LOCUS Exported 3070 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3070 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1108..1155 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3003..3019 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3020..3051 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1036 /note="The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity (By similarity)." /translation="MGRIKTYDFDGEYVTWTTRWSYAGSIYYRNGKFSASSNCGILKVLNKEINPKFLAYALKKEAKKFVNTTSAIPILRTQKVVEIPIDFPPLQIQEKIATILDTFTELSAELSAELSAELSAELSAELRERKKQYAFYRDYLLNLKNWKEENKYYKLGEIAQKVLVGGEKPADFSKEKNEVYKYPILSNNSKAEEFLVYSKTFRVEEKSITVSARGTIGAVFYRDFAYLPAVSLICFVPKEEFDIRFLFHALRAIKFKKQGSATGQLTVAQFKEYGIHVPSLKKQKEIAAILDPLYSFFTDLNEGIPAEIELRKKQLDYYQNFLFNWVQNQKAASIL" /label="hsdS" primer_bind complement(1160..1176) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1243..1831) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(2022..2882) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2883..2987) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GGGCCGCATC AAAACCTACG ACTTCGATGG 61 TGAGTACGTG ACGTGGACCA CCCGCTGGTC TTACGCTGGC AGCATCTACT ATCGCAATGG 121 CAAATTTAGC GCCAGCAGCA ATTGCGGCAT TCTGAAGGTG TTAAATAAAG AAATTAATCC 181 GAAGTTCCTG GCGTATGCCC TCAAAAAAGA GGCGAAAAAG TTCGTTAATA CCACCAGCGC 241 GATTCCGATC TTGCGCACCC AGAAAGTTGT GGAAATCCCA ATTGATTTTC CGCCGCTGCA 301 AATCCAAGAA AAGATCGCGA CCATCTTGGA CACCTTTACC GAGCTGAGCG CGGAACTGAG 361 CGCAGAGCTG TCCGCTGAGC TGAGCGCGGA GCTGAGCGCC GAGCTGCGTG AGCGTAAGAA 421 GCAGTATGCA TTTTACCGTG ATTATCTGCT GAACCTGAAG AACTGGAAAG AAGAAAACAA 481 GTATTATAAG CTGGGTGAGA TCGCCCAGAA AGTGCTGGTT GGTGGCGAAA AGCCGGCGGA 541 CTTCAGCAAG GAGAAGAACG AAGTTTACAA ATACCCGATT CTGAGCAACA ACTCTAAGGC 601 GGAAGAGTTC CTGGTCTATT CCAAAACCTT TCGTGTCGAA GAAAAAAGCA TCACGGTGTC 661 CGCACGTGGC ACCATTGGCG CGGTATTTTA CCGTGACTTC GCCTATCTTC CGGCTGTTAG 721 CCTGATTTGC TTCGTGCCGA AAGAGGAGTT CGATATCCGT TTTCTGTTCC ATGCGCTCCG 781 TGCAATTAAA TTCAAGAAGC AGGGTTCGGC CACCGGTCAG CTGACCGTGG CGCAGTTCAA 841 GGAATATGGT ATCCACGTCC CGTCTTTGAA GAAACAAAAG GAGATTGCAG CGATTCTGGA 901 CCCTCTGTAT AGCTTCTTCA CCGACCTGAA TGAAGGCATC CCGGCCGAGA TTGAGCTGCG 961 TAAAAAGCAG CTGGATTACT ATCAGAATTT CCTGTTCAAT TGGGTCCAAA ATCAGAAAGC 1021 GGCGTCCATT CTGTGAAGAG CTTAGAGACC GTCTCATGCT AGCCACGTAT CGCTGAGGTG 1081 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1141 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 1201 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 1261 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 1321 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 1381 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 1441 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 1501 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 1561 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 1621 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 1681 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 1741 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 1801 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT 1861 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 1921 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 1981 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 2041 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 2101 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 2161 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 2221 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 2281 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 2341 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 2401 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 2461 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 2521 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 2581 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 2641 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 2701 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 2761 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 2821 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 2881 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 2941 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT 3001 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT 3061 GGAGTGGGAG //