LOCUS Exported 2578 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2578 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 616..663 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2511..2527 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2528..2559 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..544 /label="MG_RS02550" /note="" /translation="MKNNISDVKLGLLAAKIYWKSWRFLELTEDDIISIALHAEQDSKKRFNPEFGLSFDNYLKLNGANFIRSSFRSMVNKVELLDSKSKYSLEKQNTVLNTPENYLRSLEFKEIITKAFNKAKNDQERKVFSLYVKGYKNFEIAKKLNISPRRVRYLLDLFKSYIKLLTERYGY" primer_bind complement(668..684) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(751..1339) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1530..2390) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2391..2495) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 AAGGTATGTA TAGCGACCGC GGTCTCAAAT GAAGAACAAC ATTAGCGACG TGAAGCTGGG 61 TCTGTTGGCA GCAAAAATCT ACTGGAAAAG CTGGCGCTTC CTGGAACTTA CCGAAGATGA 121 TATCATCTCA ATCGCCCTGC ACGCGGAGCA GGATTCCAAA AAGCGCTTTA ACCCGGAGTT 181 TGGTCTGTCC TTTGATAATT ATCTGAAGCT GAATGGCGCA AATTTCATCC GTAGCAGCTT 241 CCGCTCTATG GTTAATAAAG TCGAGCTGTT GGATAGCAAA AGCAAGTATA GCTTGGAAAA 301 GCAGAATACG GTACTGAATA CGCCGGAAAA CTACCTGAGA TCCCTGGAGT TCAAAGAGAT 361 CATTACCAAA GCGTTCAACA AAGCGAAGAA CGATCAGGAG CGTAAAGTTT TCAGCCTGTA 421 TGTCAAGGGT TATAAAAATT TCGAAATCGC GAAAAAACTG AACATCAGCC CACGTCGCGT 481 CCGTTATCTG CTGGACCTGT TCAAATCCTA CATCAAGCTG CTGACGGAGC GTTACGGTTA 541 CTGAAGAGCT TAGAGACCGC TTGACGGAGC ATCTATCTCG CTGAGGTGTC AATCGTCGGA 601 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT 661 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT 721 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA 781 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 841 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 901 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 961 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 1021 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 1081 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 1141 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 1201 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 1261 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 1321 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT 1381 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 1441 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 1501 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 1561 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 1621 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC 1681 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 1741 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 1801 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 1861 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 1921 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 1981 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 2041 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 2101 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 2161 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 2221 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 2281 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 2341 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 2401 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 2461 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC 2521 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG //