LOCUS       Exported     2881 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2881
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      919..966
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2814..2830
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2831..2862
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..847
                     /label="MG_RS02600"
                     /note=""
                     /translation="MKLRKIFLLPLISLSTLSVACSSTDPGLEKAQAYFQRNNIELSKNNAVTFLKKGYSSDKEEVINTVFASWKTTLLDYQLLEKPLDYSRFAKAFGVNKSKEDVTPNISAKGLYFDETYPGISGQIALVLGVKSQKVVNFQYSWKNNLDFKVQIHLKMTGIVGSDNTSTSLIKSFLASTSGVSESDFTGDKANFDGDVIFTYTPPTDNRRVSESTFSSIPASINFPFDIKIDMSTSHEKLNLLLSTNEQVKKIRTRTFKGKSIDLLPFFYYTLL"
     primer_bind     complement(971..987)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1054..1642)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1833..2693)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2694..2798)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GAAACTGCGT AAAATCTTCC TGCTGCCGCT
       61 GATCAGCCTG TCGACCCTGT CTGTCGCGTG TAGCTCAACG GACCCGGGTC TGGAAAAGGC
      121 TCAAGCATAC TTTCAGCGTA ATAATATTGA ACTGTCTAAG AATAACGCGG TTACCTTCTT
      181 GAAAAAAGGT TACAGCAGCG ACAAGGAGGA GGTTATCAAT ACCGTCTTTG CGAGCTGGAA
      241 AACCACCTTG CTGGATTATC AGCTGTTGGA GAAACCGCTG GATTATAGCC GCTTCGCGAA
      301 AGCCTTCGGC GTGAACAAAA GCAAGGAGGA TGTTACCCCG AACATTAGCG CCAAAGGTCT
      361 GTACTTTGAT GAAACCTACC CGGGCATCAG CGGCCAGATT GCGCTGGTCC TGGGCGTGAA
      421 AAGCCAAAAG GTTGTGAATT TCCAATACAG CTGGAAAAAT AACCTGGATT TCAAAGTACA
      481 GATTCACCTG AAAATGACTG GTATTGTTGG TAGCGATAAC ACCAGCACCT CTCTGATTAA
      541 GAGCTTCCTG GCAAGCACCT CCGGTGTGTC CGAAAGCGAT TTCACCGGTG ATAAGGCGAA
      601 TTTCGATGGC GACGTTATTT TCACCTACAC CCCGCCGACT GATAACCGCC GTGTGAGCGA
      661 AAGCACCTTC AGCAGCATCC CGGCAAGCAT TAACTTTCCG TTCGACATTA AGATTGATAT
      721 GTCCACCTCA CACGAGAAGT TGAATCTGTT GCTGAGCACC AACGAACAGG TTAAGAAGAT
      781 CCGCACCCGT ACCTTCAAGG GCAAGAGCAT CGATCTGCTG CCATTCTTTT ACTACACGCT
      841 GCTGTGAAGA GCTTAGAGAC CGTGTTCTAT GTCCCCATCG TCGCTGAGGT GTCAATCGTC
      901 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
      961 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1021 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1081 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1141 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1201 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1261 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1321 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1381 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1441 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1501 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1561 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     1621 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     1681 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     1741 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     1801 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     1861 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     1921 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     1981 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2041 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     2101 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2161 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2221 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2281 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2341 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2401 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     2461 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     2521 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     2581 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     2641 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     2701 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     2761 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     2821 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     2881 G
//