LOCUS       Exported     3418 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3418
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1456..1503
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3351..3367
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3368..3399
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1384
                     /label="MPN047"
                     /note=""
                     /translation="MVQTPSEINTHLKHLLACDAYKLSHRLMYPNDTTNLYSCLTARGGRGGFPNFVWNHEFAKKIILEVFGNFCDSVLAVQNDPGLAQALTDKVTTVFGDPQFGLEFTQHICYLANFLKQHHQLPLTVKIHQSSEGLAFRTPLVTITGSDQMVPELVWLVNYFETVLLENIWLYQTTLTVAQSLKLLLERYANETADNTEFTHFQCHDFSMRGMSSLQSALYVANAHLQYFSGSDTILGGVAAKSILASEHSVMCADGQEGELNTFKRLLEQFPNKNLSLVIDSYDMWHVLDNILPQLKDLVLQRQEKLYLRPDSGNFETLICQGKRFNPEDKTTWGVIDYLDYHFGSTVNQKGYKVLNQKLGIVYGDGITYERIEYILEQLKQRGFCSSNIVFGVGSTTYQNLNRDTLGFVYKLTAIKKGNTWHDVTKSPITDPTKQSIGGRFDNPNLIQVYG"
     primer_bind     complement(1508..1524)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1591..2179)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2370..3230)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3231..3335)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GGTGCAGACC CCTTCCGAAA TTAATACGCA
       61 TCTGAAACAC CTCTTGGCGT GCGACGCATA CAAACTGAGC CACCGTCTGA TGTACCCGAA
      121 TGACACCACC AATCTGTATA GCTGCCTGAC CGCACGCGGT GGCCGTGGTG GTTTCCCTAA
      181 CTTTGTGTGG AACCACGAGT TCGCGAAGAA AATTATCCTG GAAGTTTTCG GTAATTTCTG
      241 CGATAGCGTC CTGGCAGTGC AGAATGATCC GGGCTTGGCA CAGGCGCTGA CGGATAAGGT
      301 GACCACGGTT TTTGGCGATC CTCAATTCGG TCTGGAGTTT ACCCAACACA TTTGTTACCT
      361 GGCTAACTTT CTGAAACAGC ACCATCAGTT GCCGCTGACC GTTAAAATCC ACCAGTCCAG
      421 CGAGGGCCTG GCATTCCGTA CCCCATTGGT TACGATTACG GGTAGCGACC AAATGGTTCC
      481 GGAACTGGTC TGGCTGGTCA ACTACTTCGA AACCGTCCTG CTGGAGAATA TCTGGCTGTA
      541 CCAGACCACC CTGACCGTGG CGCAGTCCTT GAAGTTACTG TTGGAACGTT ATGCCAACGA
      601 AACGGCGGAC AACACGGAAT TTACGCACTT CCAATGTCAT GACTTTAGCA TGCGTGGTAT
      661 GAGCAGCTTG CAGAGCGCGT TGTACGTTGC CAACGCCCAT CTGCAGTATT TCTCCGGCAG
      721 CGATACCATC CTGGGTGGCG TTGCGGCGAA ATCCATTCTG GCGTCCGAAC ACAGCGTTAT
      781 GTGTGCCGAT GGTCAAGAAG GTGAATTGAA TACCTTCAAA AGACTGCTGG AGCAGTTCCC
      841 GAATAAGAAC CTGAGCCTGG TCATTGACTC GTACGATATG TGGCACGTGC TGGATAACAT
      901 TCTGCCTCAG CTGAAGGACC TGGTCCTGCA ACGTCAGGAG AAACTTTATC TGCGCCCGGA
      961 TTCCGGTAAT TTCGAGACTC TGATTTGCCA GGGTAAACGT TTCAACCCGG AGGACAAAAC
     1021 CACCTGGGGC GTGATCGACT ATCTGGACTA CCATTTCGGT TCCACTGTGA ACCAAAAGGG
     1081 TTACAAGGTG CTGAACCAAA AGCTTGGTAT CGTTTATGGT GATGGTATCA CCTATGAGCG
     1141 CATCGAATAC ATCCTGGAAC AGTTGAAGCA ACGTGGCTTT TGCAGCAGCA ACATCGTATT
     1201 CGGTGTGGGC TCTACGACCT ACCAGAATCT GAATCGCGAC ACGCTCGGTT TCGTTTACAA
     1261 GTTGACGGCA ATCAAAAAAG GCAATACCTG GCATGACGTC ACTAAGAGCC CGATCACGGA
     1321 CCCGACCAAG CAGAGCATCG GTGGTCGTTT CGACAATCCT AACCTGATTC AGGTGTACGG
     1381 CTGAAGAGCT TAGAGACCGC CCGGCAATTC TTATAGCTCG CTGAGGTGTC AATCGTCGGA
     1441 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1501 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1561 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1621 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1681 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1741 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1801 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1861 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1921 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1981 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     2041 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     2101 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2161 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     2221 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2281 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2341 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2401 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2461 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2521 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2581 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2641 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2701 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2761 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2821 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2881 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2941 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     3001 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     3061 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     3121 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     3181 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3241 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3301 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3361 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//