LOCUS       Exported     3031 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3031
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1069..1116
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2964..2980
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2981..3012
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..997
                     /label="MG_RS02130"
                     /note=""
                     /translation="MKRLFWNLKHKKAWLVLLLGTGMILSSCSNIDQSFFRELSVESVEKNTAYNQLPVNSTTFRNLVFGTRSYNDGNYVVVIATETDSSQINFLNGSTNQGTSSLSWGGTLGTTIRQVQNRYSTYPNGVKFLIWNDIAPGSVKWNPYARFPVVDRKNELASQEDKDNSNKLRRSDASAVRYREIVDFIQRTYGGNVANLINQSNVHAQTVGNDVTKAIVIAFRKDNLNKIRANFYGLDNSTNPNAPGSGQGDSTPPASSGEGGGSDGSSGGDSSSGNGQNTTPTSPQSSQPAVQRSQKSYGIKQHAVRVSVDFLNFLDSVYTPLN"
     primer_bind     complement(1121..1137)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1204..1792)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1983..2843)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2844..2948)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GAAACGTCTG TTTTGGAATC TGAAACACAA
       61 GAAAGCGTGG CTGGTGCTGC TGTTGGGTAC GGGTATGATC CTGAGCTCTT GTAGCAATAT
      121 TGATCAGAGC TTTTTTCGTG AGCTGAGCGT CGAATCTGTC GAGAAGAATA CGGCGTACAA
      181 CCAGCTGCCA GTTAACTCCA CCACGTTTCG TAACCTGGTC TTTGGTACCC GCAGCTACAA
      241 TGACGGTAAC TACGTGGTTG TGATCGCTAC CGAGACTGAC TCGAGCCAGA TCAATTTCCT
      301 GAACGGTTCT ACGAATCAGG GCACCAGCAG CTTAAGCTGG GGTGGTACCC TGGGTACGAC
      361 CATTCGTCAG GTTCAGAACC GTTACTCCAC GTACCCGAAT GGCGTGAAGT TCCTGATCTG
      421 GAACGATATC GCGCCTGGCT CCGTTAAATG GAATCCGTAT GCACGCTTTC CAGTTGTTGA
      481 CCGTAAGAAT GAGCTGGCGT CGCAGGAAGA TAAAGACAAT AGCAACAAGC TGCGCCGCAG
      541 CGACGCAAGC GCAGTTCGTT ATCGTGAGAT CGTGGACTTC ATCCAACGTA CCTATGGTGG
      601 CAATGTGGCG AACCTGATTA ACCAGAGCAA CGTTCACGCG CAAACCGTGG GTAACGATGT
      661 TACCAAGGCT ATTGTGATTG CGTTTCGTAA GGATAATCTG AACAAGATCC GCGCGAATTT
      721 CTACGGTTTG GACAACAGCA CCAATCCGAA CGCGCCGGGT AGCGGTCAGG GTGACAGCAC
      781 CCCGCCTGCT AGCAGCGGTG AAGGCGGCGG TAGCGACGGT AGCAGCGGTG GTGATAGCAG
      841 CTCTGGTAAC GGTCAGAATA CGACGCCAAC GAGCCCGCAG AGCAGCCAGC CGGCCGTCCA
      901 ACGTAGCCAA AAGAGCTACG GCATTAAGCA ACACGCCGTG CGTGTTAGCG TCGACTTCCT
      961 GAATTTCCTG GACAGCGTGT ACACGCCGCT TAACTGAAGA GCTTAGAGAC CGACGACTGC
     1021 CTAAGGACAT TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1081 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1141 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1201 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1621 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     1801 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     1861 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     1921 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     1981 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2041 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2101 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2161 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2221 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2281 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2341 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2401 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2461 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2521 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2581 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     2641 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     2701 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     2761 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     2821 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     2881 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     2941 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3001 AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA G
//