LOCUS Exported 2857 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2857 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 895..942 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2790..2806 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2807..2838 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..823 /note="Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane (By similarity)." /translation="MFNLSDFSELPRWIGAEFLGTFFLILSGNGAGSQLTLNKMFAKESKAKLLTAAFAWGIAVLVGVLIANSLFEGAGNINPAVSLFYAVSGTIQKALYPLHVNFSIPLLWVALLLAWVAQFAGAMLAQALLNFLFWKHIEQTDPQSVLVTHCTNPAIFNIPRNFATEFVATSVLIASLLVAGSFGANRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVYWLSPIKNKDPNLKYSWIPVAAPLSASVILGVLVAVIV" /label="glpF" primer_bind complement(947..963) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1030..1618) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1809..2669) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2670..2774) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GTTTAATCTG AGCGATTTTA GCGAACTGCC 61 ACGCTGGATC GGTGCAGAGT TCCTGGGTAC CTTCTTTTTG ATTCTGTCTG GTAATGGCGC 121 GGGCTCACAA CTGACCCTGA ATAAAATGTT CGCCAAGGAA TCGAAAGCCA AACTGCTGAC 181 GGCTGCGTTC GCGTGGGGCA TTGCGGTGCT GGTGGGTGTG CTGATCGCTA ACAGCTTGTT 241 CGAGGGCGCG GGTAACATCA ATCCGGCAGT TAGCCTGTTT TATGCAGTTA GCGGCACCAT 301 TCAGAAAGCG CTCTACCCGC TGCACGTCAA CTTCAGCATC CCGCTGTTAT GGGTGGCCCT 361 GCTGCTGGCT TGGGTGGCGC AATTTGCGGG TGCCATGCTG GCGCAGGCAC TGTTGAATTT 421 TCTGTTTTGG AAACACATCG AGCAAACCGA CCCGCAAAGC GTTCTGGTCA CCCATTGTAC 481 GAACCCGGCA ATCTTTAACA TCCCGCGCAA CTTTGCGACG GAGTTCGTTG CCACCAGCGT 541 TTTGATCGCG TCCCTCCTCG TGGCGGGTAG CTTTGGTGCG AATCGCTTCG ATCAGAGCCC 601 GCGTGGTGTG GTTCCTATGC TGGTTGTTAC CGGTCTGATT ATGAGCTTTG GTGCTGCCAC 661 TGGCACCGCT ATCAACCCTG CACGCGATCT GGGCCCGCGT ATCGTTTATT GGCTGAGCCC 721 GATTAAAAAC AAGGACCCAA ATCTGAAATA CTCGTGGATT CCGGTTGCAG CTCCGCTGAG 781 CGCAAGCGTC ATTCTGGGCG TTCTCGTTGC GGTTATCGTG TGAAGAGCTT AGAGACCGTG 841 TCACATTGTG TCGGACTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA 901 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC 961 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA 1021 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 1081 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 1141 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 1201 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 1261 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 1321 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 1381 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 1441 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 1501 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 1561 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 1621 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 1681 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 1741 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 1801 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 1861 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 1921 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA 1981 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 2041 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 2101 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 2161 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 2221 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 2281 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 2341 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 2401 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 2461 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 2521 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 2581 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 2641 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 2701 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 2761 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC 2821 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG //