LOCUS       Exported     3037 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3037
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1075..1122
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2970..2986
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2987..3018
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1003
                     /label="MG_RS02270"
                     /note=""
                     /translation="MISIDPQFIKNFSKKVKQFDKFSLFVHVNPDFDAFGSAFAFKTFLNTFFSEKKAYVMGSYNINADGRELFPFEQTDINDDFVKESLAIIFDTSNQERVLTQKHKLAKETVRIDHHPRTEKFADMEWIDSSFSATAEMIGYLILQMGYKLNDEIASYLYAGIITDTQRFWGPTTTPQTFALTAKLMETGFNRNKVHDAVYLKPLLEHKYFSYVLSKAKITKNGLAYALIKKGAYKHFGVVSPLPMVHALNNIKGVKIWTTVYFNESIKKWIGSIRSRNIPINNFAQMFNGGGHKYAAAFVLDEKNQFMKLVQIMDDFLAKQNENS"
     primer_bind     complement(1127..1143)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1210..1798)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1989..2849)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2850..2954)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GATTAGCATC GACCCGCAAT TCATTAAAAA
       61 TTTCAGCAAG AAGGTGAAAC AATTCGACAA ATTCTCCCTG TTTGTTCACG TGAACCCGGA
      121 CTTTGATGCT TTTGGTTCCG CTTTCGCGTT TAAAACTTTT CTGAACACCT TCTTCAGCGA
      181 GAAAAAGGCC TACGTTATGG GTAGCTATAA TATTAATGCG GACGGCCGCG AACTGTTCCC
      241 TTTCGAACAG ACGGATATCA ACGATGATTT CGTTAAAGAA AGCCTCGCGA TCATCTTTGA
      301 CACCAGCAAC CAAGAGCGTG TCCTGACGCA AAAGCACAAG CTGGCGAAGG AAACCGTTCG
      361 CATCGATCAT CATCCGCGTA CCGAAAAATT CGCCGATATG GAGTGGATTG ACTCTAGCTT
      421 CAGCGCGACT GCAGAGATGA TTGGTTATCT GATTCTGCAG ATGGGTTATA AGCTGAATGA
      481 CGAGATCGCA AGCTACCTGT ATGCGGGTAT CATCACCGAC ACCCAACGTT TCTGGGGTCC
      541 GACCACGACG CCGCAAACCT TTGCCCTGAC GGCGAAGCTG ATGGAAACCG GCTTCAACCG
      601 TAACAAAGTG CACGATGCAG TCTATCTCAA GCCGCTGCTG GAACACAAGT ACTTTAGCTA
      661 TGTTCTGAGC AAGGCTAAAA TCACCAAAAA TGGTCTGGCT TACGCGCTGA TTAAGAAGGG
      721 TGCCTATAAG CATTTTGGCG TGGTCAGCCC GCTGCCAATG GTGCACGCGC TGAACAATAT
      781 TAAAGGTGTG AAGATCTGGA CCACCGTGTA CTTCAACGAA TCTATCAAGA AGTGGATTGG
      841 CTCTATCCGC AGCCGCAACA TTCCGATCAA CAATTTCGCA CAGATGTTTA ACGGCGGTGG
      901 CCACAAATAC GCAGCGGCAT TCGTGCTGGA TGAGAAGAAT CAATTCATGA AACTGGTGCA
      961 AATTATGGAC GATTTTCTGG CGAAGCAGAA CGAGAATTCC TGAAGAGCTT AGAGACCGTG
     1021 TCACATTGTG TCGGACTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1081 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1141 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1201 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1261 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1321 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1381 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1441 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1501 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1561 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1621 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1681 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1741 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1801 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1861 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1921 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     1981 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2041 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2101 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2161 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2221 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2281 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2341 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2401 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2461 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2521 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2581 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2641 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2701 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2761 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2821 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2881 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     2941 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3001 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//