LOCUS       Exported     3517 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3517
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1555..1602
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3450..3466
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3467..3498
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1483
                     /label="MG_RS02720"
                     /note="Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)."
                     /translation="MEKIRTRYAPSPTGYLHVGGTRTAIFNFLLAKHFNGEFIIRIEDTDTERNIKEGINSQFDNLRWLGVIADESVYNPGNYGPYLQSQKLAVYKKLAFDLIEKNLAYRCFCSKEKLESDRKQAINNHKTPKYLGHCRNLHSKKITNHLEKNDPFTIRLKINNEAEYSWNDLVRGQITIPGSALTDIVILKANGVATYNFAVVIDDYDMEITDVLRGAEHISNTAYQLAIYQALGFKRIPRFGHLSVIVDESGKKLSKRDEKTTQFIEQFKQQGYLPEALLNFLALLGWHPQYNQEFFNLKQLIENFSLSRVVSAPAFFDIKKLQWINANYIKQLTDNAYFNFIDNYLDVKVDYLKDKNREISLLFKNQITHGVQINELIRESFATKIGVENLAKKSHILFKNIKLFLEQLAKSLQGLEEWKAEQIKTTINKVGAVFNLKGKQLFMPIRLIFTNKEHGPDLAHIIEIFDKESAINLIKQFINATNLF"
     primer_bind     complement(1607..1623)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1690..2278)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2469..3329)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3330..3434)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GGAGAAAATT CGTACCCGTT ACGCGCCGAG
       61 CCCGACTGGT TACTTACACG TTGGCGGTAC CCGTACCGCC ATCTTCAACT TCCTGCTGGC
      121 GAAACACTTC AACGGTGAGT TCATCATCCG CATTGAGGAT ACCGACACGG AACGCAACAT
      181 CAAGGAAGGC ATTAACTCTC AATTTGATAA TCTGCGTTGG CTGGGTGTTA TTGCGGACGA
      241 AAGCGTGTAT AACCCAGGCA ATTATGGTCC GTATCTGCAA AGCCAGAAGC TGGCGGTGTA
      301 CAAGAAACTG GCGTTCGATC TGATTGAGAA GAATCTGGCT TACCGTTGTT TCTGCAGCAA
      361 AGAAAAATTG GAATCCGACC GTAAACAGGC AATTAATAAC CACAAAACGC CTAAGTACTT
      421 GGGCCACTGC CGTAACTTGC ACAGCAAGAA AATCACTAAT CACCTGGAGA AGAACGACCC
      481 GTTCACCATT CGTCTGAAAA TTAATAACGA AGCGGAATAT TCCTGGAATG ATCTGGTGCG
      541 TGGCCAAATT ACGATCCCGG GTAGCGCGTT GACCGACATC GTCATTCTGA AGGCAAACGG
      601 TGTTGCAACG TACAACTTCG CAGTGGTTAT TGATGACTAC GACATGGAAA TCACCGATGT
      661 GCTGCGCGGC GCCGAGCATA TCTCCAATAC GGCCTACCAA CTGGCGATCT ATCAAGCGCT
      721 GGGTTTCAAA CGCATCCCGC GTTTTGGCCA CCTGAGCGTT ATTGTTGATG AGTCCGGTAA
      781 AAAACTGTCT AAGCGTGATG AAAAAACCAC CCAGTTCATC GAGCAATTCA AGCAGCAAGG
      841 CTATCTGCCG GAGGCACTGC TGAACTTTCT GGCACTGCTG GGCTGGCATC CGCAGTACAA
      901 CCAAGAGTTC TTTAATCTGA AACAGCTGAT CGAGAACTTC AGCCTTAGCC GCGTGGTGTC
      961 TGCGCCGGCG TTCTTTGATA TTAAAAAACT GCAGTGGATC AACGCTAATT ACATTAAGCA
     1021 GCTGACCGAT AATGCATACT TCAACTTCAT TGATAATTAC CTGGACGTTA AAGTGGACTA
     1081 CTTGAAAGAT AAGAACCGTG AAATCAGCCT GCTGTTCAAA AATCAGATTA CGCATGGTGT
     1141 CCAAATTAAC GAACTGATCC GCGAAAGCTT TGCCACGAAA ATTGGCGTTG AAAATTTGGC
     1201 TAAAAAGAGC CACATCCTGT TCAAGAATAT CAAGCTGTTC TTGGAGCAAT TAGCGAAAAG
     1261 CCTGCAGGGC CTGGAAGAGT GGAAAGCGGA GCAGATTAAG ACCACTATTA ACAAAGTGGG
     1321 CGCAGTGTTC AACCTGAAGG GCAAGCAATT GTTCATGCCG ATCCGTTTGA TCTTTACGAA
     1381 CAAGGAACAC GGTCCGGACC TGGCGCATAT CATTGAAATT TTTGATAAAG AAAGCGCAAT
     1441 TAATCTGATT AAACAGTTCA TCAACGCAAC CAATCTGTTT TGAAGAGCTT AGAGACCGTA
     1501 TGTCCCCTAA GCTCTGGCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1561 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1621 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1681 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1741 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1801 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1861 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1921 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1981 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     2041 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     2101 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     2161 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2221 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2281 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2341 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2401 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2461 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2521 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2581 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2641 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2701 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2761 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2821 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2881 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2941 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     3001 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     3061 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     3121 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     3181 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3241 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     3301 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3361 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3421 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3481 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//