LOCUS       Exported     3736 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3736
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1774..1821
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3669..3685
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3686..3717
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1702
                     /label="MG_RS02260"
                     /note=""
                     /translation="MSSVNLSSFIDMLRLGCNNIAKNYEYINQLNVFPVPDGDTGTNMKVTITEAIKKLENEKSHIKSFSELGKNFTRDLLLFSRGNSGVIFSQIMKGFFSNIIVNKTSNNSELSIEDVANAFIVAQEVAYKNVSKPVEGTMLTVARLISNEFKSQKNRPKTLEKLFEQAVKVAWQAVKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSYYGGESNLDDNTLSTIEIKFNKFKEQHANEDEFGYCTEYVLRLGLKINQTVEKQKFHQKKFESKVNRIANSVVIASDKDNGFVKVHAHTLKPHLLLEMGLNYGEFEFVKIDNMNLQVNNKNNSPTKRVLKPAIVATVPTEAFAERIREDHDINAILCTDDTGAPSVFSLLEAVKLTNSSNVIFLLHDKNYFLSANETIKQLKHQKINADYVITANPVESIAALTVFNSDLSIHTNVKAMKRFIKEFASATITQASKSYKENKVMVNKNDFIAVTNKSIIASESQLTDCFFKTIDILSKKVKKPEFLLAYYGKDITEQDAKKMQALVEKKYKLFCEFSPGEQKVFSYIIGIQ"
     primer_bind     complement(1826..1842)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1909..2497)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2688..3548)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3549..3653)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATATCACGC AAAAGCACCG GGTCTCAAAT GTCCAGCGTT AATCTGAGCT CCTTCATTGA
       61 TATGCTGCGT TTGGGCTGTA ACAATATCGC CAAAAATTAT GAGTATATTA ATCAACTGAA
      121 TGTTTTCCCA GTTCCGGACG GTGACACGGG TACGAATATG AAGGTTACCA TCACGGAAGC
      181 GATTAAAAAA CTCGAGAACG AAAAGAGCCA CATTAAATCC TTCAGCGAAC TGGGTAAAAA
      241 TTTCACGCGC GATCTGCTGC TGTTCAGCCG TGGTAATAGC GGTGTCATTT TCAGCCAGAT
      301 TATGAAAGGC TTTTTCAGCA ACATCATCGT TAATAAAACC AGCAATAACT CCGAATTGAG
      361 CATTGAGGAT GTGGCAAATG CCTTTATTGT TGCTCAAGAG GTTGCGTATA AAAATGTCTC
      421 CAAACCGGTT GAGGGCACGA TGCTGACCGT CGCCCGTCTG ATTAGCAATG AGTTCAAATC
      481 ACAGAAAAAC CGTCCAAAGA CGCTGGAGAA ACTGTTTGAG CAGGCAGTCA AGGTGGCCTG
      541 GCAGGCAGTG AAGAAAACGC CGCAAATGCT GCCGGTTCTG AAAGCGTCTG GTGTTGTCGA
      601 TTCTGGCGCA TATGGCTTTG CATGCTTCCT GGAGGGCATG CTGAGCTACT ATGGCGGTGA
      661 AAGCAATCTG GATGATAATA CCCTGAGCAC CATTGAGATC AAATTTAATA AGTTCAAGGA
      721 ACAGCACGCT AATGAGGATG AATTCGGTTA TTGCACGGAA TACGTCCTGC GCCTGGGCCT
      781 GAAAATTAAT CAAACCGTTG AGAAGCAGAA GTTTCACCAG AAGAAATTCG AAAGCAAGGT
      841 TAATCGTATT GCCAACAGCG TTGTCATTGC CTCGGACAAA GACAATGGTT TTGTTAAGGT
      901 GCACGCGCAT ACCTTAAAAC CACATCTGCT GCTTGAAATG GGCCTGAACT ACGGCGAATT
      961 CGAGTTTGTC AAAATCGACA ATATGAATCT CCAGGTAAAT AACAAGAATA ACAGCCCGAC
     1021 GAAACGTGTC TTGAAACCGG CGATTGTAGC GACCGTGCCG ACCGAGGCAT TTGCGGAGCG
     1081 TATTCGTGAA GATCACGATA TCAACGCGAT CCTGTGCACT GATGACACCG GCGCGCCGTC
     1141 GGTCTTTAGC TTGTTGGAAG CTGTTAAGCT GACCAACAGC AGCAACGTTA TCTTCCTGCT
     1201 GCATGACAAG AACTACTTCC TGAGCGCCAA CGAAACTATT AAACAATTGA AACACCAGAA
     1261 AATTAACGCG GACTATGTGA TCACGGCCAA CCCGGTCGAA TCTATCGCAG CACTGACGGT
     1321 ATTTAACAGC GACTTGTCGA TCCACACGAA TGTGAAAGCT ATGAAGCGTT TCATTAAGGA
     1381 GTTCGCAAGC GCGACCATCA CCCAGGCAAG CAAGAGCTAT AAGGAGAATA AGGTGATGGT
     1441 TAATAAGAAT GATTTTATCG CGGTCACTAA CAAAAGCATT ATTGCTTCCG AGAGCCAGCT
     1501 GACCGATTGC TTTTTTAAAA CGATCGACAT CCTGAGCAAA AAGGTTAAAA AGCCGGAGTT
     1561 TCTGCTTGCG TACTACGGTA AGGACATTAC GGAGCAGGAT GCGAAGAAAA TGCAAGCGCT
     1621 GGTCGAGAAA AAGTATAAGC TGTTCTGCGA ATTTAGCCCG GGTGAACAGA AAGTTTTCAG
     1681 CTACATTATT GGCATCCAGT GAAGAGCTTA GAGACCCTAT TCGGTCTGCT CGTTGTCGCT
     1741 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1801 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1861 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1921 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1981 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     2041 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     2101 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     2161 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     2221 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     2281 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2341 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2401 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2461 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2521 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2581 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2641 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2701 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2761 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2821 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2881 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2941 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     3001 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     3061 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     3121 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     3181 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     3241 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3301 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3361 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3421 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3481 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3541 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3601 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3661 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3721 TCAGGTGGAG TGGGAG
//