LOCUS       Exported     3664 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3664
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1702..1749
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3597..3613
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3614..3645
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1630
                     /translation="MVKVWKIGFGVFLPTALLFSACSFKDYIPTPSFRKDFSTENNFVKNKVPGKDDIYSKFYDLTFSLNFVNNQAQEFGTGWLIDWKGDENKNLSKNKEGQTASQTRSSSEQTTDQDANLFTAYIATNLHVADGLKNDQDYAPYNKDGWGQPYPYQQKTQSFLLGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFWTAGKYNVYNGGNSIGNYADFAVIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGTVNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLSRFILQYHNVNYKQFGYGTILWDTNFGGGSSGSAIFNQNKQINSIYFGALVNVTTDRNENVGLGLGQILRAPNTFNSSHEVPYSYDLIFGDVNTTNFYAQFAKKHNTHMWSKIQSTQNGEIGFHKNSKTGQQRH"
                     /label="MPN083"
                     /note=""
     primer_bind     complement(1754..1770)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1837..2425)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2616..3476)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3477..3581)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GGTCAAAGTT TGGAAAATCG GTTTCGGCGT
       61 GTTTCTGCCG ACGGCTCTGC TGTTTTCAGC GTGCAGCTTT AAGGATTACA TCCCGACCCC
      121 GAGCTTTCGT AAGGATTTCA GCACCGAGAA TAACTTCGTT AAAAACAAAG TTCCGGGTAA
      181 AGACGATATC TATAGCAAAT TCTACGACCT GACGTTTTCT CTGAACTTTG TTAATAATCA
      241 GGCTCAAGAA TTCGGCACTG GTTGGCTGAT CGATTGGAAA GGTGACGAGA ATAAGAATCT
      301 GTCTAAAAAC AAGGAAGGCC AAACCGCGAG CCAAACGCGT AGCAGCAGCG AACAAACCAC
      361 GGACCAAGAT GCAAACCTGT TCACGGCCTA TATTGCGACG AATCTGCACG TTGCGGACGG
      421 TTTGAAGAAC GACCAGGACT ATGCCCCGTA CAATAAGGAT GGCTGGGGTC AGCCGTATCC
      481 GTACCAGCAA AAAACCCAGA GCTTCCTGCT GGGCAAATAC ACCAAACCGA ACGTTCAGCT
      541 GGTTAAAACG AATTATGAGA AACCAGAGGA CGCAGTTATC GAACAGAAAC TGAAGGAGGA
      601 TAGCCTGCTG TTTATTCAGA CCAGCACGCT GCCTAAGACC GCATACGCGG CAATCGACCC
      661 TGTTAACTTC AGCTATAACC CGACCCGTAC CAATGGTTTC TGGACCGCCG GTAAGTATAA
      721 CGTGTACAAT GGTGGCAACA GCATTGGCAA CTACGCTGAT TTCGCGGTTA TCGAAGTCCC
      781 TCTGGTCTTG AGCAATCCTA ACGACGCGAA GATTTACCAA GAATGGATCC GTCCGGCAAC
      841 CCAAGCCTAT AAGTATCTGG GCGACGTGGA GGGTCTGTTC GCGAAGAAAG GTTACCGTAG
      901 CTATATCCAA GACTTTTACC ATCTGCTGGG TTATCCAGTC ACGAAGAACA CGAAATCCGA
      961 GTTCATCCTT GGCCAGAGCC AGGGCACCGT TAATCACATG TCTTTCTCGA ACGATGAATC
     1021 AAATACCACG AATACCATTA CCAAGGCCAG CCTGACCCAA CAGCCACATA AAGAGCAGAG
     1081 CGCGTACGTG GTCCGTGAGA GCGGCCTGCC GACCCTGACG ATGAATGTCG ATAAATACAC
     1141 GGGCGCGAAG GGCACCCACC TGGTCAATGT TGATCAAATT ACGGACCTGA GCCTGGGTGA
     1201 TGGTTTGATT GATTTTGGTG GTCTGAGCCG TTTCATCCTG CAGTATCATA ACGTCAACTA
     1261 CAAACAATTT GGTTATGGCA CGATCCTGTG GGATACGAAC TTCGGTGGCG GTTCTTCCGG
     1321 TTCCGCGATC TTCAATCAGA ACAAGCAGAT CAATTCGATC TATTTCGGCG CTCTGGTTAA
     1381 CGTGACGACC GACCGCAACG AAAACGTCGG CCTGGGCCTC GGTCAGATTC TGCGTGCGCC
     1441 GAACACCTTC AATTCCTCCC ATGAAGTCCC GTATTCTTAC GATCTGATCT TCGGTGATGT
     1501 GAACACCACT AATTTCTACG CACAATTCGC AAAGAAGCAT AATACCCATA TGTGGTCGAA
     1561 GATCCAATCT ACCCAAAATG GCGAAATCGG TTTCCACAAA AACAGCAAAA CTGGTCAACA
     1621 ACGTCATTGA AGAGCTTAGA GACCCTGTAT CATGGTTCCC TCGGCGCTGA GGTGTCAATC
     1681 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1741 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1801 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1861 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1921 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1981 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     2041 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     2101 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     2161 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2221 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2281 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2341 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2401 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     2461 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2521 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2581 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2641 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2701 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2761 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2821 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2881 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2941 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     3001 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     3061 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     3121 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     3181 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     3241 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     3301 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     3361 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     3421 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3481 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3541 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3601 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG
     3661 GGAG
//