LOCUS       Exported     3220 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3220
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1258..1305
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3153..3169
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3170..3201
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1186
                     /label="MG_RS02730"
                     /note="Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins (By similarity)."
                     /translation="MPIKLAQTNKEIKTTFNPFWSAAVVNEKNNWKNFKKFSAIFIKVIKVFIFIFLTIVGLWGCTQTLAQPWTGTNQVLGSGLEIGYKFGTTGDYRYDLISNNFGPYFTFSDYTLAYGPFYGWFVWPAAQIVLPIMYATRVPLGSGVELGFNMILSLIVLLLLVRLITIVITLNSTLALEKMNEVQGKLAEINAKYKGAIDLQSKRNRQLEIMSLYKKHNIKSSAAFVQVFVTLPIFLIIYRIVTTLRPIKAIILFNFWDLSKVPLTEIFSNFTTTGWPFIIFLVIVLPVQFLSQKLPQVWASKRNENAKAHSQKSIEQLNKTKKMQLIFYFVFAAITAFSAAGVGVYWFLNALFTLLQSYLTHVFIVKRREKRKQNYSKLDLILERE"
     primer_bind     complement(1310..1326)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1393..1981)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2172..3032)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3033..3137)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GCCGATTAAG CTGGCTCAAA CGAATAAGGA
       61 GATTAAAACG ACGTTCAACC CGTTTTGGAG CGCAGCAGTC GTTAACGAAA AGAACAATTG
      121 GAAAAACTTC AAAAAATTCA GCGCAATTTT TATCAAGGTT ATCAAAGTTT TTATCTTTAT
      181 CTTCCTCACC ATCGTCGGCC TGTGGGGTTG CACGCAAACG CTGGCGCAGC CATGGACTGG
      241 CACGAACCAA GTCTTGGGTA GCGGCTTGGA GATTGGCTAC AAATTTGGTA CCACGGGCGA
      301 CTACCGCTAT GATCTGATCA GCAACAACTT CGGTCCGTAC TTCACGTTCA GCGACTATAC
      361 CCTGGCCTAC GGCCCGTTTT ACGGTTGGTT TGTGTGGCCG GCTGCCCAAA TCGTGCTCCC
      421 AATCATGTAT GCTACCCGCG TGCCGCTGGG TTCAGGCGTC GAGCTGGGTT TTAACATGAT
      481 CCTGAGCCTG ATCGTTCTGC TGTTGCTTGT TCGCCTGATC ACTATTGTCA TCACCCTGAA
      541 CAGCACGCTG GCTCTGGAGA AAATGAACGA GGTTCAAGGC AAGCTGGCTG AGATCAACGC
      601 GAAGTATAAA GGTGCTATTG ACTTGCAAAG CAAGCGTAAC CGTCAACTGG AGATCATGAG
      661 CCTGTACAAA AAGCACAATA TTAAAAGCTC CGCAGCATTC GTCCAGGTGT TCGTTACGCT
      721 GCCGATCTTT TTGATTATCT ATCGTATCGT CACCACCCTG CGCCCTATTA AAGCAATTAT
      781 TCTGTTCAAT TTTTGGGATT TAAGCAAGGT CCCTCTGACT GAGATTTTCA GCAACTTTAC
      841 GACCACCGGT TGGCCGTTCA TTATCTTTTT GGTTATCGTG CTCCCGGTTC AGTTCCTGAG
      901 CCAAAAGCTG CCGCAAGTTT GGGCCTCCAA ACGTAATGAG AACGCGAAGG CCCACAGCCA
      961 GAAAAGCATT GAACAGCTGA ATAAGACCAA GAAAATGCAA CTGATCTTTT ACTTCGTGTT
     1021 CGCGGCTATC ACCGCGTTTA GCGCGGCCGG TGTCGGCGTG TATTGGTTCC TGAATGCGCT
     1081 GTTTACCCTG CTGCAGAGCT ACCTGACCCA TGTTTTCATT GTGAAGCGTC GTGAAAAGCG
     1141 TAAACAAAAC TATTCCAAGT TGGATCTGAT TCTGGAGCGT GAGTGAAGAG CTTAGAGACC
     1201 CCTACGGTTA AGGCTTGTCT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1261 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1321 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1381 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1441 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1501 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1561 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1621 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1681 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1741 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1801 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1861 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1921 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1981 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2041 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2101 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2161 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2221 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2281 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2341 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2401 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2461 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2521 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2581 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2641 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2701 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2761 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     2821 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     2881 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     2941 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     3001 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3061 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3121 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3181 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//