LOCUS Exported 3022 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3022 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 1060..1107 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2955..2971 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2972..3003 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..988 /label="gcp" /note="Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction." /translation="MEQPLCILGIETTCDDTSIGVITESKVQAHIVLSSAKLHAQTGGVVPEVAARSHEQNLLKALQQSGVVLEQITHIAYAANPGLPGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSALIDQDINQLKLPALGLVVSGGHTAIYLIKSLFDLELIAETSDDAIGEVYDKVGRAMGFPYPAGPQLDSLFQPELVKSHYFFRPSTKWTKFSYSGLKSQCFTKIKQLRERKGFNPQTHDWNEFASNFQATIIDHYINHVKDAIQQHQPQMLLLGGGVSANKYLREQVTQLQLPYLIAPLKYTSDNGAMIGFYANLLINGKNN" primer_bind complement(1112..1128) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1195..1783) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1974..2834) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2835..2939) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AACGATGATG CTCACTCTCG GGTCTCAAAT GGAGCAACCG TTGTGCATTC TGGGTATTGA 61 AACGACGTGC GACGATACGT CTATCGGCGT CATCACGGAG AGCAAGGTTC AAGCCCACAT 121 TGTGCTGTCC AGCGCAAAAC TGCATGCACA AACGGGCGGC GTAGTCCCGG AAGTCGCGGC 181 CCGTAGCCAC GAACAGAACT TGTTGAAGGC CCTGCAGCAG AGCGGCGTGG TACTGGAGCA 241 GATCACTCAT ATCGCGTATG CAGCTAACCC GGGCCTGCCG GGTTGCCTGC ACGTTGGTGC 301 GACCTTTGCG CGTTCCCTGA GCTTCCTGCT GGACAAGCCG CTGCTGCCGA TTAATCACCT 361 GTACGCCCAT ATCTTTAGCG CGCTGATTGA CCAAGACATC AATCAACTGA AACTGCCGGC 421 GCTGGGCCTG GTGGTCAGCG GCGGTCACAC TGCTATTTAC CTGATCAAAA GCCTGTTCGA 481 CCTGGAGCTG ATCGCTGAAA CCTCTGATGA TGCAATTGGT GAGGTCTACG ATAAGGTCGG 541 TCGTGCTATG GGTTTCCCGT ACCCGGCGGG TCCGCAGCTG GACAGCCTGT TCCAACCGGA 601 GCTGGTTAAA AGCCATTATT TCTTCCGCCC GAGCACCAAA TGGACCAAAT TTAGCTATAG 661 CGGTTTGAAG TCGCAATGTT TCACCAAGAT CAAGCAATTG CGTGAACGCA AAGGCTTTAA 721 CCCGCAGACC CACGATTGGA ACGAGTTCGC ATCCAACTTC CAGGCAACGA TTATTGATCA 781 CTATATTAAC CATGTGAAAG ACGCAATCCA GCAGCACCAG CCGCAGATGC TGTTGCTGGG 841 TGGTGGCGTG TCCGCCAACA AATATCTGCG CGAACAGGTC ACTCAGCTGC AGCTGCCATA 901 TCTGATTGCG CCGCTGAAGT ATACTAGCGA TAATGGTGCT ATGATTGGCT TTTATGCGAA 961 TCTGCTGATT AATGGCAAGA ATAACTGAAG AGCTTAGAGA CCCCGCGTAA GAGGTTCCTA 1021 TTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 1081 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG 1141 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA 1201 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 1261 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 1321 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 1381 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 1441 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 1501 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 1561 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 1621 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 1681 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 1741 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC 1801 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 1861 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 1921 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 1981 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 2041 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 2101 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 2161 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 2221 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 2281 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 2341 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 2401 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 2461 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 2521 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 2581 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 2641 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 2701 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 2761 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 2821 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 2881 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 2941 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG 3001 ACTTGGTCAG GTGGAGTGGG AG //