LOCUS Exported 3220 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3220 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1258..1305 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3153..3169 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3170..3201 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1186 /label="MG_RS02730" /note="Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins (By similarity)." /translation="MPIKLAQTNKEIKTTFNPFWSAAVVNEKNNWKNFKKFSAIFIKVIKVFIFIFLTIVGLWGCTQTLAQPWTGTNQVLGSGLEIGYKFGTTGDYRYDLISNNFGPYFTFSDYTLAYGPFYGWFVWPAAQIVLPIMYATRVPLGSGVELGFNMILSLIVLLLLVRLITIVITLNSTLALEKMNEVQGKLAEINAKYKGAIDLQSKRNRQLEIMSLYKKHNIKSSAAFVQVFVTLPIFLIIYRIVTTLRPIKAIILFNFWDLSKVPLTEIFSNFTTTGWPFIIFLVIVLPVQFLSQKLPQVWASKRNENAKAHSQKSIEQLNKTKKMQLIFYFVFAAITAFSAAGVGVYWFLNALFTLLQSYLTHVFIVKRREKRKQNYSKLDLILERE" primer_bind complement(1310..1326) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1393..1981) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2172..3032) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3033..3137) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GCCGATTAAG CTGGCTCAAA CGAATAAGGA 61 GATTAAAACG ACGTTCAACC CGTTTTGGAG CGCAGCAGTC GTTAACGAAA AGAACAATTG 121 GAAAAACTTC AAAAAATTCA GCGCAATTTT TATCAAGGTT ATCAAAGTTT TTATCTTTAT 181 CTTCCTCACC ATCGTCGGCC TGTGGGGTTG CACGCAAACG CTGGCGCAGC CATGGACTGG 241 CACGAACCAA GTCTTGGGTA GCGGCTTGGA GATTGGCTAC AAATTTGGTA CCACGGGCGA 301 CTACCGCTAT GATCTGATCA GCAACAACTT CGGTCCGTAC TTCACGTTCA GCGACTATAC 361 CCTGGCCTAC GGCCCGTTTT ACGGTTGGTT TGTGTGGCCG GCTGCCCAAA TCGTGCTCCC 421 AATCATGTAT GCTACCCGCG TGCCGCTGGG TTCAGGCGTC GAGCTGGGTT TTAACATGAT 481 CCTGAGCCTG ATCGTTCTGC TGTTGCTTGT TCGCCTGATC ACTATTGTCA TCACCCTGAA 541 CAGCACGCTG GCTCTGGAGA AAATGAACGA GGTTCAAGGC AAGCTGGCTG AGATCAACGC 601 GAAGTATAAA GGTGCTATTG ACTTGCAAAG CAAGCGTAAC CGTCAACTGG AGATCATGAG 661 CCTGTACAAA AAGCACAATA TTAAAAGCTC CGCAGCATTC GTCCAGGTGT TCGTTACGCT 721 GCCGATCTTT TTGATTATCT ATCGTATCGT CACCACCCTG CGCCCTATTA AAGCAATTAT 781 TCTGTTCAAT TTTTGGGATT TAAGCAAGGT CCCTCTGACT GAGATTTTCA GCAACTTTAC 841 GACCACCGGT TGGCCGTTCA TTATCTTTTT GGTTATCGTG CTCCCGGTTC AGTTCCTGAG 901 CCAAAAGCTG CCGCAAGTTT GGGCCTCCAA ACGTAATGAG AACGCGAAGG CCCACAGCCA 961 GAAAAGCATT GAACAGCTGA ATAAGACCAA GAAAATGCAA CTGATCTTTT ACTTCGTGTT 1021 CGCGGCTATC ACCGCGTTTA GCGCGGCCGG TGTCGGCGTG TATTGGTTCC TGAATGCGCT 1081 GTTTACCCTG CTGCAGAGCT ACCTGACCCA TGTTTTCATT GTGAAGCGTC GTGAAAAGCG 1141 TAAACAAAAC TATTCCAAGT TGGATCTGAT TCTGGAGCGT GAGTGAAGAG CTTAGAGACC 1201 CCTACGGTTA AGGCTTGTCT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA 1261 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT 1321 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA 1381 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 1441 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 1501 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 1561 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 1621 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 1681 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 1741 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 1801 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 1861 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 1921 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 1981 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 2041 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 2101 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 2161 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 2221 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 2281 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA 2341 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 2401 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 2461 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 2521 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 2581 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 2641 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 2701 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 2761 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 2821 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 2881 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 2941 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 3001 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC 3061 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 3121 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC 3181 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG //