LOCUS       Exported     3385 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3385
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1423..1470
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3318..3334
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3335..3366
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1351
                     /label="MPN085"
                     /note=""
                     /translation="MQLTTSQQMQTNFLTTNKHHLLKYTNGLIWCWWLFVISLVLASSTFRGFFLGTINIVNFVFWILALIFGVAVAFINGVLSSELKENSVFQEEQKRFFLGFFFPQMAFCNALWLKLKLSYLNSERENLLEKIKQKLKKLTLSVFVVWGIYCVLATSIYLPNALRILNIYQIPNLIALINNRLSEILPDGNRYFLGHSSFAFHYYEIVSRIPFLVFFIIPTITLITLGCYLFAYLRFINSNKLRKPLSTLSIVIMLTDVVGIIQWIIIDILLIWLNVPFVIFVIFWVIKLVLPLAMIGTFVSSLTIYKKVTSKEWLAIKEEQINLTTMNININMGEQSSKNMNSFENHESNERNSLQIYQQHSSMMSETKRKQSSLSYDARILLPKSPYNTKKTLFLIIFFSIISLILATIGSVFISFAIVQISIPFYVIGGVIWFFTFISL"
     primer_bind     complement(1475..1491)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1558..2146)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2337..3197)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3198..3302)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GCAACTGACT ACCTCCCAGC AGATGCAGAC
       61 GAACTTTCTG ACCACGAACA AACACCATCT GCTGAAGTAT ACCAATGGTC TGATCTGGTG
      121 CTGGTGGCTG TTTGTGATCT CCCTCGTTCT GGCAAGCTCG ACCTTTCGCG GTTTCTTTCT
      181 GGGCACGATT AACATCGTGA ACTTCGTGTT TTGGATCCTG GCGCTGATCT TCGGTGTTGC
      241 CGTCGCGTTT ATCAATGGTG TCCTGAGCTC AGAACTGAAA GAGAACAGCG TTTTCCAAGA
      301 GGAGCAAAAA CGTTTCTTCC TGGGTTTCTT TTTCCCACAA ATGGCTTTCT GCAACGCGCT
      361 GTGGTTGAAA CTGAAACTGT CCTATCTGAA TAGCGAGCGT GAGAATCTGC TGGAAAAAAT
      421 CAAGCAGAAA CTCAAGAAAC TGACCCTGTC CGTATTCGTA GTGTGGGGTA TCTACTGTGT
      481 TCTGGCCACG AGCATTTATC TGCCGAATGC GTTGCGCATC CTGAACATTT ACCAGATCCC
      541 GAACCTGATC GCGCTGATCA ACAACCGCCT GAGCGAGATT CTGCCGGATG GTAATCGTTA
      601 CTTTCTGGGT CACTCCTCCT TCGCCTTTCA CTATTACGAG ATTGTTTCGC GCATCCCGTT
      661 TTTGGTCTTT TTTATCATCC CGACGATTAC CTTGATTACC CTGGGCTGCT ATCTGTTTGC
      721 GTACCTTCGC TTTATTAATT CCAACAAGCT GCGTAAGCCG CTGAGCACCT TAAGCATTGT
      781 TATCATGCTG ACGGATGTCG TGGGCATTAT CCAGTGGATC ATCATCGACA TTCTGTTGAT
      841 CTGGCTGAAT GTCCCGTTTG TTATTTTCGT CATCTTTTGG GTGATCAAGC TGGTACTGCC
      901 TCTGGCCATG ATTGGTACCT TTGTTAGCTC TCTTACTATT TATAAGAAGG TTACCTCTAA
      961 GGAATGGCTG GCTATTAAGG AGGAGCAAAT CAATCTGACC ACGATGAATA TCAATATCAA
     1021 TATGGGCGAG CAAAGCAGCA AGAACATGAA CAGCTTCGAG AACCATGAGA GCAACGAACG
     1081 TAACAGCCTG CAGATCTACC AACAGCACAG CAGCATGATG AGCGAAACCA AACGCAAACA
     1141 GTCTAGCCTG TCATACGATG CGCGTATTCT GCTGCCAAAG AGCCCATACA ATACGAAAAA
     1201 GACGTTGTTC CTGATTATCT TTTTCTCCAT CATTTCTCTG ATTCTGGCGA CCATTGGTTC
     1261 CGTGTTCATC TCTTTTGCAA TTGTCCAAAT TAGCATCCCG TTTTATGTGA TCGGCGGCGT
     1321 CATTTGGTTC TTCACGTTTA TCAGCCTGTG AAGAGCTTAG AGACCGTGTC GCTCGCAATC
     1381 CTAATCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1441 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1501 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1561 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1621 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1681 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1741 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1801 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1861 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1921 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1981 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     2041 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     2101 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     2161 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     2221 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     2281 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     2341 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     2401 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     2461 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     2521 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     2581 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     2641 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2701 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     2761 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     2821 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     2881 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     2941 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     3001 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     3061 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     3121 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     3181 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     3241 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     3301 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     3361 TTGACTTGGT CAGGTGGAGT GGGAG
//