LOCUS       Exported     3400 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3400
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1438..1485
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3333..3349
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3350..3381
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1366
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
                     /translation="MKLTYNSSSGSNNQISFDSTSQGEKPSYIVEFTNSTNVGIKWTMVKKYQLDVPNVSSNMNQVLQELILEQPLTKYTLNSSLAKQKGKSQIEVHLGSNSNQWRSMRNSIGLNNNPSPNASTGFKLTTGNAYRKLDQSWPIYQPIDGTKQGKGKDSNGWSSTEENEAKNDAPLSTGGGTSDNASKFTKYLNTKQALERIGILFDGDGMRNVVTQLYYASTSKLAVTNNHIVVMGNSFLPSMWYWVVERSATTDSSSKPTWFANTNLDWGEDKQKQFVENQLGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQIIFSGFKAGSVGYDSSSSSSSSSSTKDQALAWSTTTSLDSKTGYKDLVTNDTGLNGPINGSFSIQDTFSFVVPYSGNHSNQISSGTIKTAYPVKKDEASQVAINSLINATPLNSYGSGIFFLFQPHPKVAKR"
                     /label="MG_RS02070"
     primer_bind     complement(1490..1506)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1573..2161)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2352..3212)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3213..3317)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GAAGTTGACC TACAACTCTA GCAGCGGTAG
       61 CAATAATCAG ATTAGCTTCG ACAGCACCAG CCAGGGTGAG AAGCCGTCCT ACATCGTCGA
      121 ATTTACTAAC TCCACGAACG TGGGCATTAA ATGGACGATG GTCAAAAAAT ATCAGCTGGA
      181 TGTCCCTAAT GTCAGCTCCA ACATGAACCA AGTGTTGCAG GAGCTGATCC TCGAGCAGCC
      241 GCTGACGAAG TACACCCTGA ATAGCAGCCT GGCGAAACAA AAAGGCAAGA GCCAAATTGA
      301 GGTCCATCTG GGTAGCAATA GCAATCAATG GCGCAGCATG CGTAATAGCA TCGGTTTGAA
      361 TAACAACCCG TCCCCGAATG CGAGCACGGG TTTCAAACTG ACCACCGGCA ACGCCTACCG
      421 CAAACTGGAC CAGAGCTGGC CGATCTACCA GCCGATTGAC GGTACCAAGC AGGGTAAGGG
      481 TAAGGATAGC AACGGTTGGA GCAGCACTGA AGAAAACGAG GCTAAAAATG ATGCACCGTT
      541 GAGCACGGGC GGTGGCACCT CTGACAACGC CAGCAAATTC ACGAAGTACC TGAACACCAA
      601 GCAGGCTCTG GAGCGTATTG GTATTCTGTT CGACGGTGAC GGCATGCGTA ACGTGGTTAC
      661 GCAACTTTAT TACGCGTCCA CCAGCAAGCT GGCAGTGACG AACAACCACA TCGTCGTTAT
      721 GGGTAACAGC TTCCTGCCGT CCATGTGGTA TTGGGTTGTT GAACGTAGCG CGACGACCGA
      781 TTCTAGCAGC AAGCCGACGT GGTTTGCTAA CACCAACTTG GATTGGGGCG AGGACAAACA
      841 AAAACAGTTT GTTGAGAATC AACTGGGCTA TAAAGAAACG ACCAGCACGA ATAGCCACAA
      901 CTTTCATTCC AAATCCTTTA CCCAGCCGGC GTACCTGATC TCCGGTATCG ACTCTGTGAA
      961 TGATCAGATC ATTTTTAGCG GTTTCAAGGC TGGCAGCGTC GGCTACGATA GCAGCAGCAG
     1021 CAGCAGCTCC TCTTCCAGCA CGAAGGATCA GGCTCTGGCG TGGTCTACTA CGACCTCCCT
     1081 GGATAGCAAA ACGGGCTACA AGGATCTGGT GACCAACGAT ACGGGTCTGA ACGGCCCGAT
     1141 CAACGGCAGC TTCAGCATCC AAGACACCTT TTCTTTTGTT GTGCCGTACT CTGGTAACCA
     1201 CAGCAATCAA ATTAGCAGCG GTACCATCAA GACCGCTTAC CCTGTGAAAA AAGACGAAGC
     1261 CTCTCAAGTC GCAATTAATA GCCTGATCAA CGCAACGCCG CTGAATAGCT ACGGTAGCGG
     1321 TATCTTCTTC CTGTTTCAGC CGCACCCGAA GGTGGCAAAA CGCTGAAGAG CTTAGAGACC
     1381 GGGTTTAGTC CGGCAATACT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1441 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1501 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1561 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1621 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1681 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1741 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1801 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1861 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1921 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1981 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     2041 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2101 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2161 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2221 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2281 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2341 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2401 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2461 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2521 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2581 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2641 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2701 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2761 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2821 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2881 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2941 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     3001 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     3061 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     3121 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     3181 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3241 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3301 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3361 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//