LOCUS       Exported     3298 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3298
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1336..1383
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3231..3247
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3248..3279
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1264
                     /label="MG_RS02215"
                     /note=""
                     /translation="MINTFTYFEPEYLIDKNLIFLYFDFDAFFASVEELENPELVNQPLIVGNRFSRSVVSTCNYVARSYGIRSGMSILKALELCPNAIFAHSNFRNYRKHSKRIFSVIESTFSLKIDVLSVDEGVACFQNISFKKAFLIAKKIKNFVFQNLRIKISIGISDHFLIAKIFSNQAKPFGIKSCSVKDIKKKLWPLPITEIPGIGEKHIDLVFKNNFYKINDLAVCEDASLLKKVFGNFWESLKAVSLGKWYTDNNNQVKSRSFAVSETLEDLNYSNNQLNKKLTQIFDQLFIRLQLSSQVCKGIVVQLKSNDFIVNSHSNKMKKYSNDYRKLLSITKRLFNRLLINTEKNVRLIGISFFDLKKIDTDEGQKKSLFYQFIPKSISKLSEESSLDKLIFDINESFGFEIIKRANKLKS"
     primer_bind     complement(1388..1404)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1471..2059)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2250..3110)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3111..3215)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GATTAATACC TTTACCTACT TCGAACCGGA
       61 GTACCTGATC GACAAAAACC TGATCTTTCT GTACTTTGAT TTCGATGCGT TCTTCGCGAG
      121 CGTGGAGGAG CTGGAGAATC CGGAGCTGGT GAACCAACCG CTGATTGTCG GTAACCGTTT
      181 CAGCCGTAGC GTGGTGTCTA CGTGTAACTA CGTGGCTCGT TCCTATGGTA TTCGCTCCGG
      241 TATGTCTATC CTGAAGGCAC TGGAACTGTG TCCGAATGCA ATCTTTGCGC ACAGCAACTT
      301 TCGCAATTAT CGTAAACATA GCAAGCGTAT TTTTTCCGTT ATCGAATCGA CGTTCTCACT
      361 GAAAATCGAC GTGCTGAGCG TCGATGAGGG TGTTGCGTGC TTCCAGAACA TCTCGTTTAA
      421 AAAAGCGTTC CTGATTGCGA AGAAGATCAA AAACTTTGTG TTTCAAAATT TGAGAATTAA
      481 AATCAGCATC GGCATCAGCG ACCATTTCCT TATCGCGAAG ATCTTCAGCA ACCAGGCAAA
      541 GCCGTTTGGT ATTAAAAGCT GCAGCGTTAA AGATATTAAG AAAAAACTGT GGCCGCTCCC
      601 GATCACCGAA ATTCCGGGTA TTGGTGAAAA GCACATCGAT CTGGTGTTCA AAAATAACTT
      661 TTACAAAATC AACGATCTGG CGGTGTGCGA GGATGCAAGC CTGCTCAAGA AAGTTTTCGG
      721 TAACTTTTGG GAGTCGTTGA AAGCAGTCTC GCTGGGCAAA TGGTATACCG ATAACAACAA
      781 CCAAGTTAAG AGCCGCAGCT TTGCCGTGAG CGAAACCCTC GAGGACCTGA ATTACTCTAA
      841 TAATCAACTG AACAAAAAAC TGACCCAGAT TTTTGACCAA CTGTTTATTC GTCTGCAATT
      901 GTCCAGCCAA GTCTGTAAAG GCATCGTTGT GCAACTCAAG AGCAACGATT TTATTGTGAA
      961 CAGCCACAGC AATAAAATGA AGAAGTACTC CAACGATTAC CGTAAGCTGC TGAGCATCAC
     1021 CAAGCGTCTG TTCAATCGCC TGCTGATCAA CACCGAGAAA AACGTTCGTC TGATCGGCAT
     1081 CAGCTTTTTC GACCTGAAGA AAATCGATAC CGACGAGGGT CAGAAGAAAA GCCTGTTCTA
     1141 CCAGTTTATT CCAAAGAGCA TTTCCAAGCT GAGCGAGGAA AGCTCCTTGG ATAAGCTGAT
     1201 TTTCGATATT AATGAGAGCT TCGGCTTCGA GATCATCAAG CGTGCGAACA AACTGAAATC
     1261 CTGAAGAGCT TAGAGACCGG CATCGGACCC TTATAACTCG CTGAGGTGTC AATCGTCGGA
     1321 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1381 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1441 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1501 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1561 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1621 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1681 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1741 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1801 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1861 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1921 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1981 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2041 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     2101 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2161 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2221 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2281 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2341 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2401 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2461 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2521 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2581 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2641 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2701 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2761 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2821 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2881 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2941 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     3001 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     3061 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3121 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3181 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3241 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//