LOCUS       Exported     4207 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4207
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2245..2292
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4140..4156
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4157..4188
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2173
                     /label="MG_RS02180"
                     /note="Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity)."
                     /translation="MNINFTPAGENRNFLQEIGRNINDEVLKNKVDPIIGRDNEIRRLIEILSRKSKNNPVLIGEPGVGKTAIVEGFVRRVVSNDVPLNLRDVEIYELSLSGLIAGTKFQGEFEKRINTILKQVKESNGRIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYIEKDGALERRFQKILINEPSSQEALTIMRGLKTRWELFHNITIFDSALVAAVEMSTRYINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIINLETEYAALKQDKENDNKQSKKEYLEKLKKQLDALKQKRDSLINEWKKEKADFENINKLKKEIEEFQTKLETYQSEGNYESASKILYSDIPRLKKELESAQQKYATSKHDLFKTEVSENEIAEVISQTTGIPLKKLLESEKDKLLHLGDEIKKRVKGQDEAIDAVVNTVIRGRVNINDPNKPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKALIRFDMSEYMEKHSVAKLIGAPPGYIGYEQSGLLTEAVRRKPYSVLLFDEIEKAHPDVTNVLLQVLDDGTLKDSQGRVVNFKNTLIIMTSNLGSNFLLEGKKDLAIQSLKKHFRPEFINRIDEIVFFNVLEKDTVLSIINSLLAQLSKRLNKQNLFFNFDSNLTEFIYKSSFDQQFGARPIKRFIDHSVATLIAKYILQGKIKKGVGYNIAVVKDNITITQNNKS"
     primer_bind     complement(2297..2313)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2380..2968)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3159..4019)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4020..4124)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATAGGAACC TCTTACGCGG GGTCTCAAAT GAACATCAAC TTCACCCCAG CGGGTGAGAA
       61 TCGCAACTTT CTTCAAGAGA TCGGTCGCAA TATTAACGAC GAAGTCCTGA AAAATAAAGT
      121 TGATCCGATC ATCGGCCGCG ACAATGAAAT TCGTCGTCTG ATCGAGATTC TGAGCCGTAA
      181 GAGCAAGAAT AACCCGGTGC TGATTGGCGA GCCGGGCGTC GGCAAGACCG CTATTGTCGA
      241 AGGTTTCGTT CGTCGTGTTG TGTCGAACGA CGTTCCGCTG AATCTGCGTG ATGTTGAAAT
      301 CTACGAATTG AGCCTGAGCG GCTTGATTGC CGGTACGAAG TTTCAAGGCG AGTTCGAGAA
      361 ACGTATTAAC ACTATTCTGA AACAAGTTAA GGAGAGCAAC GGTCGTATCA TTTTGTTCAT
      421 CGACGAAATT CACCAAATCG TCGGCCTGGG TCGTAACAGC AGCAGCGGCG CAATGGATAT
      481 CGCAAACATT CTGAAGCCGA TGTTGGCACG TGGCGAAATC AAAGTTATTG GCGCTACCAC
      541 CTTGAAGGAA TACCGTGAGT ACATCGAGAA GGACGGTGCG CTGGAACGTC GCTTTCAGAA
      601 AATTCTGATT AACGAACCGA GCTCGCAGGA GGCCCTGACG ATCATGCGCG GTCTGAAAAC
      661 GCGCTGGGAG TTGTTCCATA ACATCACCAT CTTTGACAGC GCCCTGGTTG CGGCCGTGGA
      721 AATGAGCACC CGCTACATTA ATGAGCGTAA TCTGCCGGAT AAGGCGATCG ACCTGATTGA
      781 CGAGGCGGCC GCTAAAATTA AAACGGAGAT GAGCAGCGAA CCGGTGGCAA TTGATAGCCT
      841 CAAGCGTGAG ATTATCAACC TGGAAACCGA ATATGCGGCT CTGAAGCAAG ACAAGGAGAA
      901 TGACAACAAG CAAAGCAAGA AAGAATATCT GGAAAAGTTG AAGAAGCAGT TGGACGCACT
      961 GAAACAGAAG CGTGATAGCC TGATTAACGA GTGGAAAAAA GAAAAAGCGG ACTTCGAAAA
     1021 CATCAATAAA CTGAAAAAGG AGATCGAAGA GTTCCAAACC AAGCTGGAAA CGTACCAGAG
     1081 CGAGGGCAAT TACGAAAGCG CAAGCAAGAT CCTGTACTCT GATATCCCGC GTCTTAAGAA
     1141 GGAACTGGAA AGCGCCCAGC AGAAATATGC GACCAGCAAG CACGATCTGT TTAAGACGGA
     1201 AGTCTCCGAA AACGAGATTG CAGAAGTCAT TAGCCAGACG ACCGGTATTC CGTTGAAAAA
     1261 ACTGCTGGAA AGCGAAAAGG ACAAGCTGCT GCACCTGGGT GATGAAATTA AGAAGCGTGT
     1321 CAAGGGTCAA GATGAGGCAA TCGATGCGGT CGTCAATACC GTTATTCGCG GTCGCGTTAA
     1381 CATTAACGAC CCGAACAAAC CGATCGGCAG CTTTATCTTC CTGGGCTCCA CGGGCGTTGG
     1441 TAAAACGGAG CTGGCAAAGA GCCTGGCGGA AGTCCTGTTC GACAACGAAA AGGCTCTGAT
     1501 CCGCTTCGAC ATGAGCGAGT ATATGGAGAA GCACTCGGTC GCTAAGCTGA TCGGTGCACC
     1561 GCCGGGTTAT ATTGGCTACG AGCAAAGCGG TCTGCTGACC GAGGCTGTTC GTCGTAAACC
     1621 GTACTCGGTG CTGCTGTTCG ATGAGATCGA GAAAGCCCAC CCGGACGTCA CCAATGTGCT
     1681 TCTGCAAGTG CTGGATGACG GTACCCTGAA AGACAGCCAA GGCCGTGTTG TTAACTTTAA
     1741 GAACACGCTG ATTATTATGA CCAGCAATTT GGGTAGCAAT TTCCTGCTGG AGGGCAAGAA
     1801 GGATCTGGCT ATTCAAAGCC TGAAGAAACA CTTCCGCCCG GAATTTATTA ATCGTATCGA
     1861 TGAAATCGTT TTCTTCAACG TTTTGGAAAA GGACACCGTT TTGAGCATCA TTAACTCGCT
     1921 GCTGGCGCAG CTGAGCAAAC GTCTGAATAA ACAGAATCTG TTTTTTAACT TCGACAGCAA
     1981 CCTGACGGAG TTCATCTACA AGTCAAGCTT CGACCAGCAG TTCGGCGCAC GTCCTATTAA
     2041 ACGTTTCATT GATCACAGCG TTGCAACGCT GATTGCGAAG TACATTCTTC AGGGTAAAAT
     2101 CAAGAAAGGT GTGGGCTATA ACATTGCTGT GGTTAAGGAT AACATCACGA TCACCCAGAA
     2161 CAATAAATCC TGAAGAGCTT AGAGACCCGC GGAAAATGGA AAACACTCGC TGAGGTGTCA
     2221 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     2281 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     2341 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     2401 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     2461 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     2521 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     2581 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     2641 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     2701 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     2761 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     2821 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     2881 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     2941 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     3001 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     3061 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     3121 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     3181 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     3241 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     3301 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     3361 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     3421 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     3481 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     3541 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     3601 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     3661 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     3721 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     3781 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     3841 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     3901 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     3961 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     4021 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     4081 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     4141 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA
     4201 GTGGGAG
//