CCTGCAGGAG AGCTACGGCA AACTCAAGAC
     1801 GGATTTCGAG AAGCTCAAGA AAAATAAACT GAATGACGCG AATGAGCAAT ATCAAGATCT
     1861 GCTGTCCGCA TTCGAAGAAA CCAATAGCGA ACTGGAGAAA GCGAAGCAAT CTCTGTCTGC
     1921 TAGCGACAGC GAGAACAACC AGCTGAAGCA GCAAATCAAC AGCCTGGAAA ATGCAAAGAA
     1981 AGAACTCCAG TTTACGCCGG TCACCTCTGA TGAGCATCTG GACGAACTGG AAACCCTGAA
     2041 GATTGAGAAA GAACAGCTGT TTTTAGAGAA CCAGGCGCTG CAAAACCAGC TGCAGTACTT
     2101 CAATGATATT AGCGCTAATC AGACGGAAGA AATCAAGGAA GCATCAGATG AAGATAAACC
     2161 GGTGGAGATC AAAAAGCCGC GCATTAAGAA ACGTGACTTC GTCATCCAAA ATAAGGATGA
     2221 TAAATTGGCG AAACTGTCCA AGAAGGAACG CATTCAAGCC TACGCAGAGC GTCTGGCAAA
     2281 GATCAACGCG AACGAGTGAA GAGCTTAGAG ACCCTTCATT GGTCTGGTGT CCTCGCTGAG
     2341 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     2401 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     2461 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     2521 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     2581 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     2641 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     2701 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     2761 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     2821 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     2881 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     2941 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     3001 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     3061 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     3121 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     3181 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     3241 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     3301 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     3361 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     3421 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     3481 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     3541 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     3601 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     3661 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     3721 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     3781 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     3841 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     3901 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     3961 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     4021 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     4081 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     4141 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     4201 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     4261 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     4321 GGTGGAGTGG GAG
//