LOCUS       Exported     3589 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3589
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1627..1674
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3522..3538
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3539..3570
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1555
                     /label="glpK"
                     /note="Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate."
                     /translation="MDLKQQYILALDEGTSSCRTIVFDKDLNQVAIAQNEFNQFFPKSGWVEQDPLEIWSVQLATMQSAKNKAQIKSNNIAAVGITNQRETIVLWNKENGLPVYNAIVWQDQRTASLCDKLNQDTKIKEFVKKHTGLPINPYFSATKIAWILENVPLAQKMLKEDKLLAGTIDTWLIWKLTGGKMHVTDVSNASRTLLFDITTMTWSQELGDIFKVPLSILPKVMPSNAHFGDIVPSHWSTSATGMVPIRGVAGDQQAALFGQLCVEPAMVKNTYGTGCFMLMNIGNELKYSQHNLLTTVAWQLENQKPVYALEGSVFVAGAALKWLRDSLKVMYSAAESDFYAKLAQKEEQEVVFVPAFTGLGAPYWDASARGAIFGIEANTKREHLVKATLEAIAFQANDLIKAMASDLNSSIKKIKADGGACNSNYLMQFQADIANLEVIIPKNVETTTMGAAFLAGLAVNYWKDTKQLEKLTGIAKQFKSQMNQTVREKKSKRWNEAVKRTLKWASLD"
     primer_bind     complement(1679..1695)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1762..2350)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2541..3401)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3402..3506)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GGACCTGAAG CAACAATACA TTTTGGCACT
       61 GGATGAGGGT ACCAGCAGCT GTCGTACGAT CGTGTTCGAT AAGGACCTGA ATCAGGTTGC
      121 GATCGCGCAG AACGAGTTTA ATCAATTTTT TCCGAAATCG GGCTGGGTTG AGCAAGACCC
      181 GCTGGAAATT TGGAGCGTTC AACTGGCCAC CATGCAGTCC GCGAAGAACA AAGCACAAAT
      241 CAAGAGCAAT AATATCGCGG CAGTCGGTAT TACTAACCAA CGTGAAACGA TTGTTCTGTG
      301 GAACAAGGAG AATGGCCTGC CAGTTTACAA TGCGATCGTT TGGCAGGATC AACGTACGGC
      361 ATCCCTGTGT GACAAACTGA ACCAGGACAC CAAGATCAAA GAATTTGTCA AAAAGCACAC
      421 CGGCCTGCCG ATCAATCCGT ATTTCAGCGC GACCAAAATC GCGTGGATCC TGGAAAATGT
      481 TCCGCTGGCG CAAAAAATGT TGAAAGAGGA TAAACTGTTG GCGGGTACGA TCGACACCTG
      541 GCTGATTTGG AAACTGACGG GTGGCAAGAT GCACGTTACC GATGTTAGCA ATGCATCTCG
      601 CACCCTGCTG TTCGATATCA CCACCATGAC GTGGAGCCAG GAACTGGGCG ATATCTTCAA
      661 GGTCCCGCTG AGCATCCTGC CGAAGGTGAT GCCGAGCAAT GCACACTTCG GTGACATCGT
      721 TCCGAGCCAT TGGAGCACCA GCGCGACCGG TATGGTCCCG ATCCGTGGTG TCGCTGGTGA
      781 CCAGCAAGCA GCACTGTTCG GCCAACTGTG CGTGGAACCG GCAATGGTCA AGAACACTTA
      841 CGGCACCGGC TGCTTTATGC TTATGAACAT TGGCAACGAA TTGAAGTACA GCCAGCACAA
      901 CTTGCTGACG ACGGTGGCCT GGCAGCTGGA GAATCAGAAG CCGGTGTACG CGTTGGAGGG
      961 TTCGGTTTTT GTTGCGGGTG CGGCGCTGAA GTGGCTGCGT GATAGCCTGA AAGTTATGTA
     1021 CAGCGCGGCG GAGAGCGACT TTTACGCCAA GCTGGCGCAG AAGGAGGAGC AGGAGGTCGT
     1081 CTTTGTGCCG GCGTTCACGG GTCTGGGTGC ACCATATTGG GACGCCAGCG CGCGTGGTGC
     1141 CATCTTCGGC ATCGAAGCGA ATACTAAGCG CGAGCACTTG GTTAAAGCGA CCCTGGAGGC
     1201 AATCGCGTTC CAGGCGAACG ACCTGATCAA GGCAATGGCG AGCGATCTGA ACAGCAGCAT
     1261 CAAGAAAATC AAGGCGGATG GTGGTGCCTG TAACAGCAAT TACTTGATGC AGTTCCAGGC
     1321 TGATATTGCG AACTTGGAGG TGATTATCCC AAAGAACGTT GAAACTACGA CCATGGGCGC
     1381 GGCGTTCTTG GCCGGTCTGG CGGTCAATTA CTGGAAAGAC ACCAAGCAGC TGGAAAAGCT
     1441 GACCGGCATT GCTAAGCAAT TCAAGAGCCA AATGAATCAG ACGGTTCGTG AGAAAAAGAG
     1501 CAAGCGCTGG AATGAGGCGG TTAAACGTAC CCTGAAGTGG GCGAGCTTGG ATTGAAGAGC
     1561 TTAGAGACCC CATCTTGCGT CGTGAGAATC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1621 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1681 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1741 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1801 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1861 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1921 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1981 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     2041 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     2101 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     2161 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     2221 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     2281 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     2341 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     2401 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     2461 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     2521 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2581 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2641 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2701 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2761 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2821 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2881 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2941 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     3001 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     3061 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     3121 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     3181 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     3241 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     3301 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     3361 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     3421 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3481 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3541 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//