LOCUS       Exported     3244 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3244
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1282..1329
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3177..3193
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3194..3225
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1210
                     /translation="MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPENWEKNNLVRRYGFHGTSYKYINEFLEKKFNKKPLNLIVCHLGNGASVCAIKQGKSLNTSMGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK"
                     /label="MG_RS02190"
                     /note="Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction."
     primer_bind     complement(1334..1350)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1417..2005)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2196..3056)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3057..3161)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GCAGAGCCAC AAAATTTTGG TGGTTAATGC
       61 GGGTAGCTCC TCGATTAAGT TCCAATTATT CAACGATAAA AAACAAGTGT TGGCTAAAGG
      121 CTTATGTGAA CGCATTTTCA TCGATGGCTT CTTCAAGCTG GAGTTCAACC AGAAGAAAAT
      181 CGAGGAGAAG GTCCAATTCA ATGATCACAA CCTGGCGGTT AAGCACTTCC TGAATGCGCT
      241 GAAGAAAAAT AAGATTATCA CGGAGTTGAG CGAGATTGGC CTGATTGGTC ACCGTGTTGT
      301 GCAAGGTGCC AATTACTTTA CGGACGCCGT GCTGGTTGAT ACGCACAGCT TGGCAAAGAT
      361 TAAAGAGTTT ATCAAGTTAG CGCCGTTGCA CAATAAGCCG GAGGCGGACG TTATCGAAAT
      421 TTTTCTGAAA GAAATTAAAA CCGCCAAAAA CGTCGCGGTT TTTGACACCA CCTTCCATAC
      481 CACGATCCCG CGTGAAAATT ATCTGTACGC GGTTCCAGAA AACTGGGAGA AAAACAATCT
      541 GGTTCGCCGC TATGGTTTCC ACGGTACCTC CTACAAGTAC ATCAATGAAT TTCTGGAGAA
      601 GAAGTTCAAC AAAAAGCCGC TGAATCTGAT CGTCTGTCAT CTGGGCAACG GTGCAAGCGT
      661 CTGCGCGATT AAGCAAGGTA AAAGCCTGAA TACCAGCATG GGTTTCACCC CGCTGGAAGG
      721 TCTGATCATG GGTACTCGTT CTGGTGACAT TGATCCGGCG ATTGTGAGCT ACATTGCGGA
      781 GCAACAGAAG CTGTCCTGCA ATGATGTCGT GAATGAACTG AACAAAAAGA GCGGCATGTT
      841 TGCGATCACC GGCAGCAGCG ACATGCGTGA CATTTTCGAC AAACCGGAGA TTAATGATAT
      901 CGCAATCAAG ATGTACGTGA ACCGTGTTGC CGACTACATT GCGAAGTATC TGAATCAGCT
      961 GTCTGGTGAG ATTGACAGCT TGGTCTTTAC GGGCGGTGTC GGTGAGAACG CCAGCTACTG
     1021 CGTTCAGCTG ATTATTGAAA AGGTGGCGAG CTTGGGCTTT AAAACTAACT CCAATCTGTT
     1081 CGGCAATTAC CAGGACTCTT CCCTGATCTC CACCAATGAG TCCAAGTACC AGATTTTTCG
     1141 CGTCCGTACC AACGAAGAAT TGATGATTGT GGAGGATGCG CTGCGCGTTA GCACCAACAT
     1201 TAAAAAGTGA AGAGCTTAGA GACCCGCATG TAGGCACCAT CTATCGCTGA GGTGTCAATC
     1261 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1321 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1381 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1441 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1501 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1561 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1621 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1681 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1741 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1801 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1861 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1921 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1981 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     2041 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2101 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2161 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2221 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2281 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2341 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2401 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2461 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2521 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2581 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2641 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2701 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2761 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2821 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2881 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2941 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     3001 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3061 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3121 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3181 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG
     3241 GGAG
//