LOCUS       Exported     4570 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4570
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2608..2655
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4503..4519
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4520..4551
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2536
                     /label="MG_RS02525"
                     /note=""
                     /translation="MLKKISLFDNLEQWVDLSLLMIRTSFLDANYFGVAKLSLFKYGNYLIDNLNQAQWKTLSQVLQLNKQPYSGFLLVNNHQYLPLQKIKFNLCEEIDLRNKNKWLFPNSFSIFLKQDLSYKKDQNYFNLVNIPKQLKWFLGFNALNDLFRFSILKIENVNQKTSKVINQFFTSKFINSIYFEQVDFEYNNFLTLIEKNERELVDFNCMFFNQLFTFNNKVIKSFFERYYALNEQKNNLKNKLKIAAYEAKSRDNSYFEKLQIERAKQRLFDAKKTFSKQHKNALQLINSFTFSLIFSGWKIKWRSFFNFYRKRILEKKIVSKRIKTRILLLKEIKKMDLLSPKLLVETIDESINLVNKIFANIQNLYQEIISLKSGPNLNWKYQTINQELKIMSARFILTKKEVHEFLIKARLSFIKNFVKQSNNLYVHEKLVNELKENILLNQNHYRGKHNNSFSVLIQKTVFKQFIDTVQYAIGLISMRKSLETFNFAFQIKQYFYESLLANYQKINWQKYELNNLYFVLISLWNSLSKKFQQFFNKYEFLSCGSNDFIWNEGRSEPHFSLLKDKLKNDLNSPKWNFLVDKVINKYLDLNFETPVSFLLASRGFSSTTDTTNKETKNDLKQKLKKCKQKYKLLLKDINLVKWIFRDETNQKLQKIKFIMKRYCILNKTLHLRLKKTNKKIKRTFFIDSEECNINRLEASNKLYLNVLNSQLKTINFFLRSQKNLKKLNFINNINLLINQTKKNGISSWMLFSDLNKINKNESLKLYLLFKLLLNPKLLMINCCNDFTNHAYNFIRGLLISYQNKQGIALIFNDPNNKLVKNFSIKNIDFETGMKK"
     primer_bind     complement(2660..2676)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2743..3331)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(3522..4382)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4383..4487)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GTTGAAGAAA ATCAGCCTTT TTGACAATCT
       61 GGAACAATGG GTTGATCTGA GCCTGTTAAT GATTCGTACC AGCTTTCTGG ATGCGAATTA
      121 CTTCGGTGTG GCAAAACTGA GCCTGTTTAA GTACGGTAAC TACCTGATTG ATAACCTGAA
      181 TCAGGCGCAG TGGAAAACCC TGAGCCAGGT TTTGCAGCTG AACAAGCAGC CGTACTCCGG
      241 TTTCCTGCTG GTGAATAACC ATCAGTACTT GCCGCTGCAG AAGATCAAAT TCAACTTATG
      301 CGAAGAAATT GATCTGCGTA ACAAGAATAA ATGGCTGTTT CCAAACAGCT TCAGCATTTT
      361 CCTGAAGCAG GATCTGTCTT ACAAAAAAGA CCAAAACTAC TTTAATCTGG TGAATATCCC
      421 GAAGCAACTG AAATGGTTCT TAGGTTTCAA TGCGCTGAAT GACTTGTTCC GTTTTTCAAT
      481 TCTGAAAATC GAAAACGTCA ATCAAAAGAC CTCCAAGGTG ATCAATCAAT TCTTTACTAG
      541 CAAGTTCATT AATAGCATCT ATTTCGAACA GGTGGACTTC GAGTATAATA ATTTTCTGAC
      601 CCTGATCGAG AAGAATGAGC GCGAACTGGT GGACTTTAAC TGCATGTTTT TCAACCAGCT
      661 GTTCACCTTT AATAACAAAG TGATTAAAAG CTTTTTTGAG CGTTATTACG CGTTGAATGA
      721 ACAGAAAAAT AATCTGAAAA ACAAACTGAA GATTGCGGCC TACGAGGCGA AGTCTCGTGA
      781 CAACTCGTAC TTTGAGAAAC TGCAAATTGA ACGTGCGAAA CAGCGTCTGT TTGATGCTAA
      841 GAAAACCTTT TCTAAGCAGC ACAAAAATGC ATTGCAGTTG ATCAACAGCT TTACCTTCTC
      901 TCTGATCTTC AGCGGTTGGA AGATCAAATG GCGTAGCTTT TTTAATTTCT ATCGTAAGCG
      961 TATCTTGGAG AAGAAAATCG TTAGCAAACG TATCAAAACT CGTATCCTGC TGCTGAAGGA
     1021 GATCAAAAAG ATGGATCTGC TGAGCCCGAA GCTGCTGGTG GAAACCATCG ATGAAAGCAT
     1081 CAACCTGGTT AACAAAATTT TCGCGAATAT CCAGAATCTG TACCAGGAAA TTATCTCCTT
     1141 GAAAAGCGGC CCGAACCTGA ACTGGAAATA CCAGACCATC AACCAAGAGC TGAAAATTAT
     1201 GAGCGCGCGC TTTATTCTGA CGAAAAAAGA GGTCCATGAA TTCCTGATCA AGGCGCGCCT
     1261 GAGCTTTATT AAGAACTTCG TTAAGCAAAG CAACAATCTG TATGTGCACG AAAAACTGGT
     1321 CAACGAACTG AAGGAAAATA TCCTGCTGAA CCAAAACCAT TACCGTGGCA AGCATAACAA
     1381 CTCTTTTAGC GTCCTGATTC AAAAGACGGT CTTTAAACAA TTCATCGATA CGGTCCAGTA
     1441 CGCAATCGGT TTAATTAGCA TGCGCAAGAG CCTGGAAACG TTCAACTTCG CATTCCAAAT
     1501 CAAGCAATAT TTTTATGAGA GCTTGCTGGC CAATTACCAA AAAATTAATT GGCAGAAATA
     1561 CGAGCTGAAT AATCTGTACT TCGTCCTGAT CAGCCTTTGG AACAGCCTGT CTAAGAAGTT
     1621 TCAACAATTC TTTAACAAAT ATGAATTTCT GAGCTGCGGT AGCAACGACT TCATCTGGAA
     1681 CGAGGGTCGC TCCGAGCCGC ACTTCAGCCT GCTCAAGGAC AAACTGAAGA ATGACCTGAA
     1741 CAGCCCGAAA TGGAACTTCC TGGTTGATAA AGTCATTAAC AAATACCTGG ATTTAAACTT
     1801 CGAAACGCCG GTCAGCTTTC TGCTGGCATC TCGTGGCTTC AGCAGCACCA CCGACACCAC
     1861 GAATAAGGAA ACGAAAAACG ATCTGAAACA AAAGTTGAAG AAGTGCAAAC AGAAATATAA
     1921 GCTGCTGCTG AAGGATATCA ATCTGGTGAA ATGGATTTTT CGTGATGAAA CCAATCAGAA
     1981 GCTGCAAAAA ATTAAATTCA TTATGAAGCG TTACTGCATT CTGAATAAGA CGCTGCATCT
     2041 GCGCCTGAAG AAAACCAACA AGAAGATCAA GCGTACGTTC TTCATCGACT CTGAAGAATG
     2101 CAATATTAAT CGTCTGGAAG CCAGCAACAA ACTGTACCTG AACGTTCTGA ACAGCCAGTT
     2161 AAAGACGATC AACTTCTTCT TACGTAGCCA GAAGAACCTG AAAAAACTGA ACTTCATTAA
     2221 TAATATCAAC TTGCTGATTA ACCAGACCAA GAAAAATGGT ATCAGCAGCT GGATGCTGTT
     2281 CAGCGACCTC AATAAGATCA ATAAAAATGA AAGCCTGAAA TTATACCTCT TGTTCAAACT
     2341 GCTGCTGAAT CCGAAGCTGT TGATGATTAA TTGCTGTAAT GACTTCACTA ACCATGCCTA
     2401 CAATTTTATC CGTGGTCTGT TGATCTCCTA CCAAAATAAA CAAGGTATCG CCCTGATTTT
     2461 CAACGATCCG AACAATAAAC TGGTTAAGAA TTTCAGCATC AAGAACATTG ATTTCGAAAC
     2521 CGGTATGAAA AAGTGAAGAG CTTAGAGACC GCGTATACCT TGCTTCCTGT CGCTGAGGTG
     2581 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     2641 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     2701 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     2761 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     2821 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     2881 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     2941 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     3001 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     3061 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     3121 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     3181 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     3241 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     3301 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     3361 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     3421 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     3481 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     3541 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     3601 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     3661 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     3721 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     3781 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     3841 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     3901 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     3961 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     4021 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     4081 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     4141 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     4201 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     4261 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     4321 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     4381 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     4441 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     4501 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT
     4561 GGAGTGGGAG
//