LOCUS Exported 2848 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2848 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 886..933 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2781..2797 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2798..2829 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..814 /note="" /translation="MEYFDAHCHLNCEPLLEQHEKSLANFRLIGLKANVVGTNLTNSQIAVNLAKQHPDLLKAGVGIHPNDVQLFDLKAAQATLKKLVSTHRSFISCIGEYGFDYHYTKDYITQQEQFFLMQFQLAEQYQLVHMLHVRDVHERIYEVLKRLKPKQPVVFHCFSEDTNTALKLLTLREVGLKVYFSIPGIVTFKNAKNLQAALSVIPTELLLSETDSPYLAPVPFRGKTNWPECVVHTVQTIADIKQVPLAEIKQAIVHNAKKLFW" /label="yabD" primer_bind complement(938..954) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1021..1609) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1800..2660) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2661..2765) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGTATGTA TAGCGACCGC GGTCTCAAAT GGAATACTTT GATGCGCACT GCCACCTGAA 61 CTGCGAACCG CTGCTGGAAC AGCATGAGAA ATCGCTGGCT AATTTCCGTC TGATTGGCCT 121 GAAGGCGAAC GTTGTCGGTA CTAACTTGAC CAACAGCCAG ATCGCCGTGA ACCTGGCGAA 181 ACAACATCCA GATCTGTTGA AAGCGGGCGT GGGTATTCAC CCGAACGATG TCCAGCTTTT 241 CGATTTGAAA GCTGCGCAGG CGACCCTGAA AAAGTTGGTG TCCACCCATC GTAGCTTCAT 301 CAGCTGTATT GGTGAGTATG GCTTTGATTA CCATTACACC AAAGACTACA TCACCCAACA 361 GGAACAGTTT TTTCTGATGC AATTTCAGTT GGCAGAGCAG TATCAGCTGG TTCACATGTT 421 GCATGTTCGT GATGTCCACG AACGTATCTA TGAAGTTCTG AAGCGCCTGA AGCCGAAACA 481 GCCGGTCGTT TTTCACTGCT TCTCCGAGGA CACGAATACT GCGTTGAAAC TGTTGACGCT 541 GCGCGAGGTC GGTTTGAAGG TTTACTTCTC TATTCCGGGC ATCGTTACGT TTAAAAATGC 601 GAAAAATCTG CAGGCGGCGC TGTCTGTGAT CCCGACGGAA CTGCTGCTGA GCGAAACCGA 661 CAGCCCGTAC CTGGCGCCTG TTCCGTTCCG TGGTAAGACC AATTGGCCGG AATGTGTTGT 721 GCATACTGTG CAAACCATTG CGGACATCAA ACAGGTTCCG CTGGCCGAGA TCAAACAAGC 781 GATTGTGCAT AATGCAAAGA AGCTGTTCTG GTGAAGAGCT TAGAGACCCG CTGATGGTAA 841 TTAGCCCTCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT 901 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT 961 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA 1021 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 1081 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 1141 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 1201 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 1261 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 1321 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 1381 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 1441 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 1501 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 1561 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT 1621 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 1681 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 1741 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 1801 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 1861 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 1921 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 1981 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 2041 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 2101 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 2161 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 2221 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 2281 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 2341 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 2401 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 2461 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 2521 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 2581 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 2641 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 2701 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 2761 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG 2821 CTTTTGACTT GGTCAGGTGG AGTGGGAG //