LOCUS       Exported     3901 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3901
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1939..1986
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3834..3850
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3851..3882
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1867
                     /label="MG_RS02310"
                     /note="NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34."
                     /translation="MSFIITVIGAGHAGLEAAFIVSKFNIKVNLLVLDINHLGSCPCNPSIGGPAKGIVTREIDVLGGMQAIAADNNALQYKLLNSSKGPAVQAIRAQIDKIGYKNWFQSQVKLNKNINLIQSEAINLIVRNEKIKGVILKDGSELLSDAVIITTGTYLRSKTYCGNTVKNQGPDQSKNSEKLSTNLINRGFKTIRLKTGTPPRILKTSLDYNQMELEINNNQNLAFSTTNKNFLPLEKQIPCYLVHTNQKIHDLILKNLKKSAMFNGSISAQGPLYCPSIEDKVFKFSQKPRHQIFVEPESLSLDTIYLAGLSTSFTPEIQKEIIQLLPGFQNAEIKKFGYAIEYDAFLSNQLKPTLETKLIENLYFAGQINGTSGYEEAAGQGLMAGINAALKLLKKPPFILQRNEAYIGVMINDLVTKTISDPYRLLTSRAEYRLWLRNDNVQERLIKKSFELGLTDKKTYELFLKKEKKKQELISFLKNTQVGKVKALKFTNKNTAQSLYDFNKRSEINLDKLIKDLPEKYQLDSETLKQIEIEIKYEGYIKKNEKYFKGLDKLSKIKIPHTFDYHKVKNLASEAIFKLSNFKPSNLAIASQIAGVNFNDIIAIKHFLKTYE"
     primer_bind     complement(1991..2007)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2074..2662)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2853..3713)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3714..3818)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAGAACGAA CAGGCACTAC GGTCTCAAAT GAGCTTCATC ATTACTGTAA TCGGCGCGGG
       61 TCACGCGGGT TTGGAGGCAG CATTTATCGT TTCGAAGTTC AATATCAAGG TCAATTTGCT
      121 GGTTCTGGAT ATTAATCACC TGGGCAGCTG TCCGTGTAAC CCGTCCATTG GCGGTCCTGC
      181 CAAAGGCATT GTTACCCGCG AGATCGATGT CCTGGGTGGC ATGCAGGCGA TCGCGGCGGA
      241 CAATAATGCG CTGCAATACA AGCTGCTGAA TAGCTCGAAA GGTCCGGCGG TTCAGGCAAT
      301 TCGTGCGCAG ATTGATAAAA TTGGCTACAA GAACTGGTTT CAATCACAGG TGAAACTGAA
      361 CAAGAACATT AACTTAATCC AGTCCGAGGC GATTAACCTG ATTGTGCGTA ATGAGAAAAT
      421 TAAAGGCGTG ATCTTGAAGG ACGGCAGCGA GCTGCTGTCA GATGCCGTCA TCATCACGAC
      481 GGGTACCTAT CTGCGTTCCA AAACCTACTG TGGCAACACC GTGAAAAATC AAGGCCCAGA
      541 TCAATCCAAA AATAGCGAAA AACTGAGCAC GAACTTGATC AACCGCGGTT TCAAGACCAT
      601 CCGCCTCAAG ACCGGTACCC CGCCACGCAT CCTGAAAACT AGCCTGGACT ACAACCAAAT
      661 GGAGCTGGAG ATCAACAATA ACCAAAACTT GGCGTTTTCG ACGACGAACA AGAATTTTCT
      721 GCCGCTGGAA AAGCAAATTC CGTGCTACCT GGTGCACACT AACCAGAAGA TCCACGATCT
      781 GATTCTGAAG AATCTGAAAA AGAGCGCCAT GTTCAATGGT TCCATCAGCG CGCAAGGTCC
      841 GCTGTACTGC CCGAGCATTG AGGATAAGGT GTTCAAGTTC AGCCAAAAAC CGCGTCATCA
      901 AATCTTCGTA GAGCCGGAAA GCTTGTCTCT GGATACCATT TACCTGGCAG GCCTGTCCAC
      961 CAGCTTTACC CCGGAAATTC AAAAAGAGAT TATTCAGCTG CTGCCGGGCT TCCAGAACGC
     1021 AGAAATCAAA AAGTTTGGTT ACGCTATCGA ATACGACGCA TTTCTGAGCA ACCAGCTGAA
     1081 GCCGACCCTG GAAACCAAAT TGATTGAAAA CTTGTATTTC GCGGGTCAAA TTAACGGCAC
     1141 GAGCGGTTAT GAAGAGGCTG CTGGCCAAGG CTTAATGGCT GGTATCAACG CCGCTCTGAA
     1201 ACTGCTGAAG AAACCGCCGT TTATCCTGCA GCGCAATGAA GCGTACATTG GTGTTATGAT
     1261 CAACGACCTG GTGACCAAGA CGATTTCCGA CCCTTATCGT CTGCTGACGA GCCGTGCGGA
     1321 ATATCGTTTG TGGCTGCGTA ATGACAACGT CCAAGAACGT TTGATTAAAA AGAGCTTCGA
     1381 GTTGGGCCTG ACCGACAAGA AAACCTACGA ACTGTTTCTG AAGAAAGAGA AAAAGAAGCA
     1441 GGAACTGATC AGCTTTCTGA AAAATACGCA AGTGGGCAAA GTGAAAGCTC TGAAATTCAC
     1501 CAACAAGAAC ACGGCACAAA GCCTGTACGA TTTCAACAAG CGTTCTGAGA TTAATCTCGA
     1561 CAAATTGATT AAGGATCTGC CGGAGAAGTA CCAGCTGGAT AGCGAAACCT TGAAGCAGAT
     1621 TGAGATTGAA ATCAAATATG AGGGTTACAT CAAGAAAAAC GAGAAATATT TTAAGGGTCT
     1681 GGATAAGCTG AGCAAGATCA AGATTCCACA TACCTTCGAC TACCATAAAG TTAAAAATCT
     1741 GGCGAGCGAA GCTATCTTTA AACTGAGCAA CTTTAAGCCT TCCAACCTGG CGATCGCGAG
     1801 CCAGATTGCC GGCGTCAATT TCAACGATAT TATCGCAATT AAACACTTCC TGAAAACCTA
     1861 CGAGTGAAGA GCTTAGAGAC CGCCACGGCG AAATTTAATG TCGCTGAGGT GTCAATCGTC
     1921 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1981 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     2041 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     2101 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     2161 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     2221 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     2281 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     2341 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     2401 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2461 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2521 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2581 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2641 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2701 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2761 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2821 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2881 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2941 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     3001 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     3061 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     3121 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     3181 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     3241 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     3301 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     3361 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     3421 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3481 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3541 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3601 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3661 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3721 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3781 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3841 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     3901 G
//