LOCUS       Exported     3235 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3235
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1273..1320
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3168..3184
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3185..3216
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1201
                     /label="MG_RS02140"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
                     /translation="MQPNYYRVIKKATSFSGLEMISHAYGQIAGVEVVGFYLWLITEANIQAFNSEIRTPISRLQNAFNPFEIKKNQVSEWIYKTISKLESLGLVRTFFSPKRSEITFCIIDPLDWKEFKQNKQLKEKLVEAMGKVEYDRNCLAFDQIDNLQFDNALEISANFEVNFTANQSDVWFSFNFEELHKELVKNKLLISLDEKAKTLINGYFEKYKLSLQQITDCIINSSTQENELDFQKLEMMFFQIVKNDTAPILETVSNNKDFFYKNEILDESTKKAITDCHVNFNSEKYLFLLYGKIDESQLQLVRQLRSDYQLLDKVINLVLDFSFWKNNGMWREKYILKIAQSIKINNSQNSYEKTLNNFIRALTLNKKHSLNNIKPVEKTISFTEYFEFIK"
     primer_bind     complement(1325..1341)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1408..1996)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2187..3047)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3048..3152)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAGAACGAA CAGGCACTAC GGTCTCAAAT GCAGCCGAAT TACTATCGCG TAATCAAGAA
       61 GGCAACTAGC TTCAGCGGCC TGGAAATGAT CAGCCACGCA TATGGCCAAA TTGCCGGCGT
      121 CGAAGTTGTG GGTTTCTACC TGTGGTTGAT CACCGAGGCA AATATTCAGG CTTTTAACAG
      181 CGAAATTAGA ACCCCGATCA GCCGTCTGCA AAATGCGTTC AACCCGTTTG AAATTAAAAA
      241 GAACCAGGTG TCTGAGTGGA TCTATAAAAC CATTAGCAAA TTAGAAAGCC TGGGCCTGGT
      301 TCGTACCTTT TTTAGCCCAA AACGTAGCGA GATTACTTTT TGTATCATTG ACCCACTGGA
      361 CTGGAAGGAG TTCAAGCAGA ACAAACAACT GAAAGAGAAG CTGGTGGAGG CCATGGGTAA
      421 AGTTGAGTAC GATCGTAACT GTCTGGCCTT TGACCAGATC GATAACCTCC AGTTTGACAA
      481 TGCCCTCGAA ATCAGCGCGA ACTTCGAAGT TAACTTTACG GCGAACCAGA GCGACGTGTG
      541 GTTTAGCTTC AATTTCGAAG AACTGCACAA AGAGCTGGTC AAGAATAAGT TGTTGATCTC
      601 TCTGGATGAA AAGGCTAAGA CCCTCATCAA CGGCTACTTC GAAAAGTACA AGCTGTCTCT
      661 GCAGCAAATT ACGGATTGTA TCATCAACTC CAGCACCCAA GAGAATGAAC TGGACTTCCA
      721 GAAATTAGAG ATGATGTTTT TCCAGATCGT CAAGAACGAC ACGGCACCGA TTCTGGAAAC
      781 TGTCTCCAAC AACAAGGACT TCTTTTATAA AAATGAGATC CTGGATGAGA GCACCAAGAA
      841 GGCAATCACG GACTGCCACG TGAACTTTAA TTCTGAAAAA TACCTGTTTC TGCTGTACGG
      901 TAAAATTGAT GAATCCCAGC TGCAGCTGGT GCGTCAACTG CGTTCCGACT ACCAGCTGCT
      961 GGATAAGGTC ATCAACCTGG TCTTGGATTT CAGCTTCTGG AAGAACAACG GTATGTGGCG
     1021 TGAAAAGTAT ATTTTAAAGA TCGCGCAGAG CATCAAAATC AACAACAGCC AGAATAGCTA
     1081 TGAAAAGACG CTGAACAACT TCATCCGCGC ACTGACGCTG AATAAAAAGC ATAGCCTGAA
     1141 TAATATTAAG CCGGTGGAAA AGACCATCAG CTTCACCGAA TACTTTGAAT TCATCAAGTG
     1201 AAGAGCTTAG AGACCGGCTC GATACAGTTT CACGTCGCTG AGGTGTCAAT CGTCGGAGCC
     1261 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1321 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1381 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1441 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1501 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1561 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1621 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1681 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1741 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1801 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1861 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1921 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1981 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     2041 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     2101 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     2161 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     2221 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     2281 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     2341 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2401 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2461 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2521 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2581 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2641 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2701 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2761 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2821 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2881 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2941 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     3001 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     3061 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     3121 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     3181 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG
//