LOCUS       Exported     2716 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2716
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      754..801
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2649..2665
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2666..2697
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..682
                     /label="MG_RS02765"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
                     /translation="MSINNKKKRFFFDLAQDDDPEQSLLEEDLFSIKEKVTPQNKNKFNQKQNLSKREETSFDKDSSSFDFGPLSAFEKTFPKSFWSKNNSFQQPEQINQQNENNLENWNEEKSFSSNFSGKPKKERYFQEEQNFQYHNNGFLQQKNQNFQQQFTQPQPIPPQPKTKIPGIVPVERQEKLDTGVHFYTSQRVWQKVRNYSKAVNIPISRILTIILDNVIEE"
     primer_bind     complement(806..822)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(889..1477)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1668..2528)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2529..2633)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AATCAGTTTC TTTGGCAGCC GGTCTCAAAT GAGCATCAAC AATAAGAAAA AACGCTTCTT
       61 TTTCGACCTG GCCCAAGACG ACGATCCGGA GCAATCTCTG CTGGAGGAGG ATCTGTTCTC
      121 CATTAAAGAA AAAGTTACGC CGCAGAATAA GAACAAATTT AACCAGAAAC AGAATCTGTC
      181 CAAACGCGAG GAAACGAGCT TCGACAAGGA TTCTAGCAGC TTTGACTTCG GTCCGCTGAG
      241 CGCGTTCGAA AAGACTTTTC CTAAGAGCTT CTGGTCCAAG AATAACTCAT TTCAACAACC
      301 AGAACAGATC AACCAGCAAA ACGAGAATAA CCTGGAAAAC TGGAATGAAG AAAAGAGCTT
      361 CAGCAGCAAT TTCAGCGGTA AGCCGAAGAA GGAGCGCTAC TTTCAAGAAG AACAAAACTT
      421 CCAGTATCAC AACAACGGCT TCTTGCAGCA GAAAAACCAG AATTTTCAAC AACAATTCAC
      481 GCAGCCGCAA CCGATTCCGC CGCAGCCGAA AACCAAGATT CCTGGCATTG TTCCGGTCGA
      541 ACGTCAGGAG AAATTGGATA CCGGCGTGCA TTTTTACACG TCCCAACGCG TCTGGCAAAA
      601 AGTCCGCAAT TACAGCAAAG CAGTGAATAT TCCGATTTCG CGTATTCTGA CGATTATTCT
      661 GGATAATGTC ATCGAAGAGT GAAGAGCTTA GAGACCCAGC CTCTGTGTTG TCTGATCGCT
      721 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      781 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
      841 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
      901 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
      961 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1021 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1081 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1141 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1201 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1261 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1321 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1381 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1441 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1501 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1561 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1621 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1681 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     1741 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     1801 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     1861 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     1921 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     1981 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2041 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2101 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2161 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2221 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2281 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2341 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2401 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2461 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2521 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2641 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     2701 TCAGGTGGAG TGGGAG
//