LOCUS       Exported     3085 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3085
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1123..1170
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3018..3034
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3035..3066
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1051
                     /translation="MAQKEIINKKNTQKNSSFIESNNLTSFDFFDAKKNSEIETISTGSLNLDEALGSGGLPLGRIVELYGNESSGKTTIALNAVASFQKAGKTACYIDAEGALDLAYAKSIGIDLNKLLIAHPRHGENAFALIESLIKTNKISLIVIDSVAALIPKQELEGTIEEQTIGLHARMMSKGLRRIQSILPDSKTCVLFINQLREKPGVMFGNNEVTTGGKALRFYSSLRMEAKRVELLKDKFNNYVGIKTKVMVSKNKIAKPFGVAILEIMFNRGFVHEHEVIDLALKFNVVVRAGNSYSFNNESIAVGKEKLLNVLSEKPALFEQIKELTVQQLANKNSFQQTAS"
                     /label="MG_RS02065"
                     /note="Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage."
     primer_bind     complement(1175..1191)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1258..1846)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2037..2897)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2898..3002)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GGCGCAGAAA GAGATCATTA ATAAGAAAAA
       61 TACCCAAAAG AACTCCAGCT TCATTGAGAG CAATAATTTG ACTAGCTTCG ACTTCTTTGA
      121 TGCCAAGAAG AACAGCGAGA TTGAAACCAT CAGCACCGGC TCGTTGAATC TGGACGAGGC
      181 ACTGGGTTCC GGCGGTCTGC CACTGGGTAG AATCGTTGAG CTGTACGGTA ATGAGTCTAG
      241 CGGTAAAACC ACGATCGCTT TGAATGCCGT TGCCTCTTTC CAGAAGGCGG GTAAGACGGC
      301 CTGTTACATT GACGCTGAAG GCGCGCTGGA TCTGGCTTAC GCAAAAAGCA TCGGCATTGA
      361 TCTGAATAAG CTGTTAATTG CGCATCCGCG TCACGGCGAA AACGCTTTCG CGCTGATCGA
      421 AAGCCTGATT AAAACCAACA AAATCAGCCT GATCGTGATT GACAGCGTGG CGGCCTTGAT
      481 TCCGAAGCAG GAATTGGAAG GTACCATTGA GGAACAAACC ATTGGTCTGC ATGCGCGTAT
      541 GATGAGCAAA GGTCTGCGCC GTATTCAAAG CATCCTGCCG GACAGCAAGA CCTGTGTGCT
      601 GTTCATTAAT CAACTGCGCG AAAAACCGGG TGTGATGTTC GGTAACAACG AGGTCACCAC
      661 GGGTGGTAAG GCACTGCGTT TCTACAGCAG CCTGCGTATG GAGGCGAAAC GTGTGGAACT
      721 GCTGAAGGAC AAATTCAATA ACTATGTCGG TATCAAGACC AAAGTGATGG TCAGCAAGAA
      781 TAAGATCGCT AAGCCGTTTG GTGTGGCGAT TTTGGAAATT ATGTTCAACC GCGGTTTTGT
      841 GCACGAACAC GAAGTTATCG ATCTGGCCCT GAAGTTTAAT GTTGTGGTGC GTGCGGGCAA
      901 TAGCTACAGC TTTAACAACG AGAGCATTGC CGTCGGTAAA GAGAAATTGC TGAACGTGCT
      961 TTCCGAGAAA CCGGCGCTGT TTGAGCAAAT TAAAGAGCTG ACTGTGCAAC AGCTGGCGAA
     1021 CAAGAACTCC TTTCAGCAGA CCGCCTCCTG AAGAGCTTAG AGACCCCCCA CTGTTAGATT
     1081 GAGCTCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1141 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1201 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1261 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1321 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1381 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1441 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1501 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1561 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1621 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1681 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1741 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1801 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     1861 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     1921 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     1981 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     2041 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     2101 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     2161 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     2221 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     2281 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     2341 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2401 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     2461 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     2521 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     2581 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     2641 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     2701 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     2761 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     2821 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     2881 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     2941 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     3001 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     3061 TTGACTTGGT CAGGTGGAGT GGGAG
//